Protein purification

Inmaculada Garcia garciai at
Thu Mar 21 05:02:15 EST 1996

 I have just tried to purify one protein from a SDS-PAGE gel, I cut one 
band of around 25 KDa, then I followed one protocol from AMICON, that 
includes a filtering step to remove the gel, and then after concentration 
of the sample I loaded it again to see the results. I have seen that the 
protein has become now bigger, around 60 KDa or more, does anyone have 
any idea if this is a kind of aggregation? and how it happens?

 Thanks in advance (could you mail me to garciai at

    Inmaculada Garcia Robles

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