RNA isolation/Chomozynski

Ferland Louis H. ferlandl at ERE.UMontreal.CA
Thu Mar 21 04:20:04 EST 1996


On 15 Mar 1996, Susan Patrick wrote:

> Date: 15 Mar 1996 21:09:24 GMT
> From: Susan Patrick <patrics at okstate.edu>
> To: methods at net.bio.net
> Subject: RNA isolation/Chomozynski
> 
> We are using a modification of the Chomczynski single-step method of RNA 
> isolation.  We have n-lauroylsarcosine and the sodium salt.  Our 
> solution calls for 0.5% sarcosyl.  Is this needed in percent weight or 
> by volume?
> 
> 
grams per 100 ml.


Dr. Louis H. Ferland
Centre de Recherche, Hotel-Dieu de Montreal
Dept de Nutrition, Universite de Montreal
Phone: (514) 843-2757     FAX: (514) 843-2719




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