chickens vs rabbits for antibodies
Richard Van Frank
vanfrank at iquest.net
Thu Mar 21 09:54:41 EST 1996
In article <lfliegel-200396124514 at fliegel-2.cvascular.med.ualberta.ca>,
lfliegel at gpu.srv.ualberta.ca (L. Fliegel) wrote:
>In article <sbv-190396124419 at path22304.path.cwru.edu>, sbv at pop.cwru.edu
>(Scott Vande Pol) wrote:
>> Some friends of mine have been making antibodies in chickens instead of
>> rabbits. But I have yet to get a clear consensus as to if it is a s
>> reliable as rabbits. Cost is clearly better though. What is the
>> experience of those of you out there who have done both? If you were
>> successful did you use peptides or bacterial expressed proteins as your
>> immunogen? Any experience you could share would be appreciated.
>> Scott Vande Pol
>> Case Western Reserve University
>> Department of Pathology
>> Cleveland Oh. 44106
>> sbv at pop.cwru.edu
>Chickens were a mess, a real nightmare for me and several others I have
>spoken to. They made lousy (no) antibodies, were a pain to inject. They
>were supposed to be easy and deposit antibodies in their eggs which are
>then purified. As soon as the chickens were injected they stopped laying
>eggs. Bleeding chickens is a mess and they didn't produce any antibodies.
> I have always had better
>success with rabbits.
Years ago I used chickens to produce antibodies to viral antigens with sucess.
I did not try to recover antibodies from the eggs but bled the chickens from
the wing vein, this very easy to do, or from the hart. It is best to you young
chickens, easier to handle, require less room etc. etc.
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