Fog in In Vitro Translations

John JPFELL00 at ukcc.uky.edu
Fri Mar 22 09:57:56 EST 1996


 
We are trying to translate protein from pBluescript derived RNA.  We have used
both rabbit retic and wheat germ lysates, but get a fog of 35S label that goes
from about 40 kD to the bottom of the gel.  Does anyone know how to prevent
this or get rid of it?  thanks.
 
John Fellers
University  of Kentucky



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