GST Fusion stuck on sepharose

John JPFELL00 at
Fri Mar 22 09:53:46 EST 1996

We are using the pGEX 2T and 3X vectors.  However, we cannot get the protein
off of the resin.  Any suggestions?  We have tried high glutathione and NaCl.
Thanks,  John Fellers, Univ of Kentucky

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