Taq Extender--degrading A overhangs!

Margon Vandongen vando006 at mc.duke.edu
Sat Mar 23 10:30:57 EST 1996


Jeffrey,

We had the same problem with PFU. I posted this a little while back:
spin your sample through a qiaex column to remove buffer and primers,
add taq buffer, dntp's, taq polymerase, put at 72 degrees for ten 
minutes and you are ready to ligate. Good luck,

Margon





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