are introns neccessary for transgene expression

milstone milstone at rascal.med.harvard.edu
Mon Mar 25 17:42:01 EST 1996


>Does anybody know if the presence of an intron makes a difference in the level of transgene expression?

Introns can improve expression. They may not always do so. Like most things with Tg animals, you will have to try it with your favorite transgene to know what will happen in your case. If you have good expression already, why worry?

Two refs (I suspect that there are several more) on introns in Tgs:

PNAS (1991)88:478-482.

Mol. Cell. Biol. (1991)11:3070-3074.




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