Northern blots without formaldehyde

Amie Franklin amief
Tue Mar 26 00:58:45 EST 1996


I have gotten great results with low formaldehyde RNA gels. The details,
running buffer 1x MOPS, RNA sample is in 1x MOPS,6.5% formaldehyde, 50%
formamide, 40microgram/ml EtBr, with bromophenol blue marker dye, heat 60c
2min, cool, load. The gel is 1.2-2% agarose 1xMOPS and 3ml of 37.5%formaldehyde
(obviously add after melting agarose). You still need to run this in a hood,
but its a lot less toxic.

good luck,
amief at candelab.berkeley.edu




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