Detection of molecular weight marker bands on +tively charged membranes
info at prozyme.com
Mon Mar 25 20:15:30 EST 1996
> We are currently performing a lot of Northern blotting using
> the glyoxal protocol and are looking for some way of detecting
> all bands of our molecular weight marker after electro-transfer.
> We wanted to use ethidium bromide but this reagent is incompatible
> with glyoxal, can anyone suggest another staining reagent or
> protocol that works well with glyoxal. Thanks Karin.
Acridine orange works well (soak the gel in 30 micrograms/ml). It isn't
quite as sensitive as ethidium bromide, and may require a different
camera filter. I last used it years ago and can't remember. I do
remember, though, that single-strand and double-strand nucleic acids
stain differentially. I presume you also glyoxalate your markers, so
that wouldn't be an issue.
bamsden at prozyme.com
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