porin production in E. coli - advice needed

Dilip Dias MAD2631 at rsgis6.tamu.edu
Mon Mar 25 14:40:22 EST 1996


JEAN
I AM NOT MUCH OF AN EXPERT ON PROTEIN EXPRESSION BUT HAVE DONE SOME 
EXPRESION USING pET vectors.  IF YOUR PROTEIN IS TOXIC THEN USE A HOST 
WITH STRINGENT INDUCTION CONTROL SUCH AS BL21DE3 pLYS S and a vector 
with an inducible promoter such pLAC. This would give you 2 levels of 
control. First the promoter (IPTG induced) second the pLYS S that binds 
to the T7 RNA polymerase if it is made in small quantities. Hope this 
helps.
Dilip





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