glycogen as a carrier
jpcd0 at mole.bio.cam.ac.uk
Tue Mar 26 06:42:44 EST 1996
In article <31576FB1.863 at fungus.biochem.ualberta.ca>,
colin at fungus.biochem.ualberta.ca wrote:
> Ari Kahn wrote:
> > I found a protocol for extracting DNA from low melting agarose at
> > http://research.nwfsc.noaa.gov/protocols.html In step 6 it asks for 1 ul.
> > glycogen as a carrier. What does this do for the pptn of the DNA? Is it
> > really necessary or will the pptn work without it?
> The 1ul is probably 1ul of a 20mg/ml solution (i.e. 20ug). Glycogen in
> precipitates much like DNA and at this amount forms a visible pellet.
> precipitate also likely traps the DNA in solution as well. The advantage of
> glycogen is that it will not affect subsequent reactions.
However our automated sequencing guys tell me that glycogen messes up
their specs when they try to quantitate the DNA. Does anyone know aneasy
way to get rid of it again? Apart from that it works a treat for me.
John Dixon Lab 44 (1223) 334131
Wellcome/CRC Institute Fax 44 (1223) 334134
Department of Genetics
United Kingdom e-m: jpcd0 at mole.bio.cam.ac.uk
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