DMSO and bacteriophage

john brennand john.brennand at gbapr.zeneca.com
Tue Mar 26 11:18:30 EST 1996


Helen

Assuming you have >10 7 individual plaques, I would recommend storing 
the libraries in 10 aliquots.  9 at -70 in dmso and 1 at 4o (+ CHCl3) as 
your working stock.  When that is used up, thaw another aliquot.

Having said that, I have freeze-thawed such libraries 2 or 3 times 
without detectable loss of titre.

John





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