Linear acrylamide (was --Re: glycogen as a carrier)

Glen Shearer gshearer at whale.st.usm.edu
Wed Mar 27 14:32:34 EST 1996


In article <3155EC76.60F2 at swt.edu>, Ari Kahn <ak01706 at swt.edu> wrote:

> I found a protocol for extracting DNA from low melting agarose at 
> http://research.nwfsc.noaa.gov/protocols.html.  In step 6 it asks for 1 ul. 
> glycogen as a carrier.  What does this do for the pptn of the DNA?  Is it 
> really necessary or will the pptn work without it?
> 
> ---protocol snipped------

How about using linear acrylamide as a carrier instead?
I've heard that it works well as a carrier, is easily made and does not
inhibit later reactions.

Any comments



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