EcoRI adaptors

James R. Miller jrm at lilly.com
Fri Mar 29 10:22:32 EST 1996


In article <1996Mar28.113758.8004 at inet.d48.lilly.com>, "Jane Q. Employee"
<Employee_Q_Jane at lilly.com> wrote:

> Has anyone had any experiance working with adaptors to adapt blunt ended 
> dna to a sticky end?  It seems as though they are easier to handle than 
> linkers in which you have to methylate your insert and then digest.  
> Linkers seem to be used more than adaptors, is that a real 
> observation??? Any tips on adaptor use? Pitfalls?
> 
> Thanks, from a first time user...

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