EcoRI adaptors

john brennand john.brennand at gbapr.zeneca.com
Fri Mar 29 09:38:47 EST 1996


Jane

Adaptors are the way to go - they work fine.

Only thing is, you need to be careful about getting rid of the excess 
dimerised adaptors.  If you don't all you will get will be adaptor 
clones - I speak from having fallen into that trap when making cDNA 
libraries.

So put your adapted DNA through a sizing spun column and then isolate 
the DNA from a gel (or put through a second sizing column). 2 steps are 
essential.

John





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