GST Fusion stuck on sepharose
Simon.Dawson at nott.ac.uk
Tue Mar 26 12:33:37 EST 1996
> We are using the pGEX 2T and 3X vectors. However, we cannot get the protein
> off of the resin. Any suggestions? We have tried high glutathione and NaCl.
Not sure that I remember it right but we had a guy here with the same
problem. I _think_ he said he adjusted the pH and that fixed it. Not being
familiar with the pGEX system, I don't know whether he raised or lowered it.
Still, it's somewhere to start. Hope thats of some help,
Dr. Simon Dawson TEL:+44 (0)115 9249924 ex. 44787
Dept. of Biochemistry FAX:+44 (0)115 9422225
Queens Medical Centre Email:Simon.Dawson at .nott.ac.uk
Clifton Boulevard http://www.ccc.nottingham.ac.uk/~mbzspd/Simon.html
U.K. "Back off man, I'm a scientist!" - Bill Murray.
More information about the Methods