glycogen as a carrier
Simon.Dawson at nott.ac.uk
Thu Mar 28 07:55:12 EST 1996
Ari Kahn wrote:
> I found a protocol for extracting DNA from low melting agarose at
> http://research.nwfsc.noaa.gov/protocols.html In step 6 it asks for 1 ul.
> glycogen as a carrier. What does this do for the pptn of the DNA? Is it
> really necessary or will the pptn work without it?
Well, unless I'm mistaken, the only thing the glycogen does is act as a
carrier molecule to facillitate the efficient precipitation of your DNA.
Carrier molecules make a significant difference if the concentration of your
DNA is fairly low.
Dr. Simon Dawson TEL:+44 (0)115 9249924 ex. 44787
Dept. of Biochemistry FAX:+44 (0)115 9422225
Queens Medical Centre Email:Simon.Dawson at .nott.ac.uk
Clifton Boulevard http://www.ccc.nottingham.ac.uk/~mbzspd/Simon.html
U.K. "Back off man, I'm a scientist!" - Bill Murray.
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