Methods March 1996 Archives by author

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Starting: Fri Mar 1 01:28:18 EST 1996
Ending: Sun Mar 31 23:35:03 EST 1996
Messages: 1286

Lipase assay "Marianne Leverone ", BIO
P.aeruginosa PAO-1 "Marianne Leverone ", BIO
Free Sulphydryl detection "Marianne Leverone ", BIO
resending CBN info "Thro, Ann Marie ", 3701, by way of Prakash at acd.tusk.edu C. S. Prakash
Need to remove nucleic acids from nuclear extracts ##Sophie## ##Candon##
DNA precipitation? Gregory S. Buzard 'Buz'
5' flourescein labelling and ABI, anyone tried it
Protocol for RT-PCR in shrimp Sittiruk Roytrakul - SCBC - 3536459
Site directed mutagenesis kits -recommend? at
inhibition of NF-kappa B at rzmail.uni-erlangen.de
Green Fluorescent Protein A.Doherty
Green Fluorescent Protein A.Doherty
Mycoplasma detection kits, and mycoplasma effects A.Doherty
SpinBind A.S.
Q: pRK5 vector sequence? AB
How to set up ELISAs Yasuhito Abe
Looking for UV-DNA endonuclease Michael Adamson
Clontech Laboratories is a BIOSCI sponsor!! BIOSCI Administrator
LI-COR, Inc. sponsors BIOSCI! BIOSCI Administrator
The Nest Group, Inc. sponsors BIOSCI! BIOSCI Administrator
IMPORTANT - BIOSCI Fundraising Update! BIOSCI Administrator
Heat lability of DEPC Adrian.Philbey at smtpgwy.agric.nsw.gov.au
Why serum starve before fos-jun induction ? Adrian.Philbey at SMTPGWY.AGRIC.NSW.GOV.AU
mRNA preps Adrian.Philbey at SMTPGWY.AGRIC.NSW.GOV.AU
PCR from saliva? Adrian.Philbey at SMTPGWY.AGRIC.NSW.GOV.AU
Heat lability of DEPC Adrian.Philbey at smtpgwy.agric.nsw.gov.au
Boomerang DNA Amplification Web Page Kevin Ahern
Taq stability? Christoph Ahlers
SOS!--PCR restriction cut Ashok Aiyar
NEEDED: codon bias table for Pseudomonas Shamima Akhter
Teicoplanin antibiotic (where can I get it) Al
Need to find source of teicoplanin Al
Need to find source of teicoplanin Al
Acetylcholinesterase (AChE) regeneration after DFP? Alan
Differential Display PCRs - help needed! Louis Alarcon
Prok. expression vectors; anything that is not based on Lac operon and Amp R? Mikhail Alexeyev
ISO Plasmid Not1 & Mlu1 Sites Mikhail Alexeyev
Prok. expression vectors; anything that is not based on Lac operon and Amp R? Mikhail Alexeyev
Purifying clean plasmid vectors for ligation Mikhail Alexeyev
tips for blunt ended ligation! Mikhail Alexeyev
How stable biotin-avidin? Mikhail Alexeyev
Tn5-GM Mikhail Alexeyev
elimination of cross reaction in polyclonal antiserum David P Alt
RNAzolB...Still Available? Eric C. Anderson
influenza hemagglutinin abs Thomas R. Anderson
Positive control for PTK probe Tracy E. Anderson
Culture of E.Coli (SURE) / Large Plasmids / Foreign Sequences Dr Alexander J Annala
Gibco's WWW site? Annette
PFGE references? Anonymous
Sources of baculovirus expression systems Tracy Aquilla
Anyone successfully doing RNAse protection Assays? Tracy Aquilla
Heat lability of DEPC Tracy Aquilla
NdeI: cutting problems?? anyone else?? seems silly! (fwd) Arioch
how to get rid of ethidium bromide??? Arioch
storing gels... Arioch
semi-dry elecrophoretic xfer Vs wet e.phoretic xfer ... Arioch
ß-Amyloid peptide solubility Joaquin Arribas
Ribozymes Bradley A. Arrick
cracked gels? Curt Ashendel
pHOOK (CaptureTec system) Graham Atherton
Immunohistochemistry: Technique Question Auditorium
vectors poly (A) Jo=?iso-8859-1?Q?=EBl BAGUET
baculovirus co-infection BIOTEC54
Response to "Quick Protein Concentration Assays" BOYDSTON at MVAXGR.HUNTER.CUNY.EDU
Staining DNA in Agarose with Methylene Blue Joseph C. Bagshaw
PCR probes by incorporation of 32P ? Joseph C. Bagshaw
Cycle Sequencing Joseph C. Bagshaw
single band from rRNA from brachionus sp. Joseph C. Bagshaw
Shrimp virus assay Joseph C. Bagshaw
DNA base composition Joseph C. Bagshaw
Northern blot and NybondN+membrane Joël Baguet
Gibco's WWW site? Roger Baird
Gibco's WWW site? Roger Baird
Help-no plasmid!!! Tim Barnett
Getting cells off plates L. Bastian
Transfection of HL60 L. Bastian
used equipment Michael Bausher
Lignin Enzymes wanted Michael Bausher
Rainin edp plus Michael Bausher
SDS in western blot transfert Michael Bausher
TA cloning Jose M. Bautista
Protein purification Jose M. Bautista
Structural Biochemistry Jose M. Bautista
Lambda exonuclease Robin Beech
Polymerases, anyone!?!?!? Brett Beitzel
Re- Touchdown PCR Protocol Brett Beitzel
Pyrimidine Dimers Brett Beitzel
RE- PCR Contamination Brett Beitzel
RE- Touchdown PCR Brett Beitzel
Re- Isolation of small DNA Brett Beitzel
Re- PCR contamination Brett Beitzel
Re- pZErO Brett Beitzel
inducible eukaryotic expression Michail Belyavskyi
large-scale prep of ssDNA Michael Benedik
Bmh71-18 strain Michael Benedik
Light Sensitivity of Tris Per Bergman
Sequencing Gel Rigs for SSCP Bernhard
SDS in western blot transfert Lionel Berthoux
Protocol for Re-using of Qiagen tips Trevor Bezdek
Protocol for Re-using of Qiagen tips Trevor Bezdek
Native PAGE @ pH < 6.5 Christopher Walentas Bioc
Tn5-GM Neil Crickmore Bioc
Peptides Darren Boehning
cracked gels? Darren Boehning
pAX Fusion Protein Vectors Torsten Boerchers
buy GP120 peptide? Russ Boggs
HELP : Stripping membranes with biotinylated proteins Patrice Boissy
HELP : Dosage of biotinylated proteins Patrice Boissy
transcription factor binding sites software? Eckhard Boles
Site directed mutagenesis kits -recommend? Eckhard Boles
Magnetic RNA isolation Reese Bolinger
5' RACE Peta C. Bonham-Smith
Why serum starve before fos-jun induction ? Avirup Bose
Unspecific hybridization to plasmid-vector Ian A. Boussy
Anyone using the ABI Catalyst 800 for genotyping? Don Bowden
Announcement: 1996 Cold Spring Harbor Lab Manual Source Book nw available Joan Boyce
influenza hemagglutinin abs Chris Boyd
tips for blunt ended ligation! Chris Boyd
GAPDH mRNA half-life Peter G Bradford
MICROSEQUENCING N-BLOCKED PROTEINS Andy Brauer
PCR stutters through oligo-A regions Jeffrey D. Bray
Primer Extension Sequencing Steven Bressler
calyculin A Annick Breton
ANNOUNCE: NAOMI Upgrade Simon M. Brocklehurst
Zonal gradients Juergen Brosius
rodent DNA and/or brains Juergen Brosius
Pumps for Zonal Rotors Juergen Brosius
PCR ampl. of a variable locus Juergen Brosius
PCR stutters through oligo-A regions Juergen Brosius
PCR stutters through oligo-A regions Juergen Brosius
PCR stutters through oligo-A regions Juergen Brosius
Boomerang, Vector-Splinker ette: Any success? Juergen Brosius
free sequence homology search on WWW Juergen Brosius
transformation problems Dr. Paul N. Brothers
Site directed mutagenesis kits -recommend? Dr. Paul N. Brothers
Media Question: What is Standard-1 from E. Merck? Paul N. Brothers
streptolysin experts wanted Darren D. Browning
Primer design (Oligo) Bill Burnett
RNA extraction-whole blood Neil Burns
Glycoprotein Detection Neil Burns
PCR contamination Neil Burns
Deuterium oxide in density gradient? Dr E. Buxbaum
Zonal gradients Dr E. Buxbaum
Immuno(histo)fluorescence: what's the best method? Dr E. Buxbaum
Isolation of nulcei Dr E. Buxbaum
cracked gels? Dr E. Buxbaum
mouse Raf genomic clones and sequence wanted Gregory Buzard
HELP! looking for N-ras intronic sequence. Gregory Buzard
PCR-SSCP ON ABI 373 WITH GENESCAN 672 Gregory Buzard
Clone Fingerprinting --SSCP Gregory Buzard
Polyacrylamide gel fixation for DNA Gregory Buzard
Polyacrylamide gel fixation for DNA Gregory Buzard
Polyacrylamide gel fixation for DNA Gregory Buzard
Amersham Hybond N problem C.L.L.
SOS!--PCR restriction cut C.L.L.
Anyone successfully doing RNAse protection Assays? CAShug
Salmon sperm DNA preparation CAShug
transformation problems CAShug
RNase protections assays. Too much salt? CAShug
Help: Ribonuclease protection assays. CAShug
isolation of small DNA fragments CDMENU at ccs1.cc.monash.edu.au
Wizard PCR purification from agarose gel CLAREN321
Unspecific hybridization to plasmid-vector Manuel G. CLAROS
(no subject) Bertrand COLLET
REAP Bertrand COLLET
Magnetic RNA isolation CPGYIELD
How stable biotin-avidin? CPGYIELD
low recovery with PromegaPolyATtract kit ? CPGYIELD
cDNA library..help CRH
p53's apoptotic function CSJGH at leeds.ac.uk
PCR Protocol for Mycoplasma Detection??? Carolyn Calnan
Scintillation Counting Without Scintillation Fluid Philip P Calvert
Differential Display PCRs - help needed! Miss J.M. Cameron
resolution on SDS-PAGE From Jaume Canaves
Trace Acetylation Protocol From Jaume Canaves
Protocol for RT-PCR in shrimp Tim F. Carl
(no subject) Rick Carroll
RNA removal/quartz plate Rick Carroll
plasmid cDNA library amplification CartonKJ
Primer Design Ed Castro
Primer Design sites Ed Castro
Where can obtain AMVN .... Depto. Biologia Celular
PtdIns(3,4,5)P3 Where do I get it? George W Chacko
Very pure total RNA free of DNA? Agnes S. Chan
phage display using Stratagene's Surfzap Kit Alex Chang
phage display using Stratagene's Surfzap Kit Alex Chang
Recombinant phage antibody system Chao-Liang Wu, PhD
Ps fluorescens biovars? Don Chen
In situ hybridization Gabriel Cheung
Help: Ribonuclease protection assays. Married with Children
silver stain LAU Tze Chin
Transfection of proteins: HOW? Nikolai Chitaev
<a href="http://www.bio.net/hypermail/sponsor.html"><img src="http://www.bio.net/hypermail/sponsor.gif"></a><br>bionet.molbio.methds-reagnts archives: HELP!---Immunoaffinity column doesn't work Nikolai Chitaev
Expression of GST fusion Gary T. Chou
longggg mRNA transcript Konstantin M. Chumakov
Extra Long PCR reamplification Konstantin M. Chumakov
A Budded Yeast: One or Two? Namjin Chung
Yeast Knockout? Namjin Chung
Radiolabelled phosphoinositols Jon Church
rare cutters 10-12 bp exist? Dr. Duncan Clark
TA cloning nightmare Dr. Duncan Clark
Prok. expression vectors; anything that is not based on Lac operon and Amp R? Dr. Duncan Clark
Taq stability? Dr. Duncan Clark
NH4Ac/EtOH ppt of DNA from PCR ? Dr. Duncan Clark
PCR through GC-rich Dr. Duncan Clark
NdeI: cutting problems?? anyone else?? seems silly! Dr. Duncan Clark
The KlenTaq mystery Dr. Duncan Clark
Artifact in plasmid preps Dr. Duncan Clark
Purifying clean plasmid vectors for ligation Dr. Duncan Clark
SOS!--PCR restriction cut Dr. Duncan Clark
Green Fluorescent Protein Dr. Duncan Clark
pZErO plasmid George Clark
Site directed mutagenesis kits -recommend? Sonya Clark
Immunohistochemistry: Technique Question Jerry Clayton
PCR in Nepal Dr. J.P. Clewley
Alkaline Phosphatase substrates Pierce Chemical Co
How stable biotin-avidin? Tehcnical Assistance / Pierce Chemical Co
ELISA reagent supplier help needed! Tehcnical Assistance / Pierce Chemical Co
Hydropathy plot program Wayne Coco
Virtual Reality in SYBR Space Wayne Coco
Hoeffer Progenetor II electroporation unit Robert Coelen
GeneClean II NaI conc??? Shelley Cole
RNA isolation- the long and the short of it... Shelley Cole
Wanted: Peptide quantitation method W.H. Colledge
Taq stability? Nicola Collie
RNA mass ladder greater than 10kb Martin Collinson
fax of SCHOTT in Germany or in France Pierre Commercon
fluorescently labelled protein Computer
How to gel PEG solutions? Terry Conley
antibodies to tetM Laura Conway
gal4 strains for yeast two-hybrid Seth Corey
pAX Fusion Protein Vectors Joao Vasconcelos Costa
Touchdown PCR Joao Vasconcelos Costa
Opinions on vaccuum centrifuge Andrea Crampton
Peptide synthesis Iain Crowder
mitochondrial DNA database Jim Cummins
PCR Primers Database - UPDATE. Jim Cummins
Clone fingerprinting Mr. A.R.J. Curtis
Clone fingerprinting Mr. A.R.J. Curtis
ISO Plasmid Not1 & Mlu1 Sites DNAGuy
ISO Plasmid with Not1 & Mlu1 Site DNAGuy
Polyacrylamide gel fixation for DNA DNAGuy
NH4Ac/EtOH ppt of DNA from PCR ? DSeegert
Store DNA concentrated or dilute? Richard Daines
pZErO plasmid Richard Daines
Cutting Qiagen miniprebs?? Richard Daines
What is "Cleavase" Raymond Dalgleish
C127I cells & 6-TG Ashraf R. Dallol
Thymidine kinase antibody Ashraf R. Dallol
sequencing murine p53 (fwd) Ashraf R. Dallol
Antibodies required..badly! Ashraf R. Dallol
rat cardiomyocyte cell line, who has one? Daniel
Want: Simple sequencing gel reader Phil Davey
COS-cells David L. Haviland, Ph.D.
Free Sulphydryl detection Michael Daws
Anyone heard of pCMV7? Michael Daws
Stripping Streptavidin-HRP Michael Daws
Stripping Streptavidin-HRP Michael Daws
E. coli sec mutants Elliott Dawson
Very pure total RNA free of DNA? Simon Dawson
Casein antibodies Simon Dawson
Opinion poll: Clonetech Matchmaker libraries Simon Dawson
Antibodies against inclusion body proteins? Simon Dawson
Gene clean reagent list. Simon Dawson
GST Fusion stuck on sepharose Simon Dawson
glycogen as a carrier Simon Dawson
SpinBind Oliver S. DePeyer
SpinBind Oliver S. DePeyer
SpinBind Oliver S. DePeyer
SpinBind Oliver S. DePeyer
SpinBind and Supelco Oliver S. DePeyer
differential display Harm Deckers
mRNA Differential Display Harm Deckers
Alpha Satellite Probes for Mice??? Where can you get them? FOR FISH Graham Dellaire
Distinct bands in restriction dig. of genomic DNA Graham Dellaire
Announce WWW: Analytical Ultracentrifugation/XL-A Borries Demeler
HELP! GST column with Tris-Saline? Michael DiDonato
SOS Call to all experinced "Sequencers" Michael DiDonato
TA cloning nightmare Catherine I. DiOrio
Qiaex problems Catherine I. DiOrio
low recovery with PromegaPolyATtract kit ? Catherine I. DiOrio
tips for blunt ended ligation! Dilip Dias
tips for blunt ended ligation! Dilip Dias
porin production in E. coli - advice needed Dilip Dias
porin production in E. coli - advice needed Dilip Dias
porin production in E. coli - advice needed Dilip Dias
RXR antibodies Eddie Diehl
Storage of serum in frost-free freezers? Eddie Diehl
tips for blunt ended ligation! Eddie Diehl
Storage of serum in frost-free freezers? Eddie Diehl
cracked gels? Eddie Diehl
Any mass spec for protein sequencing under $30,000? Eddie Diehl
Carb vs Amp - whats the story? John Dixon
What size DNA stays in soln when NH4AC ppt? John Dixon
glycogen as a carrier John Dixon
Expand PCR and TA cloning kits? John Dixon
superbroth protocol finally finished Guivarc_h Dominic
Qiaex problems Lynne M Donegan
Plasmid Drawing Programme Tom Dougherty
Northern blot and NybondN+membrane Gerald Downes
A question about in situ hybridization Martin Downes
Can dessicants be dried in microwave? Mark Dowton
ELISA reagent supplier help needed! Dr.G.R.Rajasekariah
ELISA reagent supplier help needed! Dr.G.R.Rajasekariah
Microplate technology Dr._Jose_Romero_at_WRAIR3__WASHDC at WRSMTP-CCMAIL.ARMY.MIL
Streptavidin in purification by affinity Dr._Jose_Romero_at_WRAIR3__WASHDC at WRSMTP-CCMAIL.ARMY.MIL
Has anyone done NF1 (The transcription factor) Northerns? EILEEN
Guinea Pig B-actin seq. Help! Mr N.C. Eastmond
Spectrophotometer Cuvettes Mr N.C. Eastmond
Spectrophotometer Cuvettes Mr N.C. Eastmond
PCR method Andreas Ebrecht
What is Touchdown PCR? Craig Echt
Expression of GST fusion John Elliott
Expression vector tags John Elliott
Expression vector tags John Elliott
messy agarose bands John Elliott
info on HPLC and related techniques John Elliott
EcoRI adaptors Jane Q. Employee
Is B-Gal phosphorylated in mammalian cells? Steven Enkemann
1500+ scientists obtain free BCMD subscriptions - bcmd.msg [1/1] Ernest Beutler, M.D.
1500+ scientists obtain free BCMD subscriptions - bcmd.msg [1/1] Ernest Beutler, M.D.
req. opinions of Ericomp thermocycler Brad Fabbri
Gibco Site Patrick HJ Falckh
RNA isolation/Chomozynski Patrick HJ Falckh
subscribe Don Faller
PCR can not get intense bands Zahra Fayazi
PCR can not get intense bands Zahra Fayazi
Protocol for Re-using of Qiagen tips Iain Fenton
Superbroth for EVERYONE! (NOT) elizabeth Ferguson
Transfection of proteins into cells: HOW? elizabeth Ferguson
Use of d(AT)9 probe for detection of microsatellites Marcel Fernandez
anti dsDNA latex agglutination Rafel Ferragut
Opinions on CapFinder? John Finnerty
PCR amplification of mouse kappa chains H. David Fischer
Large scale low melt extraction Karl Fischer
Salmon sperm DNA preparation Karl Fischer
Bmh71-18 strain Karl Fischer
Expression of GST fusion L. Fliegel
chickens vs rabbits for antibodies L. Fliegel
Green Fluorescent Protein L. Fliegel
Vascular Endothelial and Smooth Muscle Cells John Fox
Getting cells off plates John Fox
chickens vs rabbits for antibodies Richard Van Frank
Alkaline gels-Better method? Amie Franklin
nicking DNA plasmids on purpose Amie Franklin
BioRad GeneLite Kit Amie Franklin
TA cloning nightmare Amie Franklin
Lignin Enzymes wanted Amie Franklin
Very pure total RNA free of DNA? Amie Franklin
Purifying clean plasmid vectors for ligation Amie Franklin
Northern blots without formaldehyde Amie Franklin
Luminimetry with luciferase: wavelength please! I.R. Franklin
Inoue Competent Cell procedure Richard Friedman
Density gradient cell separation and streptavidin-agarose? G.P.
Non-detergent containing lysis buffer for primary cell culture? G.P.
Differential centrifugation of cells and streptavidin-agarose? G.P.
Gibco's WWW site? G.P.
SYBR Reply STEVEN GERHARDT
thrombin source for fusion prot cleav. GLUXLAB
Touchdown PCR protocoll? Olivier Gandrillon
Protein purification Inmaculada Garcia
NH4Ac/EtOH ppt of DNA from PCR ? Royal Botanic Gardens
How stable biotin-avidin? Michael Giffin
in-situ hyb in lung: method of fixation? Gil
Solaris (Unix) on IntelPC Don Gilbert
freeze-squeeze Michael Gillings
Very pure total RNA free of DNA? Michael Gillings
Plant Expression Vectors Dr. Randal W. Giroux
Purifying clean plasmid vectors for ligation Steven Goldberg
Heterologous probe a problem in Southern Steven Goldberg
Info about Auto Sequencer...! Steven Goldberg
Purifying clean plasmid vectors for ligation Steven Goldberg
Re- pZErO Steven Goldberg
PCR: How to remove DNA contamination from the starting mRNA? Simon Golding
Green Fluorescent Protein Purification Short Courses Daniel Gonzalez
southern blotting of small fragments Liam E Good
semi-blotting Liam E Good
Sequencing after G run Michael Gorry
Mitochondrial DNA extraction? Anita Gould
Sequence of Lambda DASH II polylinker Anton Scott Goustin
Magnetic RNA isolation: do-it-yourself beads Anton Scott Goustin
Linking an oligonucleotide to an antibody Anton Scott Goustin
Spectrophotometric estimation of RNA Anton Scott Goustin
RT of G-cap into C? Anton Scott Goustin
LexA antibodies? Ian Graham
RNA extraction-whole blood Ian Graham
Linear acrylamide (was --Re: glycogen as a carrier) David Grant
Transforming bacteria for protein production Patrick Grealish
Is there a FAQ for this group? Patrick Grealish
Transfection of proteins into cells: HOW? Brian T. Greuel
Help-no plasmid!!! Mark Griffiths
large-scale prep of ssDNA Duke Groebe
Store DNA concentrated or dilute? Duke Groebe
Store DNA concentrated or dilute? Duke Groebe
isolation of small DNA fragments Duke Groebe
DNA binding Protein Inhibition Duke Groebe
ALPHA ANNOUNCES NEW PROCEDURES TO ENHANCE PLASMA PRODUCT SAFETY John Gross
Getting cells off plates Mike Gruidl
removal of starch (fwd) Baozhu Guo
Light Sensitivity of Tris Ferland Louis H.
Peptide SLIGRL Ferland Louis H.
Protocol for Re-using of Qiagen tips Ferland Louis H.
RNA isolation/Chomozynski Ferland Louis H.
Protocol for Re-using of Qiagen tips Ferland Louis H.
Help needed for DNase I Footprinting Ferland Louis H.
Looking for a histochemical ATPase protocol... H.Hoppeler
Looking for a histochemical ATPase protocol... H.Hoppeler
RT efficiency Michael HERMAN
TNT kit with T7 RNA Pol HONG
COS-cells Allison Haggarty
anti-HA cross reaction? Allison Haggarty
DNA binding Protein Inhibition Tina Hallis
fluorescently labelled protein Richard R. Hardy
NdeI: cutting problems?? anyone else?? seems silly! (fwd) Neil Harris
Shading Functional Domains Neil Harris
Help: Lambda purification kits Ulrich Harttig
RT-PCR Burkhard Hassel
Inverse PCR Helen J. Hathaway, Ph.D.
Degenerate primers for PCR HelenCS
Anyone successfully doing RNAse protection Assays? Helmut
Can one gene be used to select for OR against.... Paul N Hengen
freeze-squeeze Paul N Hengen
Amersham Hybond N problem Paul N Hengen
Sequencing after G run Paul N Hengen
Leaky Mini-ProteanII unit. Paul N Hengen
How stable biotin-avidin? Paul N Hengen
Carb vs Amp - whats the story? Paul N Hengen
Large scale low melt extraction Paul N Hengen
Store DNA concentrated or dilute? Paul N Hengen
Artifact in plasmid preps Paul N Hengen
Methods and Reagents FAQ list Paul N Hengen
How stable biotin-avidin? Paul N Hengen
A question about in situ hybridization Paul N Hengen
Methylen blue DNA staining Paul N Hengen
Boomerang DNA Amplification Web Page Paul N Hengen
What is Touchdown PCR? Paul N Hengen
Touchdown PCR Paul N Hengen
Reply: DN Ligation Paul N Hengen
Primer extention analysis by PCR Paul N Hengen
Inoue competent cell procedure Paul N Hengen
Is there a FAQ for this group? Paul N Hengen
Pellet paint ? Paul N Hengen
Expression of GST fusion Bernard Heymann
Qiagen RNeasy: Early precipitate?? Jim Hill
Green Fluorescent Protein Kent Hill
Can dessicants be dried in microwave? Mark Hill
GeneClean II NaI conc??? Walter Hill
Pellet paint ? Walter Hill
R.E.D. problems Andrew Hniat
anti 5-lipoxygenase antibodies Dr. Stephen Hoare
Protein inhibitor(s) during immunoprecipitation ? Dr. Stephen Hoare
Expression vector tags Jon Hobman
electroporation Marcel Hoffmann
tuning of expression levels Marcel Hoffmann
RNase protections assays. Too much salt? Angela Hofstra
Taq stability? Susan Jane Hogarth
Store DNA concentrated or dilute? Susan Jane Hogarth
Large scale low melt extraction Mike Holbrook
disrupting filimentous fungi Andrew Hopkins
G418 Henry Hoppe
HEX-labelled primer doesn't work in PCR ? Shirley Horn-Saban
Homemade shaking incubator Bob Horton
TA cloning nightmare Bob Horton
Taq stability? Bob Horton
REPOST Homemade Shaking Incubator Bob Horton
SOS!--PCR restriction cut Bob Horton
longggg mRNA transcript Bob Horton
transcription factor binding sites software? Bob Horton
PCR in Nepal Bob Horton
how to get rid of ethidium bromide??? Bob Horton
PCR probes by incorporation of 32P ? Robert Horton
Homemade Shaking Incubator - decode pictures Robert Horton
PCR contamination-HELP! David Hosfield
Help about blood storage please! Dave Hostler
Help about blood storage please! Dave Hostler
Primer extention analysis by PCR Ken Howe
Degenerate primers for PCR Mr. Chris Hoyle
Gibco's WWW site? Peter Hunziker
EtOH + PBS = precipitation? Jim Hutchins
Why use 3´to5´exo-negative DNA-Polymerases? Horst Ibelgaufts
what is a cytofectin? Horst Ibelgaufts
Gibco's WWW site? Horst Ibelgaufts
Gibco WWW site Horst Ibelgaufts
what is a Far South-Western blot? Horst Ibelgaufts
tips for blunt ended ligation! Kenneth Y. Ilio
anti-myristoylated glycine Ab Atsushi Ishikawa
low recovery with PromegaPolyATtract kit ? Toshiharu Ishizuka
messy agarose bands J.C.
Anyone have indepth info on annealing temps and unzipping temp or equation for PCR components? JCM
protoplast isolation JWU at LAN.TJHSST.EDU
(no subject) T. James
Wizard PCR purification from agarose gel Markku.M Jeltsch
Distinct bands in restriction dig. of genomic DNA Markku.M Jeltsch
Glutathione redox buffer? Csaba Jeney
Storage of serum in frost-free freezers? Jfloring at aol.com
What size DNA stays in soln when NH4AC ppt? Xiaohui Ji
Wanted: tk promoter - b gal construct Changcun Jia
ATTO supplier JoanBoyce at aol.com
How to get a free copy of the 1996 CSHL Source Book JoanBoyce at aol.com
GST Fusion stuck on sepharose John
Fog in In Vitro Translations John
Chemiluminescence, western blotting, problems... John K. Troyer, PhD.
Non-detergent containing lysis buffer for primary cell culture? John K. Troyer, PhD.
Modified pGEM? S John
Ethidium bromide stained acrylamide gels for microsatellites Rick Johns
Polyacrylamide gel fixation for DNA David R. Johnson
Northern blots without formaldehyde Doug Johnson
single band from rRNA from brachionus sp. (Dave Johnston)
NEEDED: codon bias table for Pseudomonas Robert Johnston
Biological Data Transport info R. Scott Jokerst
Plasmid Drawing Programs - where to find... R. Scott Jokerst
Peptide Nucleic Acids (PNA) Mick Jones
nicking DNA plasmids on purpose Zophonias O. Jonsson
How stable biotin-avidin? Zophonias O. Jonsson
What's behind the AmpliTaq Gold trick? Zophonias O. Jonsson
Can dessicants be dried in microwave? David Joyce
Transforming bacteria for protein production Vanhauwe Jurgen
rare cutters 10-12 bp exist? Gary K.
ISO Plasmid with Not1 & Mlu1 Site Gary K.
need Serva contact info Gary K.
GeneClean II NaI conc??? Gary K.
freeze-squeeze Dr. K.Hoare
Large scale low melt extraction K.James
PCR can not get intense bands KHUANG at CHEMVX.TAMU.EDU
Phage DNA Isolation KHUANG at CHEMVX.TAMU.EDU
RNA isolation DANIEL Y KIM
Anyone heard of pCMV7? KPH
HRPO linking to oligos? Chris Kafer
Microinjection: where to find info about? Stefan Kahlert
Transfection of proteins into cells: HOW? Stefan Kahlert
Transfection of proteins into cells: HOW? Stefan Kahlert
glycogen as a carrier Ari Kahn
pZErO plasmid Arnoud Kal
suitable loading controls for RPA's in diabetic vs. normal rats Nick Kallincos
Reagent Availabilty Michael Kane
Ab to inositol monophosphatase Michael Kane
EtBr optimal emission spectra Torsten Karlsson
pHOOK (CaptureTec system) Nigel Kenward
freeze-squeeze Nigel Kenward
Transfection of proteins into cells: HOW? Nigel Kenward
Alkaline Phosphatase substrates Gabriele Kerber
Siliconized coverslips for In Situ Hybridization Gabriele Kerber
NdeI: cutting problems?? anyone else?? seems silly! (fwd) Rich Kernan
Screening for DNA binding protein Rich Kernan
Any commercially available plasmids with HA, myc or T antigen tags. Obaid Y. Khan
Site-directed PCR mutagenesis Hans Kiefer
Help: looking for a source for phytochelatin. Jin-Hong Kim
Internalization of the protein into cultured cells Seong-Hun Kim
electroporation of Streptomyces sp. Dima Klenchin
Superbroth for EVERYONE! (NOT) Dima Klenchin
Leaky Mini-ProteanII unit. Dima Klenchin
Transfection of proteins into cells: HOW? Dima Klenchin
need Serva contact info Dima Klenchin
How stable biotin-avidin? Dima Klenchin
disrupting filimentous fungi Dima Klenchin
cracked gels? Dima Klenchin
SDS in western blot transfert Dima Klenchin
Getting cells off plates Dima Klenchin
source for ganciclovir Dima Klenchin
mouse lymphotoxin David Knight
PCR hood Bob Kodrzycki
purification of 140 Kbp from agarose Riekeltje Koedood
freezer purchase Riekeltje Koedood
COS-cells Riekeltje Koedood
Leaky Mini-ProteanII unit. Riekeltje Koedood
Salmon sperm DNA preparation Riekeltje Koedood
What size DNA stays in soln when NH4AC ppt? Riekeltje Koedood
cracked gels? Riekeltje Koedood
PCR contamination-HELP! Riekeltje Koedood
chylomicron retention Karl G. Kohlgraf
cracked gels? Helen Korsmo
TOL Plasmid Prep - protocol K.G. Korth
Abbreviated miniprep protocol... Incredible! Alexander Kraev
Amersham Hybond N problem Alexander Kraev
Heat lability of DEPC Alexander Kraev
Cycle Sequencing Alexander Kraev
Salmon sperm DNA preparation Alexander Kraev
Large scale low melt extraction Alexander Kraev
Excision of LambdaZap Products Alexander Kraev
HELP! GST column with Tris-Saline? Cornelius Krasel
HELP! GST column with Tris-Saline? Cornelius Krasel
Reply: DN Ligation Andrea Krempler
info about NTCB Krishnaswamy
Can one gene be used to select for OR against.... Sanjay Krishnaswamy
Hefe-Klonierung Georg Kroeger
Transcription factors and transfections Karen Kroeger
Taq stability? Dubear Kroening
Adenovirus expression systems Valeri Krougliak
PCR contamination-HELP! Warren D. Kruger
test, please ignore Gary Kucera
NdeI: cutting problems?? anyone else?? seems silly! (fwd) Gary Kucera
small PCR product-->ligation Gary Kucera
Expression of GST fusion Petri Kursula
Alkaline Phosphatase substrates martin LEACH
FBS users PLEASE READ - IMPORTANT martin LEACH
FBS users PLEASE READ - IMPORTANT martin LEACH
Gibco's WWW site? martin LEACH
elisa plates??? LMordo
direct blotting system LMordo
Inoue competent cell procedure Rico Laage
Non-rad. Northerns Rico Laage
Chemiluminescence, western blotting, problems... Hedrick Lab
Prok. expression vectors; anything that is not based on Lac operon and Amp R? Hedrick Lab
Prok. expression vectors; anything that is not based on Lac operon and Amp R? Hedrick Lab
PCR contamination-HELP! Morrison Lab
A fastidious contaminant John Ladasky
20% gel drying Laigle
How stable biotin-avidin? Marc Lamphier
low recovery with PromegaPolyATtract kit ? Marc Lamphier
Help? Immunoprecipitation Marc Lamphier
Catalytically active purified Ras? Andrew Lange
Allelic Ladder-source needed? Garry P. Larson
Northern hybridizations Stephen R. Lasky
Store DNA concentrated or dilute? Stephen R. Lasky
oligo annealing/cloning problem Robert F. Leclerc
DH5 alpha David S. Lee
large-scale prep of ssDNA Yuandan Lee
PC12 cells Philippe Lefebvre
Site-directed PCR mutagenesis Philippe Lefebvre
Salmon sperm DNA preparation Rachelle Leger
mRNA preps Rachelle Leger
Polyacrylamide gel fixation for DNA Danny Leung
PCR through GC-rich Dale Levitzke
PCR through GC-rich Dale Levitzke
Re-post: PCR through GC-rich Dale Levitzke
Luciferase in Vero problems David N. Levy
Gel Analysis - DNA ProScan David Ley
Help: Rapid determination of copy No. of a gene in bacterial chromosome. Xiang Li
Acetylcholinesterase (AChE) regeneration after DFP? T. Chyau Liang
alpha-S dNTPs Michael Lichten
Making His-tag affinity resin Rita Lim
Want: Simple sequencing gel reader Bob Litt
Background in Southern Blots Jerry Litt
Tyramide Signal Amplification Jerry Litt
DMSO and bacteriophage Helen Logan
Best chemiluminescence for W.blotting Jen Logan
TNT kit with T7 RNA Pol Jen Logan
Getting cells off plates Jen Logan
yeast transformation efficiency Ray Lu
Self competition in EMSA Menglin Luo
Gibco's WWW site? R. John Lye
Storage of serum in frost-free freezers? R. John Lye
TNT kit with T7 RNA Pol Jennifer Lyon
How to gel PEG solutions? Ted M.
Pellet paint ? Ted M.
good sequencing unit Ted M.
Best chemiluminescence for W.blotting MALINI at JASON.UTHCT.EDU
PCR through GC-rich MAN!!!
Re-post: PCR through GC-rich MAN!!!
SpinBind MAN!!!
Thanks for tips for GC-rich PCR! MAN!!!
Commercial nonradioactive riboprobes Paul C. Grimm MD
Spectrophotometric estimation of RNA SRINIVAS MUMMIDI
POSITION AVAILABLE AT BIO-RAD LABORATORIES MacBoyee
Kan resistance and pH Rafael Maldonado
ANNOUNCE: tacg - Restriction Enzyme analysis tool for unix Harry Mangalam
are introns neccessary for transgene expression Liz Mann
nicking DNA plasmids on purpose Ned Mantei
Distinct bands in restriction dig. of genomic DNA Ned Mantei
Inoue Competent Cell procedure Ned Mantei
Ethidium bromide stained acrylamide gels for microsatellites Ned Mantei
Alternative to autoclaving for denucleasing plasticware Ned Mantei
Inoue protocol with reducing agents. And speedprep David Martin
Peptide synthesis David Martin
chickens vs rabbits for antibodies David Martin
Free Sulphydryl detection David Martin
Polymerases, anyone!?!?!? Martin_K_Burnham at SBPHRD.COM
Large scale low melt extraction J. Pat Martinez
substractive libraries: suggestions ?? Jean-Yves Masson
Does TK work in Neuro-2a cells? Matthew P. Frosch, M.D., Ph.D.
Looking for a histochemical ATPase protocol... Matthew P. Frosch, M.D., Ph.D.
influenza hemagglutinin abs Mauro
Aminopropanol mutant Jean-Francois Mayaux
Addendum: Sequencing Gel Rigs for SSCP Bernhard Mayr
Addendum: Sequencing Gel Rigs for SSCP Bernhard Mayr
Magnetic RNA isolation: do-it-yourself beads Mark McCormick
FloriGen newsgroup keyword search Bill McKendree
(Q) Problem w/silver enhancement of gold particles Kenneth J McNeil
single-strand DNA break detection Scott Selby Med-Hem
Non-rad. Northerns Scott Selby Med-Hem
Techniques to study genetic drift (query) Ravi Menon
TNT kit with T7 RNA Pol Kojo Mensa-Wilmot
Heat lability of DEPC P. Mertens
transformation problems P. Mertens
PCR-cloning P. Mertens
PCR can not get intense bands P. Mertens
Help about blood storage please! P. Mertens
Superbroth for EVERYONE! (was NOT, but now IS) Alex Merz
Differential centrifugation of cells and streptavidin-agarose? Mary Meza
Robot Cycler Ted Michelini
Degenerate primers for PCR Ted Michelini
The KlenTaq mystery Ted Michelini
How does pH effect Terrific Broth? Ted Michelini
Can dessicants be dried in microwave? Michelle.Gleeson at SMTPGWY.AGRIC.NSW.GOV.AU
Can dessicants be dried in microwave? Michelle.Gleeson at SMTPGWY.AGRIC.NSW.GOV.AU
Amersham Hybond N problems Michelle.Gleeson at SMTPGWY.AGRIC.NSW.GOV.AU
Reply: DN Ligation David Micklem
Dye and terminator chemistry Microplate at eworld.com
Microplate technology Microplate at eworld.com
elisa plates??? Microplate at eworld.com
Gibco's WWW site restriction on server? Microplate at eworld.com
Alternative to autoclaving for denucleasing plasticware Microplate at eworld.com
Getting cells off plates Microplate at eworld.com
Alternative/autoclaving for denucleasing plasticware STOP Microplate at eworld.com
Artifact in plasmid preps Mark R. Miglarese
Why serum starve before fos-jun induction ? Mark R. Miglarese
Protein inhibitor(s) during immunoprecipitation ? Mark R. Miglarese
Bmh71-18 strain Mark R. Miglarese
PCR: How to remove DNA contamination from the starting mRNA? Mark R. Miglarese
Site directed mutagenesis kits -recommend? Mark R. Miglarese
G418 Mike
COS-cells Mike
inducible eukaryotic expression Mike
Need help with Qiagen PB Buffer? Charles A Miller
How does pH effect Terrific Broth? Charles A Miller
EcoRI adaptors James R. Miller
Chemiluminescence, western blotting, problems... Giampaolo Minetti
Chemiluminescence, western blotting, problems... Giampaolo Minetti
Leaky Mini-ProteanII unit. Giampaolo Minetti
disrupting filimentous fungi William Moens
Inoue Competent Cell procedure Christian Moews
COS-cells Christian Moews
Transfection of lymphoid leukemia cell lines Richard Moldwin
Genescan and Diff Display Mona
enantiomeric amino acids Michael Moormann
Fog in In Vitro Translations Michael Morales
are introns neccessary for transgene expression Michael Morales
Northern controls John Morlan
Northern controls John Morlan
Northern controls John Morlan
Slow dye for sequencing gels? Stephen Morris
activated T-cell library for 2-hyb?? Heidi Moss
NdeI: cutting problems?? anyone else?? seems silly! Heidi Moss
human genomic library Markus Stefan Mueller
plasmid cDNA library amplification TJ Murphy
Can dessicants be dried in microwave? Bernard Murray
Chemiluminescence, western blotting, problems... Bernard Murray
Store DNA concentrated or dilute? Bernard Murray
SEAP Bernard Murray
Store DNA concentrated or dilute? Bernard Murray
NdeI: cutting problems?? anyone else?? seems silly! (fwd) Bernard Murray
Best chemiluminescence for W.blotting Bernard Murray
Protein inhibitor(s) during immunoprecipitation ? Bernard Murray
PCR can not get intense bands Bernard Murray
Testis library for two-Hybrid?? Jon Nakamoto
PCR probes by incorporation of 32P ? Geoffrey Neale
Home-made reverse transcriptase. Richard Near
Antisense gene knockout Richard Near
Mutagenesis kits Rick Neubig
transfection standard Soeren Jensby Nielsen
Protocol for Re-using of Qiagen tips Mads Noerregaard-Madsen
Casein antibodies Gustavo Nolasco
Custom Antibodies Gustavo Nolasco
Western blot mystery: update Pamela Norton
PCR stutters through oligo-A regions Pamela Norton
RNase protections assays. Too much salt? Pamela Norton
Reply: DN Ligation Pamela Norton
DNA binding Protein Inhibition Pamela Norton
free sequence homology search on WWW Jane O'Brien
inosine primers Tm?? Mr D.P. O'Hare
Meeting - H.pylori Ronan O'Kennedy
Wizard PCR purification from agarose gel Tae Gwon Oh
Transcription Factors Sergey V. Orlov
some information about hormones needed... Sergey V. Orlov
Ribonuclease Protection Assay (RPA) Osama
Trial sending Osama
PCR contamination Osama
RNase Protection Assay hint.. Osama
Plasmid Drawing Program MARK PEGGIE
transformation problems GILLIAN NI BHARLO BOTANY PG
TA cloning GILLIAN NI BHARLO BOTANY PG
In situ hybridisation GILLIAN NI BHARLO BOTANY PG
cDNA molecular wieghts GILLIAN NI BHARLO BOTANY PG
Northern hybridizations PRETORIUS
porin production in E. coli - advice needed Jean-Yves Paquet
storing gels... Monica L. Parker
Lignin Enzymes wanted Mick Partis
Robot Cycler Pascal_Bochet
cracked gels? Pascal_Bochet
RNA isolation Susan Patrick
RNA isolation/Chomozynski Susan Patrick
RNA isolation/Chomozynski Susan Patrick
RNA isolation/Chomozynski Susan Patrick
Anti-microbials Paul
Heat lability of DEPC Paul
GeneClean II NaI conc??? Chip Peele
RNA isolation/Chomozynski Daniel Pereira
oligo annealing/cloning problem Daniel Pereira
RNase protections assays. Too much salt? Daniel Pereira
seeking protocal for RNase assay by gel electrophoresis Peter
Blocked Xba I sites? Todd Miller - Pharmacology
Want: Simple sequencing gel reader Andy Phillips
Clone fingerprinting Andy Phillips
Looking for supplier Andy Phillips
cDNA library kits-your choice Andy Phillips
T4 ligase clone Scott Vande Pol
chickens vs rabbits for antibodies Scott Vande Pol
Thymidine Kinase (Repost) Polash
RT-PCR and cDNA synthesis from bacterial total RNA Oliver Politz
SfiI enzyme Andrei Popov
purification of 140 kbp from agarose Andrei Popov
purification of a 140 kb fragment Andrei Popov
PCR AMPLIFICATION OF MOUSE KAPPA CHAINS Andrei Popov
Does anyone have an antibody to HSVtk? Phil Potter
Biotech NewsReport C. S. Prakash
large-scale prep of ssDNA Kiley R. Prilliman
Robot Cycler Kiley R. Prilliman
SOS!--PCR restriction cut Kiley R. Prilliman
Expression vector tags Kiley R. Prilliman
TNT kit with T7 RNA Pol Hans Bernd Prisack
TNT kit with T7 RNA Pol Hans Bernd Prisack
Nuclease P1, Penicillium citrinum ProZyme
SOS!--PCR restriction cut ProZyme
cracked gels? ProZyme
Detection of molecular weight marker bands on +tively charged membranes ProZyme
rxn of protein a with denatured ab ProZyme
PCR: How to remove DNA contamination from the starting mRNA? QIAGEN
Qiagen RNeasy: Early precipitate?? QIAGEN
Need help with Qiagen PB Buffer? QIAGEN
Fragment prep. using QiaTip? QIAGEN
Protocol for Re-using of Qiagen tips QIAGEN
35S-RFLPs Javier Quinteiro
yeast transformation efficiency ZYMO RESEARCH
cDNA library kits-your choice RMMMS at CUNYVM.CUNY.EDU
Sources of baculovirus expression systems RMMMS at CUNYVM.CUNY.EDU
Expression vector tags RMMMS at CUNYVM.CUNY.EDU
Hsp70 Expression Vectors RMMMS at CUNYVM.CUNY.EDU
Leaky Mini-ProteanII unit. HIRANYA ROYCHOWDHURY
Agarose and preparative gels... HIRANYA ROYCHOWDHURY
Agarose and preparative gels... HIRANYA ROYCHOWDHURY
PCR can not get intense bands HIRANYA ROYCHOWDHURY
Drying sequencing gels HIRANYA ROYCHOWDHURY
Help about blood storage please! Jimmy Rail
Help with Transcription Factors Database? Jimmy Rail
Inoue competent cell procedure Arfaan Rampersaud
Superbroth for EVERYONE! (was NOT, but now IS) Robert Randolph
chickens vs rabbits for antibodies Robert Randolph
Wizard PCR purification from agarose gel Colin Rasmussen
glycogen as a carrier Colin Rasmussen
Plasmid Drawing Programme Soren K. Rasmussen
Drying sequencing gels John Rebers
Membrane properties Achim Recktenwald
Free Sulphydryl detection Achim Recktenwald
ANTI-RAT ANTIBODIES Chris Regan
2D-Software Harry Reis
cDNA library..help Andre Renard
Wizard maxipreps Surgical Residence
DNA Isolation Kit Surgical Residence
Chemiluminescence, western blotting, problems... Surgical Residence
ANNOUNCE: Primers! for the Mac v1.0 Richard J. Resnick
HELP! looking for N-ras intronic sequence. Erwin Pauws & Carrie Ris
SDS PAGE buffers cost saving Phillip Robinson
Cycle Sequencing Robmit
Mutagenesis kits Elizabeth Rogers
Wizard PCR purification from agarose gel Don Rollins
req. opinions of Ericomp thermocycler Don Rollins
Repetitive Northern Hybridization Gabriel Romero
GST Fusion stuck on sepharose David Roof
LCR (Ligase Chain Reaction) kit Abraham Rosenberg
Antibodies against dermatophytes Steffen Roth
Post-doc needed Jay L. Rothstein
Anyone have indepth info on annealing temps and unzipping temp or equation for PCR components? Eitan Rubin
freeze-squeeze Eitan Rubin
Anyone have indepth info on annealing temps and unzipping temp or equation for PCR components? Eitan Rubin
Distinct bands in restriction dig. of genomic DNA Eitan Rubin
Leaky Mini-ProteanII unit. George Rutherford
yeast transformation efficiency S.L. Rennie BSc, Lab Manager
How to put a phosphate group on 3'OH of ssDNA? Robert SCHROCK
Amersham Hybond N problem BRITTA SCHULZ
Magnetic RNA isolation M.W. SMITH
TA cloning nightmare Amna Saeed
The KlenTaq mystery Amna Saeed
Inoue Competent Cells Amna Saeed
PCR from saliva? Igor R Sagdeev
Membrane properties Michail P. Sahsamanoglou
pAX Fusion Protein Vectors Krish Sakhamuru
Post-doc needed Klaus Salger
Northern blots without formaldehyde Klaus Salger
Testis library for two-Hybrid?? Michele Sallese
expression vector tags Tony Sanchez
PCR on cells in suspension Tony Sanchez
PCR Lyme Disease (DNA extraction protocol from serum) Paulo Santos
SpinBind and Supelco Jasper Saris
TROPIX Dual-LIght P. Vijay Sarthy
(none) Hajime Sawada
Gene clean reagent list. Ron Sayler
rat cardiomyocyte cell line Frank Schaap
PALA only from NCI? Andreas Schaffer
5' RACE Steve Scherer
Ribonuclease Protection Assay (RPA) Steve Scherer
TA cloning nightmare Steve Scherer
suitable loading controls for RPA's in diabetic vs. normal rats Steve Scherer
Excision of LambdaZap Products Robert A. Schoenfeld
HELP!---Immunoaffinity column doesn't work Jan Schroeder
p53's apoptotic function Bob Scibienski
cracked gels? Bart Sefton
CNBr and phosphate groups Bart Sefton
Sfi 1 enzyme Jeff 'Newt' Sekelsky
VP16 antibodies Palle Serup
SEAP ole morten Seternes
fidelity of reverse transcriptase Regina Shaw
Linear acrylamide (was --Re: glycogen as a carrier) Glen Shearer
Northern blots without formaldehyde Glen Shearer
how to get rid of ethidium bromide??? Brian M. Shewchuk
RNase protections assays. Too much salt? Brian M. Shewchuk
Polymerase fidelity and KlenTaq suppliers Brian Shipley
? Re: Representational Difference Analysis Teresa Shippy
Genetically Directed RDA? Teresa Shippy
Primer design (Oligo) Benny Shomer
Wizard PCR purification from agarose gel Song-Kun Shyue
Can dessicants be dried in microwave? Nathan Siemers
G418 Madhu Singh
inducible eukaryotic expression Robert Slany
Transfection of HL60 Robert Slany
isolation of small DNA fragments Jeff Smerage
PCR through GC-rich Koen De Smet
Gibco WWW site Chuck Sohaskey
RNA extraction from S. cerevisiae Tan Nguan Soon
Background in Southern Blots Keld Sorensen
ELISA reagent supplier help needed! Keld Sorensen
fluorescently labelled protein Keld Sorensen
Mercaptoethanol Shadows Keld Sorensen
Glycoprotein Detection Keld Sorensen
Storage of serum in frost-free freezers? Keld Sorensen
direct blotting system Keld Sorensen
FBS users PLEASE READ - IMPORTANT Keld Sorensen
Free Sulphydryl detection Keld Sorensen
Best chemiluminescence for W.blotting Keld Sorensen
Best chemiluminescence for W.blotting Keld Sorensen
Tyramide Signal Amplification Keld Sorensen
concentration of biotinylated proteins Keld Sorensen
Seeking "Bioconsumer Review" Keld Sorensen
A fastidious contaminant Giorgio Spagnol
juks Mart Speek
Amersham Hybond N problem Cathy Sprankle
PCR through GC-rich Holger Steinlechner
Taq Extender--degrading A overhangs! Holger Steinlechner
20% gel drying Kaj Stenberg
Detection of molecular weight marker bands on +tively charged membranes Ed Stephenson
German drug screening, assay, metabolism vendors? Philip W. Stephenson
used equipment Jon Stewart
Excision of LambdaZap Products Jon Stewart
RNA Oligo supplier??? John Storring
Help needed with GST fusion protein. (pGEX vector) CBE Graduate Student
PCR-SSCP ON ABI 373 WITH GENESCAN 672 Carol Stugard
Internalization of the protein into cultured cells Yoo Hun Suh
TA cloning nightmare Martin Suhr
actinomycin D William Sun
anti 5-lipoxygenase antibodies Sailesh Surapureddi
Mercaptoethanol Shadows Frank Taddeo
RNA mass ladder greater than 10kb Steven Tait
IFN-treated cell cDNA library Seng-Lai Tan
Hsp70 Expression Vectors Seng-Lai Tan
IMMUNOAFFINITY COLUMN DOESN´T WORK Tanja
Opinion poll: Clonetech Matchmaker libraries Jil Tardiff
Looking for a histochemical ATPase protocol... Jil Tardiff
***Multiplex PCR Hell****** Dr G R Taylor
Sequencing Gel Rigs for SSCP Dr G R Taylor
PCR HELP! DXS52, Xq28 (St14 VNTR) anybody with experience? Dr G R Taylor
PCR can not get intense bands Dr G R Taylor
PCR can not get intense bands Dr G R Taylor
What is "Cleavase" Dr G R Taylor
GIBCO:Gene-trapper Kit Tegelk at ear002.uni-muenster.de
Taq Extender--degrading A overhangs! Jeffrey Shane Temple
Immuno(histo)fluorescence: what's the best method? Tommy the Terrorist
Can dessicants be dried in microwave? Tommy the Terrorist
rare cutters 10-12 bp exist? Nancy Terryn
Do you know? Tess
In vivo expression vectors Job Tharappel
source for ganciclovir Lyn A. Thet
electroporation of Streptomyces sp. Kelly J. Thibault
Anyone heard of pCMV7? (fwd) Jan Ulrik Thorup
Agarose and preparative gels... Anthony Tomlinson
Drying sequencing gels Anthony Tomlinson
how to get rid of ethidium bromide??? Anthony Tomlinson
SOS Call to all experinced "Sequencers" Anthony Tomlinson
Amersham Hybond N problem Tori
Primer extention analysis by PCR Toshio
Primer extension by rTth Reverse transcriptase ? Toshio
Help primer extention analysis Toshio
Assay of vitamins Krzysztof Treder
Acetylcholinesterase (AChE) regeneration after DFP? Achim Treumann
p53's apoptotic function Jean-Philip Truman
Reuse of Qiagen Ni-NTA Spin columns?? Bradley Turner
Source of Proteoglycans? Bradley Turner
SolarisX86 Bradley Turner
A260 or Fluoromete ??? Which is correct Bradley Turner
Inoue competent cell procedure Bradley Turner
Inoue competent cell procedure Bradley Turner
Seeking Proteoglycan Source Bradley Turner
Hsp70 Expression Vectors Bradley Turner
Seeking "Bioconsumer Review" Bradley Turner
Mycoplasma detection kits, and mycoplasma effects Bradley Turner
Rhodobacter disruption Craig Turner
Protein gel Michael J. Tuvim
A260 or Fluoromete ??? Which is correct Shaun Tyler
PCR contamination Shaun Tyler
Plant Expression Vectors Masashi Ugaki
Lignin Enzymes wanted Paul E. Ulanch
Thymidine kinase antibody Hemayet Ullah
NEEDED: codon bias table for Pseudomonas Hemayet Ullah
Thymidine Kinase Hemayet Ullah
SEAP Unregistered User
receptor cloning in mammalial cells Uwe
optimize mammalial cloning Uwe
Prok. expression vectors; anything that is not based on Lac operon and Amp R? Edgar Valencia
SOS!--PCR restriction cut Edgar Valencia
info about NTCB Patrick Van
Store DNA concentrated or dilute? Margon Vandongen
Store DNA concentrated or dilute? Margon Vandongen
TA cloning nightmare Margon Vandongen
Leaky Mini-ProteanII unit. Margon Vandongen
PCR mutagenesis and proofreading polymerase Margon Vandongen
Site directed mutagenesis kits -recommend? Margon Vandongen
Taq Extender--degrading A overhangs! Margon Vandongen
Expand PCR and TA cloning kits? Margon Vandongen
TNT kit with T7 RNA Pol Margon Vandongen
5'-> 3' Yegor Vassetzky
Getting cells off plates Tom Vink
PMSF timing Marc Visconti
Robot Cycler Marc Visconti
info on HPLC and related techniques Marc Visconti
Anyone heard of this company? Marc Visconti
Anyone successfully doing RNAse protection Assays? Jim Voeller
Unltracompentent cells Lee Vogel
Opinion poll: Clonetech Matchmaker libraries Lee Vogel
Cutting Qiagen miniprebs?? Bjoern Voldborg
Req: Electroporation of myloid and megakaryocyte cell lines, HELP?? Carmelo Volpe
Help needed for DNase I Footprinting Dominic Voon
Simple sequencing gel readerS WSchick at aol.com
Alamar inquiry WSchick at aol.com
(none) WSchick at aol.com
Sequencing Gel Rigs for SSCP WSchick at aol.com
SDS PAGE buffers cost saving WSchick at aol.com
2D-Software WSchick at aol.com
What is Touchdown PCR? NOT IDAHO WSchick at aol.com
Green Fluorescent Protein WSchick at aol.com
Pumps for Zonal Rotors WSchick at aol.com
A260 or Fluoromete ??? Which is correct WSchick at aol.com
A260 or Fluoromete ??? References WSchick at aol.com
Reply: DN Ligation UV Filter WSchick at aol.com
PCR in Nepal WSchick at aol.com
Seeking "Bioconsumer Review" FREE WSchick at aol.com
PFGE references? WSchick at aol.com
nicking DNA plasmids on purpose Mark Walberg
Getting cells off plates Edward Wang
NEEDED: codon bias table for Pseudomonas Peter Wang
Prok. expression vectors; anything that is not based on Lac operon and Amp R? Peter Wang
Getting cells off plates Pearse Ward
Luminimetry with luciferase: wavelength please! Glenn A. Warr
Isolation of nulcei Kristina Warton
RXR antibodies mike Watson
Wizard PCR purification from agarose gel A. John Watson
***ANYONE KNOW OF A GOOD FREEBIE PLASMID DRAWING PROGRAM?**** Dr Mark Watson
**Anyone have a murine Interferon gamma expressing plasmid or cassette??*** Dr Mark Watson
PCR-primer with T7 Promotor for TK Friedemann Weber
Quick Protein Concentration Assays Lee Weber
Fog in In Vitro Translations Lee Weber
Leaky Mini-ProteanII unit. John J Weiland
FIAU-Where can I buy it? Georg Weitzer
Gel documentation system K. Wernars
silver stain Michael West
Rainin edp plus Lesley Weston
ALF-express Toni Whistler
how to incubate Ab/membrane? J Quyen Wickham
Strep fusion protein vector? William_P_Prichett
CNBr and phosphate groups Randall Willis
Glycoprotein Detection Iain Wilson
Robot Cycler Troels Wind
Sequence-primer for lambda Pl? Troels Wind
Exogenous ATP supply - help requested Neil Winegarden
Background in Southern Blots Harry J. Witchel
Can dessicants be dried in microwave? Harry J. Witchel
Purifying clean plasmid vectors for ligation Harry J. Witchel
Purifying clean plasmid vectors for ligation Harry J. Witchel
Purifying clean plasmid vectors for ligation Harry J. Witchel
Protocol for Re-using of Qiagen tips Harry J. Witchel
PCR contamination-HELP! Harry J. Witchel
isolation of small DNA fragments Harry J. Witchel
Protocol for Re-using of Qiagen tips Harry J. Witchel
Genetic Meeting Jena Johannes Woestemeyer
Fog in In Vitro Translations Arthur Wohlwill
PCR HELP! DXS52, Xq28 (St14 VNTR) anybody with experience? John Wolff
Can dessicants be dried in microwave? Donald Chun Kit Wong
Flow Cytometer Surasakdi Wongratanacheewin
Antibody(ies) to Rat Aldolase(s)..........anybody? David Woodcock
Primer extention analysis by PCR Russell L Wrobel
FPLC from Perseptive Biosystems? XZHENG at GAMMA.IS.TCU.EDU
Any mass spec for protein sequencing under $30,000? XZHENG at GAMMA.IS.TCU.EDU
5' RACE Mayumi Yagi
RNA isolation Mayumi Yagi
Modified pGEM? Angel Yanagihara
Colourimetric CAT assay? Oksana K. Yarosh
Hirt extracts Ian A. York
anti-HA cross reaction? Ian A. York
Mycoplasma detection kits, and mycoplasma effects Ian A. York
SOS!--PCR restriction cut Wenjin Yu
Primer Design Xiu Yu
Lipase assay ZOOMmmm
Want: Simple sequencing gel reader Frances Hannan Zoology
Carb vs Amp - whats the story? Frances Hannan Zoology
plasmid cDNA library amplification Frances Hannan Zoology
Protein inhibitor(s) during immunoprecipitation ? Dr. P.A. de Zoysa
cDNA library kits-your choice Don Zuhn
Distinct bands in restriction dig. of genomic DNA ae at dna.bio.warwick.ac.uk
inosine primers Tm?? eric anderson
How does pH effect Terrific Broth? asdas
Any mass spec for protein sequencing under $30,000? asdas
any suggestion for electron autoradiography system b726711 at mailserv.cuhk.hk
Best chemiluminescence for W.blotting bbraun at ucla.edu
Northern hybridizations bbraun at ucla.edu
commercial adenovirus vectors bezstarosti at bc1.fgg.eur.nl
CIBA-GEIGY phone#? bio_hannan at emuvax.emich.edu
SYBR Green staining of PAGE/urea gels bmay
New life for old PCR templates? bmay
Alamar inquiry bolivs at ene.engg.upd.edu.ph
Gibco's WWW site? john brennand
Gibco's WWW site? john brennand
Help: Lambda purification kits john brennand
Site directed mutagenesis kits -recommend? john brennand
A260 or Fluoromete ??? Which is correct john brennand
Exogenous ATP supply - help requested john brennand
messy agarose bands john brennand
A260 or Fluoromete ??? Which is correct john brennand
Gibco's WWW site? john brennand
Inoue competent cell procedure john brennand
Gibco's WWW site? john brennand
DMSO and bacteriophage john brennand
Scintillation Counting Without Scintillation Fluid john brennand
Phage DNA Isolation john brennand
Pellet paint ? john brennand
EcoRI adaptors john brennand
Linear acrylamide (was --Re: glycogen as a carrier) john brennand
Transfection of proteins into cells: HOW? brett
SDS-PAGE protein probs paul mc bride
Biotinylated Primer for S1 probe? phillip buckhaults
Chemiluminescence, western blotting, problems... phillip buckhaults
Primer extension by rTth Reverse transcriptase ? phillip buckhaults
Automated sequencing reactions phillip buckhaults
Help-no plasmid!!! burklab at aecom.yu.edu
good sequencing unit christine-elsik at ansc.tamu.edu
fluorescently labelled protein davesmith at bioch.tamu.edu
Rainin edp plus batteries davesmith at bioch.tamu.edu
longggg mRNA transcript benan dincturk
Spinach library benan dincturk
long mRNAs benan dincturk
inducible eukaryotic expression ege
2D-Software eqpcmv at univ-lille1.fr
2D-Software eqpcmv at univ-lille1.fr
Representationel difference analysis ? bjorn eriksson
Fragment prep. using QiaTip? bjorn eriksson
Cutting PCR-created restriction sites futers at biovax.leeds.ac.uk
POST DOC AVAILABLE gabre
Zonal Rotor vibrations craig gatto
HELP! GST column with Tris-Saline? sujoy ghosh
Help: Rapid determination of copy No. of a gene in bacterial chromosome. gwade at physiology.uwo.ca
Problems with Qiagen Maxipreps? helix.nih.gov
Wanted: tk promoter - b gal construct helix.nih.gov
Cloned DNA will not be cut hilde.nilsen at unigen.unit.no
(none) Administrador de iihema.fmed.uba.ar
(none) Administrador de iihema.fmed.uba.ar
(none) Administrador de iihema.fmed.uba.ar
ANNOUNCEMENT ingen at ingenlabs.com
Siliconized coverslips for In Situ Hybridization ingen at ingenlabs.com
Primer Design j.r.stephen
ELISA reagent supplier help needed! jchristn at umich.edu
Help! Contract protein sequencing and phage display jchristn at umich.edu
recombinant thrombin jmorrey
? differntial PCR jms5 at Lehigh.EDU
RNA isolation jms93
DNA sequence for rat jms93
Primer Design jms93
Protocol for RT-PCR in shrimp jms93
Methylen blue DNA staining joaroyo at samba.cnb.uam.es
Need to remove nucleic acids from nuclear extracts joestudent at mit.edu
DNA precipitation? steve johnson
List of Genome projects david johnston
Daudi-Permanent cell line kim myung jong
western problem kim myung jong
Help : Hygromycin dose for Daudi kim myung jong
NdeI: cutting problems?? anyone else?? seems silly! jovermeyer at shrsys.hslc.org
Inverse PCR jovermeyer at shrsys.hslc.org
Tungsten particles for biolistic techniques? klas at kvl.dk
Membrane properties kramerse at wmvx.lvs.dupont.com
Detection of molecular weight marker bands on +tively charged membranes labimap
DNA sequence for rat lcwilliams at medusa.unm.edu
Request for RELIABLE ssDNA rescue protocol for sequencin liz_caetta at stratagene.com
Nicotiana spp. logan
NdeI: cutting problems?? anyone else?? seems silly! (fwd) logan
Mercaptoethanol Shadows logan
SDS PAGE buffers cost saving logan
Northern hybridizations logan
PCR stutters through oligo-A regions logan
req. opinions of Ericomp thermocycler lonnie
magnetophoretic systems lonnie
Bmh71-18 strain lperal
small PCR product-->ligation luis
Leaky Mini-ProteanII unit. mdpjr at cc.newcastle.edu.au
Leaky Mini-ProteanII unit. mdpjr at cc.newcastle.edu.au
SDS PAGE buffers cost saving mdpjr at cc.newcastle.edu.au
cDNA-Library of monocytes melkony at wwupop.uni-muenster.de
Automated sequencing reactions mikel at vaxc.cc.monash.edu.au
Help about blood storage please! mikem at s-and-s.com
are introns neccessary for transgene expression milstone
Anyone have pcDNA II? mjb1 at Lehigh.EDU
DNA precipitation? daniel morris
NdeI: cutting problems?? anyone else?? seems silly! (fwd) daniel morris
***Multiplex PCR Hell****** negar moshiri
pcDV1 sequence mpoulin at s-and-s.com
methylation of DNA in mice mrtour at stud.med.cornell.edu
p53's apoptotic function mrtour at stud.med.cornell.edu
POSITION AVAILABLE: Research Asst. Grtful ned
HIV p24 Antigen Administrador del nodo
e-mail Piatak Administrador del nodo
storage of E.coli Administrador del nodo
Wizard maxipreps o0c9869 at tam2000.tamu.edu
single band from rRNA from brachionus sp. paco
Pea genomic library paco
Aedes graciela panzetta
DH5 alpha chip peele
TRI-REAGENT chip peele
TRI-REAGENT chip peele
Isolation of RNA chip peele
Abbreviated miniprep protocol... Incredible! pgegen at rnaworld.bio.ukans.edu
Info about Auto Sequencer...! pramod at nii.ernet.in
PCR mutagenesis and proofreading polymerase prscholl
RNA isolation- the long and the short of it... przemko
Looking for supplier pubh021
Circular Dichroism r.jackson at ic.ac.uk
cracked gels? albano renee
Yolky DNA s060965 at AIX1.UOTTAWA.CA
Isolation of nulcei s1050589 at iplabs.ins.gu.edu.au
Isolation of nulcei s1050589 at iplabs.ins.gu.edu.au
Hsp70 Expression Vectors samueh01 at mcrcr6.med.nyu.edu
Peptide SLIGRL schille at uni-muenster.de
test scnomura at gan2.ncc.go.jp
UDP preparation shokhen
Protein/carbohydrate chemist wanted simon
Pink fluorescein?? simon
Heat lability of DEPC simon
Getting cells off plates simon
What is Touchdown PCR? spyong at pc.jaring.my
Heterologous probe a problem in Southern spyong at pc.jaring.my
SOS Call to all experinced "Sequencers" spyong at pc.jaring.my
PCR through GC-rich sreesh
Re-post: PCR through GC-rich sreesh
USB t4 DNA ligase ----PROBLEMS? teb3 at Lehigh.EDU
Sfi 1 enzyme tera
Sfi1 enzyme tera
Sfi1 enzyme tera
Best chemiluminescence for W.blotting tera
phage display using Stratagene's Surfzap Kit tera
phage display using Stratagene's Surfzap Kit tera
Site directed mutagenesis kits -recommend? tera
Expand PCR and TA cloning kits? tera
Tyramide Signal Amplification mark throsby
Anybody working with VEGF? tick
Lignin Enzymes wanted tigney at compusmart.ab.ca
hydrogenation Equipment Needed tigney at compusmart.ab.ca
Terminal transferase buffer tjames
PCR mutagenesis and proofreading polymerase upyers
Extra Long PCR reamplification upyers
Help with Transcription Factors Database? username at bay.cc.kcl.ac.uk
cell line vdilbeck at dbm.ulb.ac.be
Amersham Hybond N problem vilimf01 at mcrcr6.med.nyu.edu
Anyone successfully doing RNAse protection Assays? vilimf01 at mcrcr6.med.nyu.edu
Magnetic RNA isolation: do-it-yourself beads vilimf01 at mcrcr6.med.nyu.edu
Inverse PCR vilimf01 at mcrcr6.med.nyu.edu
RT of G-cap into C? vilimf01 at mcrcr6.med.nyu.edu
Yolky DNA vilimf01 at mcrcr6.med.nyu.edu
5' RACE vilimf01 at mcrcr6.med.nyu.edu
PCR contamination-HELP! vilimf01 at mcrcr6.med.nyu.edu
PCR stutters through oligo-A regions vilimf01 at mcrcr6.med.nyu.edu
Taq Extender--degrading A overhangs! vilimf01 at mcrcr6.med.nyu.edu
PC version of FASTA vipond at BSA.BRIS.AC.UK
Northern blot and NybondN+membrane ruth e.w. walker
removing RNA ruth e.w. walker
Wizard preps ruth e.w. walker
Very pure total RNA free of DNA? ruth e.w. walker
Wizard PCR purification from agarose gel ruth e.w. walker
Help! My RNA has solidified!! wapling at staff.monash.edu.au
Siliconized coverslips for In Situ Hybridization wgarrett at GGPL.ARSUSDA.GOV
GFP fusion problems fred wouters
anti 5-lipoxygenase antibodies fred wouters
Mercaptoethanol Shadows xzheng at GAMMA.IS.TCU.EDU
electroporation yxu
Alternative to autoclaving for denucleasing plasticware zeus

Last message date: Sun Mar 31 23:35:03 EST 1996
Archived on: Sun Mar 6 20:59:58 EST 2005

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