Methods March 1996 Archives by date

Messages sorted by: [ thread ] [ subject ] [ author ] Index

Starting: Fri Mar 1 01:28:18 EST 1996
Ending: Sun Mar 31 23:35:03 EST 1996
Messages: 1286

Alkaline gels-Better method? Amie Franklin
nicking DNA plasmids on purpose Amie Franklin
Density gradient cell separation and streptavidin-agarose? G.P.
FIAU-Where can I buy it? Georg Weitzer
Non-detergent containing lysis buffer for primary cell culture? G.P.
G418 Mike
HELP! GST column with Tris-Saline? Cornelius Krasel
nicking DNA plasmids on purpose Zophonias O. Jonsson
LexA antibodies? Ian Graham
Deuterium oxide in density gradient? Dr E. Buxbaum
HELP! looking for N-ras intronic sequence. Erwin Pauws & Carrie Ris
freeze-squeeze Dr. K.Hoare
Staining DNA in Agarose with Methylene Blue Joseph C. Bagshaw
Alkaline Phosphatase substrates Gabriele Kerber
pHOOK (CaptureTec system) Graham Atherton
DNA sequence for rat lcwilliams at medusa.unm.edu
Amersham Hybond N problem BRITTA SCHULZ
pHOOK (CaptureTec system) Nigel Kenward
mouse Raf genomic clones and sequence wanted Gregory Buzard
Abbreviated miniprep protocol... Incredible! Alexander Kraev
HELP! looking for N-ras intronic sequence. Gregory Buzard
Aedes graciela panzetta
Amersham Hybond N problem Alexander Kraev
***Multiplex PCR Hell****** negar moshiri
5' RACE Peta C. Bonham-Smith
PCR probes by incorporation of 32P ? Geoffrey Neale
Anyone successfully doing RNAse protection Assays? Helmut
G418 Madhu Singh
Magnetic RNA isolation M.W. SMITH
inducible eukaryotic expression Robert Slany
Request for RELIABLE ssDNA rescue protocol for sequencin liz_caetta at stratagene.com
Adenovirus expression systems Valeri Krougliak
Alkaline Phosphatase substrates martin LEACH
Can one gene be used to select for OR against.... Paul N Hengen
5' RACE Mayumi Yagi
freeze-squeeze Paul N Hengen
Microplate technology Dr._Jose_Romero_at_WRAIR3__WASHDC at WRSMTP-CCMAIL.ARMY.MIL
Amersham Hybond N problem Paul N Hengen
Ribonuclease Protection Assay (RPA) Osama
Sfi 1 enzyme tera
nicking DNA plasmids on purpose Mark Walberg
Problems with Qiagen Maxipreps? helix.nih.gov
Alkaline Phosphatase substrates Pierce Chemical Co
used equipment Michael Bausher
Sequencing after G run Michael Gorry
ELISA reagent supplier help needed! jchristn at umich.edu
Help! Contract protein sequencing and phage display jchristn at umich.edu
Does anyone have an antibody to HSVtk? Phil Potter
purification of 140 Kbp from agarose Riekeltje Koedood
freezer purchase Riekeltje Koedood
rat cardiomyocyte cell line, who has one? Daniel
Making His-tag affinity resin Rita Lim
resending CBN info "Thro, Ann Marie ", 3701, by way of Prakash at acd.tusk.edu C. S. Prakash
alpha-S dNTPs Michael Lichten
inhibition of NF-kappa B at rzmail.uni-erlangen.de
DH5 alpha chip peele
Abbreviated miniprep protocol... Incredible! pgegen at rnaworld.bio.ukans.edu
E. coli sec mutants Elliott Dawson
Ps fluorescens biovars? Don Chen
Background in Southern Blots Jerry Litt
BioRad GeneLite Kit Amie Franklin
Mitochondrial DNA extraction? Anita Gould
DH5 alpha David S. Lee
In situ hybridization Gabriel Cheung
SfiI enzyme Andrei Popov
test scnomura at gan2.ncc.go.jp
nicking DNA plasmids on purpose Ned Mantei
purification of 140 kbp from agarose Andrei Popov
Dye and terminator chemistry Microplate at eworld.com
Sfi 1 enzyme Jeff 'Newt' Sekelsky
Lignin Enzymes wanted tigney at compusmart.ab.ca
hydrogenation Equipment Needed tigney at compusmart.ab.ca
recombinant thrombin jmorrey
Amersham Hybond N problem vilimf01 at mcrcr6.med.nyu.edu
SYBR Green staining of PAGE/urea gels bmay
Northern controls John Morlan
New life for old PCR templates? bmay
Anyone successfully doing RNAse protection Assays? vilimf01 at mcrcr6.med.nyu.edu
Biological Data Transport info R. Scott Jokerst
Anyone have indepth info on annealing temps and unzipping temp or equation for PCR components? JCM
***Multiplex PCR Hell****** Dr G R Taylor
Magnetic RNA isolation Reese Bolinger
Biotinylated Primer for S1 probe? phillip buckhaults
RNA isolation DANIEL Y KIM
purification of a 140 kb fragment Andrei Popov
EtOH + PBS = precipitation? Jim Hutchins
Superbroth for EVERYONE! (was NOT, but now IS) Robert Randolph
C127I cells & 6-TG Ashraf R. Dallol
Thymidine kinase antibody Ashraf R. Dallol
Anyone successfully doing RNAse protection Assays? Jim Voeller
5' RACE Steve Scherer
Ribonuclease Protection Assay (RPA) Steve Scherer
freeze-squeeze Michael Gillings
mitochondrial DNA database Jim Cummins
PCR Primers Database - UPDATE. Jim Cummins
Superbroth for EVERYONE! (was NOT, but now IS) Alex Merz
Daudi-Permanent cell line kim myung jong
freeze-squeeze Nigel Kenward
RNA isolation jms93
DNA sequence for rat jms93
Plasmid Drawing Programme Tom Dougherty
Microplate technology Microplate at eworld.com
Aminopropanol mutant Jean-Francois Mayaux
Microinjection: where to find info about? Stefan Kahlert
Transfection of proteins into cells: HOW? Stefan Kahlert
Polymerases, anyone!?!?!? Brett Beitzel
Peptides Darren Boehning
Unltracompentent cells Lee Vogel
Plasmid Drawing Programs - where to find... R. Scott Jokerst
Cutting PCR-created restriction sites futers at biovax.leeds.ac.uk
Sequence of Lambda DASH II polylinker Anton Scott Goustin
Alpha Satellite Probes for Mice??? Where can you get them? FOR FISH Graham Dellaire
Protein gel Michael J. Tuvim
Background in Southern Blots Keld Sorensen
ELISA reagent supplier help needed! Keld Sorensen
fluorescently labelled protein Keld Sorensen
fluorescently labelled protein davesmith at bioch.tamu.edu
Anyone have pcDNA II? mjb1 at Lehigh.EDU
Plasmid Drawing Programme Soren K. Rasmussen
Unspecific hybridization to plasmid-vector Manuel G. CLAROS
COS-cells David L. Haviland, Ph.D.
PCR probes by incorporation of 32P ? Robert Horton
Sequencing after G run Paul N Hengen
large-scale prep of ssDNA Yuandan Lee
Transcription factors and transfections Karen Kroeger
Transfection of proteins into cells: HOW? brett
electroporation of Streptomyces sp. Kelly J. Thibault
Can dessicants be dried in microwave? Donald Chun Kit Wong
Transfection of proteins into cells: HOW? Brian T. Greuel
Can dessicants be dried in microwave? Michelle.Gleeson at SMTPGWY.AGRIC.NSW.GOV.AU
Can dessicants be dried in microwave? Michelle.Gleeson at SMTPGWY.AGRIC.NSW.GOV.AU
Amersham Hybond N problem Cathy Sprankle
Chemiluminescence, western blotting, problems... phillip buckhaults
anti-myristoylated glycine Ab Atsushi Ishikawa
Anyone have indepth info on annealing temps and unzipping temp or equation for PCR components? Eitan Rubin
freeze-squeeze Eitan Rubin
Anyone have indepth info on annealing temps and unzipping temp or equation for PCR components? Eitan Rubin
Can dessicants be dried in microwave? David Joyce
Why use 3´to5´exo-negative DNA-Polymerases? Horst Ibelgaufts
COS-cells Mike
Background in Southern Blots Harry J. Witchel
inducible eukaryotic expression Mike
Can dessicants be dried in microwave? Harry J. Witchel
rat cardiomyocyte cell line Frank Schaap
Native PAGE @ pH < 6.5 Christopher Walentas Bioc
Superbroth for EVERYONE! (NOT) elizabeth Ferguson
Transfection of proteins into cells: HOW? elizabeth Ferguson
Hydropathy plot program Wayne Coco
Sources of baculovirus expression systems Tracy Aquilla
Differential centrifugation of cells and streptavidin-agarose? G.P.
PMSF timing Marc Visconti
electroporation of Streptomyces sp. Dima Klenchin
(no subject) T. James
GAPDH mRNA half-life Peter G Bradford
TRI-REAGENT chip peele
Peptide SLIGRL schille at uni-muenster.de
used equipment Jon Stewart
Membrane properties Michail P. Sahsamanoglou
Chemiluminescence, western blotting, problems... Giampaolo Minetti
Northern blot and NybondN+membrane Joël Baguet
inducible eukaryotic expression Michail Belyavskyi
Polymerases, anyone!?!?!? Martin_K_Burnham at SBPHRD.COM
Can one gene be used to select for OR against.... Sanjay Krishnaswamy
PCR-SSCP ON ABI 373 WITH GENESCAN 672 Carol Stugard
Can dessicants be dried in microwave? Bernard Murray
Amersham Hybond N problem Tori
Biotech NewsReport C. S. Prakash
Want: Simple sequencing gel reader Bob Litt
TRI-REAGENT chip peele
RNA isolation Susan Patrick
Quick Protein Concentration Assays Lee Weber
Chemiluminescence, western blotting, problems... Bernard Murray
Chemiluminescence, western blotting, problems... John K. Troyer, PhD.
Touchdown PCR protocoll? Olivier Gandrillon
Non-detergent containing lysis buffer for primary cell culture? John K. Troyer, PhD.
Inoue Competent Cell procedure Richard Friedman
fluorescently labelled protein Computer
TA cloning nightmare Amna Saeed
juks Mart Speek
TA cloning nightmare Amie Franklin
Can dessicants be dried in microwave? Mark Dowton
Superbroth for EVERYONE! (NOT) Dima Klenchin
Help-no plasmid!!! Mark Griffiths
Terminal transferase buffer tjames
Want: Simple sequencing gel reader Phil Davey
HELP!---Immunoaffinity column doesn't work Jan Schroeder
Want: Simple sequencing gel reader Frances Hannan Zoology
GFP fusion problems fred wouters
Inoue protocol with reducing agents. And speedprep David Martin
Qiaex problems Lynne M Donegan
Primer Design j.r.stephen
Re- Touchdown PCR Protocol Brett Beitzel
fluorescently labelled protein Richard R. Hardy
Reagent Availabilty Michael Kane
large-scale prep of ssDNA Duke Groebe
activated T-cell library for 2-hyb?? Heidi Moss
Membrane properties kramerse at wmvx.lvs.dupont.com
rare cutters 10-12 bp exist? Nancy Terryn
large-scale prep of ssDNA Michael Benedik
Anyone successfully doing RNAse protection Assays? Tracy Aquilla
Homemade shaking incubator Bob Horton
Chemiluminescence, western blotting, problems... Hedrick Lab
rare cutters 10-12 bp exist? Gary K.
Representationel difference analysis ? bjorn eriksson
Prok. expression vectors; anything that is not based on Lac operon and Amp R? Hedrick Lab
Northern controls John Morlan
RNA isolation Mayumi Yagi
Northern blot and NybondN+membrane Gerald Downes
(no subject) Rick Carroll
cDNA library kits-your choice Don Zuhn
Hirt extracts Ian A. York
single-strand DNA break detection Scott Selby Med-Hem
COS-cells Allison Haggarty
RNA removal/quartz plate Rick Carroll
anti-HA cross reaction? Allison Haggarty
IMMUNOAFFINITY COLUMN DOESN´T WORK Tanja
Nicotiana spp. logan
ANNOUNCEMENT ingen at ingenlabs.com
PCR-SSCP ON ABI 373 WITH GENESCAN 672 Gregory Buzard
NEEDED: codon bias table for Pseudomonas Robert Johnston
ISO Plasmid Not1 & Mlu1 Sites DNAGuy
COS-cells Riekeltje Koedood
Help-no plasmid!!! burklab at aecom.yu.edu
anti-HA cross reaction? Ian A. York
Primer Extension Sequencing Steven Bressler
PCR Protocol for Mycoplasma Detection??? Carolyn Calnan
Prok. expression vectors; anything that is not based on Lac operon and Amp R? Edgar Valencia
Amersham Hybond N problems Michelle.Gleeson at SMTPGWY.AGRIC.NSW.GOV.AU
Prok. expression vectors; anything that is not based on Lac operon and Amp R? Mikhail Alexeyev
Robot Cycler Pascal_Bochet
resolution on SDS-PAGE From Jaume Canaves
Is B-Gal phosphorylated in mammalian cells? Steven Enkemann
Anti-microbials Paul
Heat lability of DEPC Adrian.Philbey at smtpgwy.agric.nsw.gov.au
Lignin Enzymes wanted Amie Franklin
Amersham Hybond N problem C.L.L.
Simple sequencing gel readerS WSchick at aol.com
Very pure total RNA free of DNA? Agnes S. Chan
Announce WWW: Analytical Ultracentrifugation/XL-A Borries Demeler
Alamar inquiry bolivs at ene.engg.upd.edu.ph
Expression of GST fusion Gary T. Chou
Help-no plasmid!!! Tim Barnett
electroporation Marcel Hoffmann
low recovery with PromegaPolyATtract kit ? Toshiharu Ishizuka
Lignin Enzymes wanted Mick Partis
Opinion poll: Clonetech Matchmaker libraries Lee Vogel
Zonal gradients Juergen Brosius
rare cutters 10-12 bp exist? Dr. Duncan Clark
Heat lability of DEPC Paul
rodent DNA and/or brains Juergen Brosius
G418 Henry Hoppe
Differential centrifugation of cells and streptavidin-agarose? Mary Meza
Casein antibodies Gustavo Nolasco
TA cloning nightmare Catherine I. DiOrio
Meeting - H.pylori Ronan O'Kennedy
Qiaex problems Catherine I. DiOrio
low recovery with PromegaPolyATtract kit ? Catherine I. DiOrio
Taq stability? Susan Jane Hogarth
Lignin Enzymes wanted Michael Bausher
large-scale prep of ssDNA Kiley R. Prilliman
Cycle Sequencing Robmit
Chemiluminescence, western blotting, problems... Giampaolo Minetti
Northern controls John Morlan
ISO Plasmid Not1 & Mlu1 Sites Mikhail Alexeyev
Magnetic RNA isolation CPGYIELD
RT-PCR and cDNA synthesis from bacterial total RNA Oliver Politz
influenza hemagglutinin abs Mauro
Wizard maxipreps Surgical Residence
DNA Isolation Kit Surgical Residence
Chemiluminescence, western blotting, problems... Surgical Residence
TA cloning nightmare Steve Scherer
Isolation of RNA chip peele
Leaky Mini-ProteanII unit. John J Weiland
Want: Simple sequencing gel reader Andy Phillips
Leaky Mini-ProteanII unit. Dima Klenchin
Spectrophotometric estimation of RNA SRINIVAS MUMMIDI
ANNOUNCE: tacg - Restriction Enzyme analysis tool for unix Harry Mangalam
vectors poly (A) Jo=?iso-8859-1?Q?=EBl BAGUET
Unspecific hybridization to plasmid-vector Ian A. Boussy
inducible eukaryotic expression ege
Prok. expression vectors; anything that is not based on Lac operon and Amp R? Hedrick Lab
Leaky Mini-ProteanII unit. Paul N Hengen
Leaky Mini-ProteanII unit. HIRANYA ROYCHOWDHURY
Self competition in EMSA Menglin Luo
receptor cloning in mammalial cells Uwe
NEEDED: codon bias table for Pseudomonas Peter Wang
NH4Ac/EtOH ppt of DNA from PCR ? Royal Botanic Gardens
optimize mammalial cloning Uwe
Store DNA concentrated or dilute? Susan Jane Hogarth
Magnetic RNA isolation: do-it-yourself beads Anton Scott Goustin
Trace Acetylation Protocol From Jaume Canaves
Linking an oligonucleotide to an antibody Anton Scott Goustin
electroporation yxu
baculovirus co-infection BIOTEC54
Immuno(histo)fluorescence: what's the best method? Tommy the Terrorist
Can dessicants be dried in microwave? Tommy the Terrorist
Alamar inquiry WSchick at aol.com
Protein/carbohydrate chemist wanted simon
Very pure total RNA free of DNA? Amie Franklin
Can dessicants be dried in microwave? Mark Hill
any suggestion for electron autoradiography system b726711 at mailserv.cuhk.hk
Robot Cycler Troels Wind
Polyacrylamide gel fixation for DNA Danny Leung
A fastidious contaminant Giorgio Spagnol
TA cloning nightmare Dr. Duncan Clark
Prok. expression vectors; anything that is not based on Lac operon and Amp R? Dr. Duncan Clark
Taq stability? Dr. Duncan Clark
what is a cytofectin? Horst Ibelgaufts
NH4Ac/EtOH ppt of DNA from PCR ? Dr. Duncan Clark
Sequencing Gel Rigs for SSCP Dr G R Taylor
TA cloning nightmare Bob Horton
Taq stability? Bob Horton
NH4Ac/EtOH ppt of DNA from PCR ? DSeegert
TA cloning nightmare Martin Suhr
Looking for supplier pubh021
Clone fingerprinting Mr. A.R.J. Curtis
superbroth protocol finally finished Guivarc_h Dominic
Taq stability? Dubear Kroening
(none) WSchick at aol.com
Clone fingerprinting Mr. A.R.J. Curtis
Heat lability of DEPC P. Mertens
Transfection of proteins into cells: HOW? Stefan Kahlert
removal of starch (fwd) Baozhu Guo
Very pure total RNA free of DNA? Simon Dawson
Casein antibodies Simon Dawson
Prok. expression vectors; anything that is not based on Lac operon and Amp R? Mikhail Alexeyev
Tungsten particles for biolistic techniques? klas at kvl.dk
Opinion poll: Clonetech Matchmaker libraries Simon Dawson
CIBA-GEIGY phone#? bio_hannan at emuvax.emich.edu
RT-PCR Burkhard Hassel
Expression of GST fusion Bernard Heymann
Thymidine kinase antibody Hemayet Ullah
NEEDED: codon bias table for Pseudomonas Hemayet Ullah
Thymidine Kinase Hemayet Ullah
RT efficiency Michael HERMAN
(no subject) Bertrand COLLET
Store DNA concentrated or dilute? Duke Groebe
Store DNA concentrated or dilute? Duke Groebe
Protocol for Re-using of Qiagen tips Trevor Bezdek
Clone Fingerprinting --SSCP Gregory Buzard
Polyacrylamide gel fixation for DNA Gregory Buzard
ISO Plasmid with Not1 & Mlu1 Site DNAGuy
ISO Plasmid with Not1 & Mlu1 Site Gary K.
Nuclease P1, Penicillium citrinum ProZyme
need Serva contact info Gary K.
Store DNA concentrated or dilute? Margon Vandongen
Streptavidin in purification by affinity Dr._Jose_Romero_at_WRAIR3__WASHDC at WRSMTP-CCMAIL.ARMY.MIL
PCR through GC-rich MAN!!!
Polyacrylamide gel fixation for DNA DNAGuy
Transfection of proteins into cells: HOW? Dima Klenchin
need Serva contact info Dima Klenchin
PCR probes by incorporation of 32P ? Joseph C. Bagshaw
Cycle Sequencing Joseph C. Bagshaw
Northern blot and NybondN+membrane ruth e.w. walker
removing RNA ruth e.w. walker
Wizard preps ruth e.w. walker
REPOST Homemade Shaking Incubator Bob Horton
Transfection of proteins: HOW? Nikolai Chitaev
Very pure total RNA free of DNA? ruth e.w. walker
Robot Cycler Marc Visconti
<a href="http://www.bio.net/hypermail/sponsor.html"><img src="http://www.bio.net/hypermail/sponsor.gif"></a><br>bionet.molbio.methds-reagnts archives: HELP!---Immunoaffinity column doesn't work Nikolai Chitaev
Wizard PCR purification from agarose gel ruth e.w. walker
Help: Rapid determination of copy No. of a gene in bacterial chromosome. Xiang Li
(Q) Problem w/silver enhancement of gold particles Kenneth J McNeil
Best chemiluminescence for W.blotting MALINI at JASON.UTHCT.EDU
Magnetic RNA isolation: do-it-yourself beads vilimf01 at mcrcr6.med.nyu.edu
FPLC from Perseptive Biosystems? XZHENG at GAMMA.IS.TCU.EDU
Primer Design Ed Castro
Western blot mystery: update Pamela Norton
elisa plates??? LMordo
Clone fingerprinting Andy Phillips
Looking for supplier Andy Phillips
Allelic Ladder-source needed? Garry P. Larson
Catalytically active purified Ras? Andrew Lange
Light Sensitivity of Tris Ferland Louis H.
Sequencing Gel Rigs for SSCP WSchick at aol.com
NdeI: cutting problems?? anyone else?? seems silly! Heidi Moss
Opinion poll: Clonetech Matchmaker libraries Jil Tardiff
ATTO supplier JoanBoyce at aol.com
A fastidious contaminant John Ladasky
Store DNA concentrated or dilute? Richard Daines
Expression of GST fusion John Elliott
Wizard PCR purification from agarose gel Markku.M Jeltsch
Distinct bands in restriction dig. of genomic DNA Markku.M Jeltsch
Taq stability? Christoph Ahlers
Robot Cycler Kiley R. Prilliman
PCR through GC-rich Dr. Duncan Clark
NdeI: cutting problems?? anyone else?? seems silly! Dr. Duncan Clark
Gel Analysis - DNA ProScan David Ley
PCR method Andreas Ebrecht
RXR antibodies Eddie Diehl
Wizard maxipreps o0c9869 at tam2000.tamu.edu
RNAzolB...Still Available? Eric C. Anderson
How stable biotin-avidin? Marc Lamphier
Store DNA concentrated or dilute? Bernard Murray
NEEDED: codon bias table for Pseudomonas Shamima Akhter
Protocol for RT-PCR in shrimp Sittiruk Roytrakul - SCBC - 3536459
influenza hemagglutinin abs Thomas R. Anderson
Distinct bands in restriction dig. of genomic DNA Ned Mantei
Heat lability of DEPC Alexander Kraev
Cycle Sequencing Alexander Kraev
low recovery with PromegaPolyATtract kit ? Marc Lamphier
Help? Immunoprecipitation Marc Lamphier
Degenerate primers for PCR HelenCS
Distinct bands in restriction dig. of genomic DNA ae at dna.bio.warwick.ac.uk
Polyacrylamide gel fixation for DNA Gregory Buzard
HRPO linking to oligos? Chris Kafer
Boomerang DNA Amplification Web Page Kevin Ahern
PALA only from NCI? Andreas Schaffer
Polyacrylamide gel fixation for DNA Gregory Buzard
Leaky Mini-ProteanII unit. mdpjr at cc.newcastle.edu.au
Very pure total RNA free of DNA? Michael Gillings
The KlenTaq mystery Amna Saeed
Rhodobacter disruption Craig Turner
suitable loading controls for RPA's in diabetic vs. normal rats Nick Kallincos
How to set up ELISAs Yasuhito Abe
Transfection of proteins into cells: HOW? Nigel Kenward
How stable biotin-avidin? Zophonias O. Jonsson
Purifying clean plasmid vectors for ligation Harry J. Witchel
Leaky Mini-ProteanII unit. Giampaolo Minetti
RXR antibodies mike Watson
Hefe-Klonierung Georg Kroeger
Northern hybridizations PRETORIUS
ANNOUNCE: NAOMI Upgrade Simon M. Brocklehurst
SDS PAGE buffers cost saving WSchick at aol.com
NdeI: cutting problems?? anyone else?? seems silly! (fwd) Neil Harris
protoplast isolation JWU at LAN.TJHSST.EDU
Heat lability of DEPC Tracy Aquilla
Store DNA concentrated or dilute? Margon Vandongen
Salmon sperm DNA preparation Rachelle Leger
Protocol for Re-using of Qiagen tips Iain Fenton
The KlenTaq mystery Dr. Duncan Clark
Large scale low melt extraction Mike Holbrook
cell line vdilbeck at dbm.ulb.ac.be
Inverse PCR Helen J. Hathaway, Ph.D.
How stable biotin-avidin? Tehcnical Assistance / Pierce Chemical Co
ELISA reagent supplier help needed! Tehcnical Assistance / Pierce Chemical Co
SEAP Unregistered User
transformation problems GILLIAN NI BHARLO BOTANY PG
Salmon sperm DNA preparation Alexander Kraev
Carb vs Amp - whats the story? John Dixon
How stable biotin-avidin? Paul N Hengen
How stable biotin-avidin? Michael Giffin
thrombin source for fusion prot cleav. GLUXLAB
Magnetic RNA isolation: do-it-yourself beads Mark McCormick
Carb vs Amp - whats the story? Frances Hannan Zoology
SEAP ole morten Seternes
Purifying clean plasmid vectors for ligation Mikhail Alexeyev
actinomycin D William Sun
western problem kim myung jong
tips for blunt ended ligation! Kenneth Y. Ilio
1500+ scientists obtain free BCMD subscriptions - bcmd.msg [1/1] Ernest Beutler, M.D.
Help needed with GST fusion protein. (pGEX vector) CBE Graduate Student
fax of SCHOTT in Germany or in France Pierre Commercon
1500+ scientists obtain free BCMD subscriptions - bcmd.msg [1/1] Ernest Beutler, M.D.
SEAP Bernard Murray
Carb vs Amp - whats the story? Paul N Hengen
Large scale low melt extraction Paul N Hengen
tips for blunt ended ligation! Dilip Dias
tips for blunt ended ligation! Dilip Dias
Store DNA concentrated or dilute? Paul N Hengen
tips for blunt ended ligation! Mikhail Alexeyev
PtdIns(3,4,5)P3 Where do I get it? George W Chacko
Spectrophotometric estimation of RNA Anton Scott Goustin
GeneClean II NaI conc??? Walter Hill
Taq stability? Nicola Collie
How stable biotin-avidin? Dima Klenchin
SDS PAGE buffers cost saving Phillip Robinson
elisa plates??? Microplate at eworld.com
GeneClean II NaI conc??? Shelley Cole
Anyone successfully doing RNAse protection Assays? CAShug
Salmon sperm DNA preparation CAShug
transformation problems CAShug
Help! My RNA has solidified!! wapling at staff.monash.edu.au
Large scale low melt extraction Karl Fischer
REAP Bertrand COLLET
Tn5-GM Neil Crickmore Bioc
Internalization of the protein into cultured cells Seong-Hun Kim
Fragment prep. using QiaTip? bjorn eriksson
Large scale low melt extraction K.James
(none) Hajime Sawada
Large scale low melt extraction Alexander Kraev
35S-RFLPs Javier Quinteiro
Artifact in plasmid preps Dr. Duncan Clark
Northern hybridizations Stephen R. Lasky
Store DNA concentrated or dilute? Stephen R. Lasky
GeneClean II NaI conc??? Gary K.
TA cloning GILLIAN NI BHARLO BOTANY PG
Protocol for Re-using of Qiagen tips Mads Noerregaard-Madsen
VP16 antibodies Palle Serup
mRNA preps Rachelle Leger
Cloned DNA will not be cut hilde.nilsen at unigen.unit.no
test, please ignore Gary Kucera
Response to "Quick Protein Concentration Assays" BOYDSTON at MVAXGR.HUNTER.CUNY.EDU
human genomic library Markus Stefan Mueller
Testis library for two-Hybrid?? Michele Sallese
Distinct bands in restriction dig. of genomic DNA Eitan Rubin
TA cloning nightmare Margon Vandongen
cDNA library kits-your choice RMMMS at CUNYVM.CUNY.EDU
Purifying clean plasmid vectors for ligation Steven Goldberg
NdeI: cutting problems?? anyone else?? seems silly! (fwd) logan
Storage of serum in frost-free freezers? Eddie Diehl
tips for blunt ended ligation! Eddie Diehl
Sources of baculovirus expression systems RMMMS at CUNYVM.CUNY.EDU
Leaky Mini-ProteanII unit. Riekeltje Koedood
PCR through GC-rich Koen De Smet
How stable biotin-avidin? Mikhail Alexeyev
RNA extraction-whole blood Neil Burns
Salmon sperm DNA preparation Karl Fischer
Tn5-GM Mikhail Alexeyev
NdeI: cutting problems?? anyone else?? seems silly! (fwd) Gary Kucera
suitable loading controls for RPA's in diabetic vs. normal rats Steve Scherer
Mercaptoethanol Shadows Frank Taddeo
Glycoprotein Detection Neil Burns
Wizard PCR purification from agarose gel A. John Watson
req. opinions of Ericomp thermocycler Brad Fabbri
ANNOUNCE: Primers! for the Mac v1.0 Richard J. Resnick
Artifact in plasmid preps Mark R. Miglarese
Sfi1 enzyme tera
Sfi1 enzyme tera
Store DNA concentrated or dilute? Bernard Murray
Expression of GST fusion L. Fliegel
Mercaptoethanol Shadows Keld Sorensen
Mercaptoethanol Shadows logan
Glycoprotein Detection Keld Sorensen
Salmon sperm DNA preparation Riekeltje Koedood
Leaky Mini-ProteanII unit. George Rutherford
elimination of cross reaction in polyclonal antiserum David P Alt
Agarose and preparative gels... HIRANYA ROYCHOWDHURY
Ribozymes Bradley A. Arrick
SDS-PAGE protein probs paul mc bride
Opinions on vaccuum centrifuge Andrea Crampton
Leaky Mini-ProteanII unit. mdpjr at cc.newcastle.edu.au
SDS PAGE buffers cost saving mdpjr at cc.newcastle.edu.au
RNA extraction from S. cerevisiae Tan Nguan Soon
Genescan and Diff Display Mona
Purifying clean plasmid vectors for ligation Amie Franklin
Lignin Enzymes wanted Paul E. Ulanch
Mercaptoethanol Shadows xzheng at GAMMA.IS.TCU.EDU
POSITION AVAILABLE AT BIO-RAD LABORATORIES MacBoyee
Why serum starve before fos-jun induction ? Avirup Bose
Thymidine Kinase (Repost) Polash
Best chemiluminescence for W.blotting bbraun at ucla.edu
PC12 cells Philippe Lefebvre
Why serum starve before fos-jun induction ? Adrian.Philbey at SMTPGWY.AGRIC.NSW.GOV.AU
mRNA preps Adrian.Philbey at SMTPGWY.AGRIC.NSW.GOV.AU
***ANYONE KNOW OF A GOOD FREEBIE PLASMID DRAWING PROGRAM?**** Dr Mark Watson
Guinea Pig B-actin seq. Help! Mr N.C. Eastmond
Pink fluorescein?? simon
transformation problems P. Mertens
SDS PAGE buffers cost saving logan
Primer Design jms93
Protocol for RT-PCR in shrimp jms93
Peptide synthesis David Martin
Purifying clean plasmid vectors for ligation Harry J. Witchel
Purifying clean plasmid vectors for ligation Harry J. Witchel
Methylen blue DNA staining joaroyo at samba.cnb.uam.es
Protocol for Re-using of Qiagen tips Harry J. Witchel
Storage of serum in frost-free freezers? Keld Sorensen
Why serum starve before fos-jun induction ? Mark R. Miglarese
single band from rRNA from brachionus sp. paco
Gibco's WWW site? G.P.
Pea genomic library paco
inosine primers Tm?? Mr D.P. O'Hare
Wizard PCR purification from agarose gel Tae Gwon Oh
Primer design (Oligo) Bill Burnett
SpinBind Oliver S. DePeyer
SpinBind Oliver S. DePeyer
Can dessicants be dried in microwave? Nathan Siemers
Home-made reverse transcriptase. Richard Near
Re-post: PCR through GC-rich MAN!!!
Antisense gene knockout Richard Near
differential display Harm Deckers
Artifact in plasmid preps Paul N Hengen
Leaky Mini-ProteanII unit. Margon Vandongen
Spectrophotometer Cuvettes Mr N.C. Eastmond
Spectrophotometer Cuvettes Mr N.C. Eastmond
Storage of serum in frost-free freezers? Eddie Diehl
A question about in situ hybridization Martin Downes
MICROSEQUENCING N-BLOCKED PROTEINS Andy Brauer
HELP : Stripping membranes with biotinylated proteins Patrice Boissy
SpinBind Oliver S. DePeyer
Protocol for Re-using of Qiagen tips Trevor Bezdek
Bmh71-18 strain lperal
cDNA library kits-your choice Andy Phillips
DNA precipitation? steve johnson
Pyrimidine Dimers Brett Beitzel
Q: pRK5 vector sequence? AB
DNA precipitation? Gregory S. Buzard 'Buz'
GeneClean II NaI conc??? Chip Peele
Methods and Reagents FAQ list Paul N Hengen
Prok. expression vectors; anything that is not based on Lac operon and Amp R? Peter Wang
Glycoprotein Detection Iain Wilson
direct blotting system LMordo
R.E.D. problems Andrew Hniat
Clontech Laboratories is a BIOSCI sponsor!! BIOSCI Administrator
Need help with Qiagen PB Buffer? Charles A Miller
Gibco WWW site Chuck Sohaskey
Inverse PCR vilimf01 at mcrcr6.med.nyu.edu
RT of G-cap into C? vilimf01 at mcrcr6.med.nyu.edu
Protocol for RT-PCR in shrimp Tim F. Carl
Gibco's WWW site? Horst Ibelgaufts
single band from rRNA from brachionus sp. (Dave Johnston)
UDP preparation shokhen
Shading Functional Domains Neil Harris
Internalization of the protein into cultured cells Yoo Hun Suh
Robot Cycler Ted Michelini
Degenerate primers for PCR Ted Michelini
Purifying clean plasmid vectors for ligation Dr. Duncan Clark
Expression of GST fusion Petri Kursula
Gibco WWW site Horst Ibelgaufts
Agarose and preparative gels... Anthony Tomlinson
cracked gels? Helen Korsmo
RNA extraction-whole blood Ian Graham
Hsp70 Expression Vectors samueh01 at mcrcr6.med.nyu.edu
Distinct bands in restriction dig. of genomic DNA Graham Dellaire
direct blotting system Keld Sorensen
Gibco's WWW site? R. John Lye
Storage of serum in frost-free freezers? R. John Lye
Does TK work in Neuro-2a cells? Matthew P. Frosch, M.D., Ph.D.
What size DNA stays in soln when NH4AC ppt? John Dixon
single band from rRNA from brachionus sp. Joseph C. Bagshaw
Isolation of nulcei Kristina Warton
SpinBind MAN!!!
Zonal gradients Dr E. Buxbaum
How stable biotin-avidin? CPGYIELD
low recovery with PromegaPolyATtract kit ? CPGYIELD
Primer Design sites Ed Castro
Immuno(histo)fluorescence: what's the best method? Dr E. Buxbaum
sequencing murine p53 (fwd) Ashraf R. Dallol
cDNA-Library of monocytes melkony at wwupop.uni-muenster.de
Gibco's WWW site? Annette
Genetic Meeting Jena Johannes Woestemeyer
mRNA Differential Display Harm Deckers
Best chemiluminescence for W.blotting tera
Gibco's WWW site? john brennand
Glutathione redox buffer? Csaba Jeney
anti 5-lipoxygenase antibodies fred wouters
NdeI: cutting problems?? anyone else?? seems silly! (fwd) Rich Kernan
Large scale low melt extraction J. Pat Martinez
Degenerate primers for PCR Mr. Chris Hoyle
pAX Fusion Protein Vectors Krish Sakhamuru
How stable biotin-avidin? Paul N Hengen
Protein inhibitor(s) during immunoprecipitation ? Dr. P.A. de Zoysa
Storage of serum in frost-free freezers? Jfloring at aol.com
TA cloning Jose M. Bautista
Lambda exonuclease Robin Beech
cracked gels? Curt Ashendel
Primer Design Xiu Yu
What size DNA stays in soln when NH4AC ppt? Riekeltje Koedood
Yolky DNA s060965 at AIX1.UOTTAWA.CA
disrupting filimentous fungi Andrew Hopkins
NdeI: cutting problems?? anyone else?? seems silly! jovermeyer at shrsys.hslc.org
Inverse PCR jovermeyer at shrsys.hslc.org
A question about in situ hybridization Paul N Hengen
Inoue Competent Cell procedure Christian Moews
COS-cells Christian Moews
Mutagenesis kits Rick Neubig
Gibco's WWW site? Peter Hunziker
DNA precipitation? daniel morris
NdeI: cutting problems?? anyone else?? seems silly! (fwd) daniel morris
PCR through GC-rich sreesh
Re-post: PCR through GC-rich sreesh
PCR through GC-rich Dale Levitzke
Gibco Site Patrick HJ Falckh
PCR through GC-rich Dale Levitzke
disrupting filimentous fungi Dima Klenchin
RT of G-cap into C? Anton Scott Goustin
Inoue Competent Cell procedure Ned Mantei
Yolky DNA vilimf01 at mcrcr6.med.nyu.edu
pAX Fusion Protein Vectors Joao Vasconcelos Costa
Peptide synthesis Iain Crowder
Transcription Factors Sergey V. Orlov
anti 5-lipoxygenase antibodies Sailesh Surapureddi
what is a Far South-Western blot? Horst Ibelgaufts
SpinBind Oliver S. DePeyer
some information about hormones needed... Sergey V. Orlov
Ab to inositol monophosphatase Michael Kane
Protein inhibitor(s) during immunoprecipitation ? Mark R. Miglarese
Bmh71-18 strain Mark R. Miglarese
Free Sulphydryl detection Michael Daws
Flow Cytometer Surasakdi Wongratanacheewin
cracked gels? Eddie Diehl
mouse lymphotoxin David Knight
FloriGen newsgroup keyword search Bill McKendree
PCR: How to remove DNA contamination from the starting mRNA? Simon Golding
20% gel drying Laigle
Plasmid Drawing Program MARK PEGGIE
PCR from saliva? Igor R Sagdeev
Light Sensitivity of Tris Per Bergman
Bmh71-18 strain Michael Benedik
PCR contamination-HELP! Warren D. Kruger
fidelity of reverse transcriptase Regina Shaw
Primer design (Oligo) Benny Shomer
in-situ hyb in lung: method of fixation? Gil
Help: Lambda purification kits Ulrich Harttig
FBS users PLEASE READ - IMPORTANT martin LEACH
PCR: How to remove DNA contamination from the starting mRNA? Mark R. Miglarese
PCR amplification of mouse kappa chains H. David Fischer
Bmh71-18 strain Karl Fischer
NdeI: cutting problems?? anyone else?? seems silly! (fwd) Bernard Murray
RNA isolation/Chomozynski Susan Patrick
RNA isolation/Chomozynski Susan Patrick
RNA isolation/Chomozynski Susan Patrick
Methylen blue DNA staining Paul N Hengen
methylation of DNA in mice mrtour at stud.med.cornell.edu
FBS users PLEASE READ - IMPORTANT Keld Sorensen
longggg mRNA transcript benan dincturk
Agarose and preparative gels... HIRANYA ROYCHOWDHURY
Spinach library benan dincturk
Free Sulphydryl detection Keld Sorensen
Reuse of Qiagen Ni-NTA Spin columns?? Bradley Turner
Membrane properties Achim Recktenwald
PCR HELP! DXS52, Xq28 (St14 VNTR) anybody with experience? John Wolff
Wizard PCR purification from agarose gel CLAREN321
Northern hybridizations bbraun at ucla.edu
Testis library for two-Hybrid?? Jon Nakamoto
Lipase assay ZOOMmmm
PCR HELP! DXS52, Xq28 (St14 VNTR) anybody with experience? Dr G R Taylor
2D-Software Harry Reis
Help: Rapid determination of copy No. of a gene in bacterial chromosome. gwade at physiology.uwo.ca
Northern hybridizations logan
Sequencing Gel Rigs for SSCP Bernhard
PCR AMPLIFICATION OF MOUSE KAPPA CHAINS Andrei Popov
inosine primers Tm?? eric anderson
phage display using Stratagene's Surfzap Kit Alex Chang
The KlenTaq mystery Ted Michelini
Gibco's WWW site? Roger Baird
Circular Dichroism r.jackson at ic.ac.uk
SOS!--PCR restriction cut Wenjin Yu
seeking protocal for RNase assay by gel electrophoresis Peter
Addendum: Sequencing Gel Rigs for SSCP Bernhard Mayr
Addendum: Sequencing Gel Rigs for SSCP Bernhard Mayr
2D-Software WSchick at aol.com
Boomerang DNA Amplification Web Page Paul N Hengen
Wizard PCR purification from agarose gel Colin Rasmussen
Looking for UV-DNA endonuclease Michael Adamson
Re-post: PCR through GC-rich Dale Levitzke
PCR from saliva? Adrian.Philbey at SMTPGWY.AGRIC.NSW.GOV.AU
RNA isolation/Chomozynski Patrick HJ Falckh
PCR contamination-HELP! Harry J. Witchel
Gibco's WWW site? john brennand
Help: Lambda purification kits john brennand
enantiomeric amino acids Michael Moormann
SOS!--PCR restriction cut Ashok Aiyar
Best chemiluminescence for W.blotting Keld Sorensen
Best chemiluminescence for W.blotting Keld Sorensen
cracked gels? Darren Boehning
Exogenous ATP supply - help requested Neil Winegarden
Best chemiluminescence for W.blotting Jen Logan
What is Touchdown PCR? spyong at pc.jaring.my
Lipase assay "Marianne Leverone ", BIO
long mRNAs benan dincturk
RE- PCR Contamination Brett Beitzel
Pumps for Zonal Rotors Juergen Brosius
Antibodies against inclusion body proteins? Simon Dawson
PCR ampl. of a variable locus Juergen Brosius
anti 5-lipoxygenase antibodies Dr. Stephen Hoare
phage display using Stratagene's Surfzap Kit tera
Isolation of nulcei Dr E. Buxbaum
SOS!--PCR restriction cut Edgar Valencia
What is Touchdown PCR? Craig Echt
cracked gels? Dr E. Buxbaum
Protein inhibitor(s) during immunoprecipitation ? Dr. Stephen Hoare
Mycoplasma detection kits, and mycoplasma effects Ian A. York
PCR mutagenesis and proofreading polymerase prscholl
P.aeruginosa PAO-1 "Marianne Leverone ", BIO
PCR mutagenesis and proofreading polymerase Margon Vandongen
What is Touchdown PCR? Paul N Hengen
PCR stutters through oligo-A regions Juergen Brosius
SOS!--PCR restriction cut C.L.L.
Sequence-primer for lambda Pl? Troels Wind
Rainin edp plus Lesley Weston
Source of Proteoglycans? Bradley Turner
cracked gels? Bart Sefton
Green Fluorescent Protein Kent Hill
how to incubate Ab/membrane? J Quyen Wickham
PCR stutters through oligo-A regions logan
longggg mRNA transcript Konstantin M. Chumakov
Best chemiluminescence for W.blotting Bernard Murray
Shrimp virus assay Joseph C. Bagshaw
Wizard PCR purification from agarose gel Song-Kun Shyue
5' RACE vilimf01 at mcrcr6.med.nyu.edu
PCR mutagenesis and proofreading polymerase upyers
PCR stutters through oligo-A regions Jeffrey D. Bray
Slow dye for sequencing gels? Stephen Morris
PCR contamination-HELP! vilimf01 at mcrcr6.med.nyu.edu
cracked gels? Pascal_Bochet
Gene clean reagent list. Ron Sayler
PCR stutters through oligo-A regions vilimf01 at mcrcr6.med.nyu.edu
Heat lability of DEPC simon
SpinBind A.S.
phage display using Stratagene's Surfzap Kit Alex Chang
A260 or Fluoromete ??? Which is correct Shaun Tyler
cracked gels? Riekeltje Koedood
SOS!--PCR restriction cut ProZyme
TNT kit with T7 RNA Pol HONG
silver stain LAU Tze Chin
Green Fluorescent Protein Purification Short Courses Daniel Gonzalez
What is Touchdown PCR? NOT IDAHO WSchick at aol.com
Green Fluorescent Protein WSchick at aol.com
Pumps for Zonal Rotors WSchick at aol.com
Site directed mutagenesis kits -recommend? Sonya Clark
Protein inhibitor(s) during immunoprecipitation ? Bernard Murray
isolation of small DNA fragments CDMENU at ccs1.cc.monash.edu.au
Gene clean reagent list. Simon Dawson
Peptide SLIGRL Ferland Louis H.
Green Fluorescent Protein A.Doherty
Green Fluorescent Protein A.Doherty
PCR contamination Neil Burns
Qiagen RNeasy: Early precipitate?? Jim Hill
Any commercially available plasmids with HA, myc or T antigen tags. Obaid Y. Khan
Site directed mutagenesis kits -recommend? john brennand
Site directed mutagenesis kits -recommend? Mark R. Miglarese
Solaris (Unix) on IntelPC Don Gilbert
Blocked Xba I sites? Todd Miller - Pharmacology
SOS!--PCR restriction cut Bob Horton
longggg mRNA transcript Bob Horton
transfection standard Soeren Jensby Nielsen
Transfection of lymphoid leukemia cell lines Richard Moldwin
SolarisX86 Bradley Turner
Acetylcholinesterase (AChE) regeneration after DFP? Alan
A260 or Fluoromete ??? Which is correct john brennand
Acetylcholinesterase (AChE) regeneration after DFP? T. Chyau Liang
phage display using Stratagene's Surfzap Kit tera
Anyone heard of pCMV7? KPH
Exogenous ATP supply - help requested john brennand
Mutagenesis kits Elizabeth Rogers
T4 ligase clone Scott Vande Pol
buy GP120 peptide? Russ Boggs
chickens vs rabbits for antibodies Scott Vande Pol
Site directed mutagenesis kits -recommend? tera
oligo annealing/cloning problem Robert F. Leclerc
Expression vector tags RMMMS at CUNYVM.CUNY.EDU
Hsp70 Expression Vectors RMMMS at CUNYVM.CUNY.EDU
isolation of small DNA fragments Duke Groebe
PCR stutters through oligo-A regions Juergen Brosius
messy agarose bands J.C.
? Re: Representational Difference Analysis Teresa Shippy
RNase protections assays. Too much salt? Angela Hofstra
PCR: How to remove DNA contamination from the starting mRNA? QIAGEN
Acetylcholinesterase (AChE) regeneration after DFP? Achim Treumann
Expression vector tags Jon Hobman
RNA isolation/Chomozynski Daniel Pereira
Rainin edp plus Michael Bausher
IFN-treated cell cDNA library Seng-Lai Tan
Free Sulphydryl detection Achim Recktenwald
Expression vector tags John Elliott
Expression vector tags John Elliott
messy agarose bands John Elliott
Rainin edp plus batteries davesmith at bioch.tamu.edu
Touchdown PCR Joao Vasconcelos Costa
Protocol for Re-using of Qiagen tips Ferland Louis H.
chickens vs rabbits for antibodies David Martin
SOS!--PCR restriction cut Dr. Duncan Clark
Green Fluorescent Protein Dr. Duncan Clark
messy agarose bands john brennand
transcription factor binding sites software? Eckhard Boles
A260 or Fluoromete ??? Which is correct WSchick at aol.com
influenza hemagglutinin abs Chris Boyd
PCR-cloning P. Mertens
tips for blunt ended ligation! Chris Boyd
A260 or Fluoromete ??? Which is correct john brennand
silver stain Michael West
ELISA reagent supplier help needed! Dr.G.R.Rajasekariah
ELISA reagent supplier help needed! Dr.G.R.Rajasekariah
Media Question: What is Standard-1 from E. Merck? Paul N. Brothers
SOS!--PCR restriction cut Kiley R. Prilliman
POSITION AVAILABLE: Research Asst. Grtful ned
2D-Software eqpcmv at univ-lille1.fr
2D-Software eqpcmv at univ-lille1.fr
FBS users PLEASE READ - IMPORTANT martin LEACH
Req: Electroporation of myloid and megakaryocyte cell lines, HELP?? Carmelo Volpe
RE- Touchdown PCR Brett Beitzel
ALF-express Toni Whistler
? differntial PCR jms5 at Lehigh.EDU
Free Sulphydryl detection "Marianne Leverone ", BIO
Teicoplanin antibiotic (where can I get it) Al
expression vector tags Tony Sanchez
Touchdown PCR Paul N Hengen
A260 or Fluoromete ??? Which is correct Bradley Turner
Qiagen RNeasy: Early precipitate?? QIAGEN
Wizard PCR purification from agarose gel Don Rollins
SpinBind and Supelco Oliver S. DePeyer
Need help with Qiagen PB Buffer? QIAGEN
Gibco's WWW site? martin LEACH
req. opinions of Ericomp thermocycler Don Rollins
chickens vs rabbits for antibodies L. Fliegel
In vivo expression vectors Job Tharappel
Primer extention analysis by PCR Toshio
PCR on cells in suspension Tony Sanchez
Wanted: tk promoter - b gal construct Changcun Jia
ß-Amyloid peptide solubility Joaquin Arribas
Anybody working with VEGF? tick
Site directed mutagenesis kits -recommend? at
Reply: DN Ligation Andrea Krempler
PCR stutters through oligo-A regions Pamela Norton
pAX Fusion Protein Vectors Torsten Boerchers
Colourimetric CAT assay? Oksana K. Yarosh
5' flourescein labelling and ABI, anyone tried it
RNase protections assays. Too much salt? Pamela Norton
transformation problems Dr. Paul N. Brothers
How does pH effect Terrific Broth? Charles A Miller
Site directed mutagenesis kits -recommend? Dr. Paul N. Brothers
Ethidium bromide stained acrylamide gels for microsatellites Rick Johns
Primer extention analysis by PCR Ken Howe
small PCR product-->ligation luis
req. opinions of Ericomp thermocycler lonnie
Primer extention analysis by PCR Russell L Wrobel
PCR contamination-HELP! David Hosfield
How does pH effect Terrific Broth? Ted Michelini
A260 or Fluoromete ??? References WSchick at aol.com
Ethidium bromide stained acrylamide gels for microsatellites Ned Mantei
Site directed mutagenesis kits -recommend? Eckhard Boles
Free Sulphydryl detection David Martin
PCR in Nepal Dr. J.P. Clewley
RNA isolation/Chomozynski Ferland Louis H.
Protein purification Inmaculada Garcia
Info about Auto Sequencer...! pramod at nii.ernet.in
HIV p24 Antigen Administrador del nodo
Gibco's WWW site? john brennand
HEX-labelled primer doesn't work in PCR ? Shirley Horn-Saban
Protein purification Jose M. Bautista
Polyacrylamide gel fixation for DNA David R. Johnson
Wanted: tk promoter - b gal construct helix.nih.gov
small PCR product-->ligation Gary Kucera
Inoue competent cell procedure Arfaan Rampersaud
disrupting filimentous fungi William Moens
Need to find source of teicoplanin Al
Need to find source of teicoplanin Al
Anyone heard of pCMV7? (fwd) Jan Ulrik Thorup
chickens vs rabbits for antibodies Richard Van Frank
Anyone heard of pCMV7? Michael Daws
anti dsDNA latex agglutination Rafel Ferragut
Hsp70 Expression Vectors Seng-Lai Tan
Reply: DN Ligation Paul N Hengen
TROPIX Dual-LIght P. Vijay Sarthy
Non-rad. Northerns Scott Selby Med-Hem
Primer extention analysis by PCR Paul N Hengen
PCR contamination-HELP! Riekeltje Koedood
Kan resistance and pH Rafael Maldonado
Anyone using the ABI Catalyst 800 for genotyping? Don Bowden
ALPHA ANNOUNCES NEW PROCEDURES TO ENHANCE PLASMA PRODUCT SAFETY John Gross
Extra Long PCR reamplification Konstantin M. Chumakov
Strep fusion protein vector? William_P_Prichett
Reply: DN Ligation David Micklem
HELP! GST column with Tris-Saline? sujoy ghosh
Taq Extender--degrading A overhangs! Jeffrey Shane Temple
NdeI: cutting problems?? anyone else?? seems silly! (fwd) Arioch
PCR contamination Shaun Tyler
Modified pGEM? Angel Yanagihara
Any mass spec for protein sequencing under $30,000? XZHENG at GAMMA.IS.TCU.EDU
LI-COR, Inc. sponsors BIOSCI! BIOSCI Administrator
PCR stutters through oligo-A regions Juergen Brosius
Where can obtain AMVN .... Depto. Biologia Celular
Inoue competent cell procedure Rico Laage
Non-rad. Northerns Rico Laage
SpinBind and Supelco Jasper Saris
e-mail Piatak Administrador del nodo
PCR can not get intense bands P. Mertens
Expression vector tags Kiley R. Prilliman
RNA mass ladder greater than 10kb Martin Collinson
GST Fusion stuck on sepharose John
Fog in In Vitro Translations John
pcDV1 sequence mpoulin at s-and-s.com
Inoue competent cell procedure john brennand
RNA mass ladder greater than 10kb Steven Tait
5'-> 3' Yegor Vassetzky
PCR can not get intense bands KHUANG at CHEMVX.TAMU.EDU
Fragment prep. using QiaTip? QIAGEN
yeast transformation efficiency Ray Lu
Inoue competent cell procedure Bradley Turner
Inoue competent cell procedure Bradley Turner
PCR can not get intense bands HIRANYA ROYCHOWDHURY
Any mass spec for protein sequencing under $30,000? Eddie Diehl
Structural Biochemistry Jose M. Bautista
Extra Long PCR reamplification upyers
Modified pGEM? S John
calyculin A Annick Breton
isolation of small DNA fragments Harry J. Witchel
Custom Antibodies Gustavo Nolasco
Techniques to study genetic drift (query) Ravi Menon
Inoue competent cell procedure Paul N Hengen
PCR can not get intense bands Bernard Murray
Fog in In Vitro Translations Lee Weber
USB t4 DNA ligase ----PROBLEMS? teb3 at Lehigh.EDU
Primer extension by rTth Reverse transcriptase ? Toshio
HELP! GST column with Tris-Saline? Michael DiDonato
Site directed mutagenesis kits -recommend? Margon Vandongen
storage of E.coli Administrador del nodo
Protocol for Re-using of Qiagen tips QIAGEN
Transfection of HL60 Robert Slany
isolation of small DNA fragments Jeff Smerage
Fog in In Vitro Translations Arthur Wohlwill
magnetophoretic systems lonnie
PCR can not get intense bands Zahra Fayazi
PCR can not get intense bands Zahra Fayazi
Looking for a histochemical ATPase protocol... Jil Tardiff
How does pH effect Terrific Broth? asdas
Inoue Competent Cells Amna Saeed
The Nest Group, Inc. sponsors BIOSCI! BIOSCI Administrator
Homemade Shaking Incubator - decode pictures Robert Horton
(none) Administrador de iihema.fmed.uba.ar
(none) Administrador de iihema.fmed.uba.ar
Help: looking for a source for phytochelatin. Jin-Hong Kim
Looking for a histochemical ATPase protocol... Matthew P. Frosch, M.D., Ph.D.
PCR can not get intense bands Dr G R Taylor
PCR can not get intense bands Dr G R Taylor
substractive libraries: suggestions ?? Jean-Yves Masson
Heterologous probe a problem in Southern spyong at pc.jaring.my
transcription factor binding sites software? Bob Horton
Gibco's WWW site? Roger Baird
chickens vs rabbits for antibodies Robert Randolph
Taq Extender--degrading A overhangs! Margon Vandongen
Reply: DN Ligation Pamela Norton
Fog in In Vitro Translations Michael Morales
Seeking Proteoglycan Source Bradley Turner
Reply: DN Ligation UV Filter WSchick at aol.com
Antibody(ies) to Rat Aldolase(s)..........anybody? David Woodcock
What size DNA stays in soln when NH4AC ppt? Xiaohui Ji
IMPORTANT - BIOSCI Fundraising Update! BIOSCI Administrator
Protocol for Re-using of Qiagen tips Ferland Louis H.
Boomerang, Vector-Splinker ette: Any success? Juergen Brosius
southern blotting of small fragments Liam E Good
Tyramide Signal Amplification mark throsby
cracked gels? Dima Klenchin
cracked gels? albano renee
semi-blotting Liam E Good
yeast transformation efficiency S.L. Rennie BSc, Lab Manager
Taq Extender--degrading A overhangs! vilimf01 at mcrcr6.med.nyu.edu
Tyramide Signal Amplification Keld Sorensen
glycogen as a carrier Ari Kahn
Primer extension by rTth Reverse transcriptase ? phillip buckhaults
Tyramide Signal Amplification Jerry Litt
Detection of molecular weight marker bands on +tively charged membranes labimap
Protocol for Re-using of Qiagen tips Harry J. Witchel
Gibco's WWW site? john brennand
subscribe Don Faller
PCR Lyme Disease (DNA extraction protocol from serum) Paulo Santos
porin production in E. coli - advice needed Jean-Yves Paquet
Trial sending Osama
PCR contamination Osama
HELP : Dosage of biotinylated proteins Patrice Boissy
how to get rid of ethidium bromide??? Arioch
CNBr and phosphate groups Randall Willis
antibodies to tetM Laura Conway
Luminimetry with luciferase: wavelength please! I.R. Franklin
Re- Isolation of small DNA Brett Beitzel
Re- PCR contamination Brett Beitzel
concentration of biotinylated proteins Keld Sorensen
Alternative to autoclaving for denucleasing plasticware zeus
DMSO and bacteriophage Helen Logan
ANTI-RAT ANTIBODIES Chris Regan
Post-doc needed Jay L. Rothstein
Heterologous probe a problem in Southern Steven Goldberg
Info about Auto Sequencer...! Steven Goldberg
porin production in E. coli - advice needed Dilip Dias
POST DOC AVAILABLE gabre
storing gels... Arioch
oligo annealing/cloning problem Daniel Pereira
semi-dry elecrophoretic xfer Vs wet e.phoretic xfer ... Arioch
RNase protections assays. Too much salt? Daniel Pereira
DNA binding Protein Inhibition Tina Hallis
Radiolabelled phosphoinositols Jon Church
German drug screening, assay, metabolism vendors? Philip W. Stephenson
porin production in E. coli - advice needed Dilip Dias
porin production in E. coli - advice needed Dilip Dias
Northern blots without formaldehyde Doug Johnson
are introns neccessary for transgene expression Liz Mann
Siliconized coverslips for In Situ Hybridization wgarrett at GGPL.ARSUSDA.GOV
are introns neccessary for transgene expression milstone
Antibodies against dermatophytes Steffen Roth
Excision of LambdaZap Products Robert A. Schoenfeld
Scintillation Counting Without Scintillation Fluid Philip P Calvert
cracked gels? ProZyme
Detection of molecular weight marker bands on +tively charged membranes ProZyme
Help about blood storage please! Jimmy Rail
are introns neccessary for transgene expression Michael Morales
PCR contamination-HELP! Morrison Lab
glycogen as a carrier Colin Rasmussen
Help: Ribonuclease protection assays. Married with Children
Northern blots without formaldehyde Amie Franklin
Alternative to autoclaving for denucleasing plasticware Ned Mantei
Gibco's WWW site restriction on server? Microplate at eworld.com
Help : Hygromycin dose for Daudi kim myung jong
Alternative to autoclaving for denucleasing plasticware Microplate at eworld.com
SDS in western blot transfert Lionel Berthoux
20% gel drying Kaj Stenberg
Excision of LambdaZap Products Alexander Kraev
glycogen as a carrier John Dixon
Purifying clean plasmid vectors for ligation Steven Goldberg
Looking for a histochemical ATPase protocol... H.Hoppeler
Looking for a histochemical ATPase protocol... H.Hoppeler
SDS in western blot transfert Dima Klenchin
Excision of LambdaZap Products Jon Stewart
PCR-primer with T7 Promotor for TK Friedemann Weber
commercial adenovirus vectors bezstarosti at bc1.fgg.eur.nl
Gel documentation system K. Wernars
SDS in western blot transfert Michael Bausher
DNA binding Protein Inhibition Duke Groebe
HELP! GST column with Tris-Saline? Cornelius Krasel
info on HPLC and related techniques Marc Visconti
p53's apoptotic function mrtour at stud.med.cornell.edu
storing gels... Monica L. Parker
DMSO and bacteriophage john brennand
Siliconized coverslips for In Situ Hybridization Gabriele Kerber
PCR in Nepal Bob Horton
Genetically Directed RDA? Teresa Shippy
GST Fusion stuck on sepharose Simon Dawson
Stripping Streptavidin-HRP Michael Daws
Stripping Streptavidin-HRP Michael Daws
Luminimetry with luciferase: wavelength please! Glenn A. Warr
GST Fusion stuck on sepharose David Roof
Immunohistochemistry: Technique Question Auditorium
pZErO plasmid George Clark
Green Fluorescent Protein L. Fliegel
Positive control for PTK probe Tracy E. Anderson
Detection of molecular weight marker bands on +tively charged membranes Ed Stephenson
Help needed for DNase I Footprinting Dominic Voon
Automated sequencing reactions phillip buckhaults
Post-doc needed Klaus Salger
Northern blots without formaldehyde Klaus Salger
Automated sequencing reactions mikel at vaxc.cc.monash.edu.au
Immunohistochemistry: Technique Question Jerry Clayton
**Anyone have a murine Interferon gamma expressing plasmid or cassette??*** Dr Mark Watson
pZErO plasmid Richard Daines
Help needed for DNase I Footprinting Ferland Louis H.
PCR in Nepal WSchick at aol.com
Hsp70 Expression Vectors Bradley Turner
Help about blood storage please! P. Mertens
info about NTCB Patrick Van
PC version of FASTA vipond at BSA.BRIS.AC.UK
List of Genome projects david johnston
Seeking "Bioconsumer Review" Bradley Turner
info about NTCB Krishnaswamy
Vascular Endothelial and Smooth Muscle Cells John Fox
Antibodies required..badly! Ashraf R. Dallol
Transforming bacteria for protein production Patrick Grealish
Is there a FAQ for this group? Patrick Grealish
Siliconized coverslips for In Situ Hybridization ingen at ingenlabs.com
Has anyone done NF1 (The transcription factor) Northerns? EILEEN
Seeking "Bioconsumer Review" FREE WSchick at aol.com
Seeking "Bioconsumer Review" Keld Sorensen
Re- pZErO Brett Beitzel
In situ hybridisation GILLIAN NI BHARLO BOTANY PG
Hoeffer Progenetor II electroporation unit Robert Coelen
Is there a FAQ for this group? Paul N Hengen
Help about blood storage please! Dave Hostler
p53's apoptotic function Jean-Philip Truman
DNA binding Protein Inhibition Pamela Norton
Thanks for tips for GC-rich PCR! MAN!!!
Differential Display PCRs - help needed! Miss J.M. Cameron
chylomicron retention Karl G. Kohlgraf
Repetitive Northern Hybridization Gabriel Romero
p53's apoptotic function Bob Scibienski
Linear acrylamide (was --Re: glycogen as a carrier) Glen Shearer
Northern blots without formaldehyde Glen Shearer
CNBr and phosphate groups Bart Sefton
Mycoplasma detection kits, and mycoplasma effects Bradley Turner
Getting cells off plates Pearse Ward
Need to remove nucleic acids from nuclear extracts ##Sophie## ##Candon##
free sequence homology search on WWW Jane O'Brien
Getting cells off plates Edward Wang
Phage DNA Isolation KHUANG at CHEMVX.TAMU.EDU
EtBr optimal emission spectra Torsten Karlsson
Getting cells off plates John Fox
Mycoplasma detection kits, and mycoplasma effects A.Doherty
RNA isolation- the long and the short of it... przemko
PCR through GC-rich Holger Steinlechner
yeast transformation efficiency ZYMO RESEARCH
Taq Extender--degrading A overhangs! Holger Steinlechner
Site-directed PCR mutagenesis Hans Kiefer
glycogen as a carrier Simon Dawson
Transforming bacteria for protein production Vanhauwe Jurgen
tuning of expression levels Marcel Hoffmann
Scintillation Counting Without Scintillation Fluid john brennand
Phage DNA Isolation john brennand
Getting cells off plates Dima Klenchin
source for ganciclovir Dima Klenchin
source for ganciclovir Lyn A. Thet
Re- pZErO Steven Goldberg
EcoRI adaptors Jane Q. Employee
Help primer extention analysis Toshio
SOS Call to all experinced "Sequencers" spyong at pc.jaring.my
LCR (Ligase Chain Reaction) kit Abraham Rosenberg
Expand PCR and TA cloning kits? John Dixon
Differential Display PCRs - help needed! Louis Alarcon
RNA isolation- the long and the short of it... Shelley Cole
how to get rid of ethidium bromide??? Brian M. Shewchuk
RNase protections assays. Too much salt? Brian M. Shewchuk
How to put a phosphate group on 3'OH of ssDNA? Robert SCHROCK
pZErO plasmid Arnoud Kal
Zonal Rotor vibrations craig gatto
TOL Plasmid Prep - protocol K.G. Korth
Expand PCR and TA cloning kits? Margon Vandongen
SOS Call to all experinced "Sequencers" Michael DiDonato
Help with Transcription Factors Database? Jimmy Rail
Plant Expression Vectors Dr. Randal W. Giroux
Expand PCR and TA cloning kits? tera
rxn of protein a with denatured ab ProZyme
Getting cells off plates simon
good sequencing unit christine-elsik at ansc.tamu.edu
RNase protections assays. Too much salt? CAShug
Plant Expression Vectors Masashi Ugaki
Help: Ribonuclease protection assays. CAShug
Drying sequencing gels John Rebers
Isolation of nulcei s1050589 at iplabs.ins.gu.edu.au
Isolation of nulcei s1050589 at iplabs.ins.gu.edu.au
Help about blood storage please! Dave Hostler
Polymerase fidelity and KlenTaq suppliers Brian Shipley
Getting cells off plates Tom Vink
Site-directed PCR mutagenesis Philippe Lefebvre
info on HPLC and related techniques John Elliott
What is "Cleavase" Raymond Dalgleish
GIBCO:Gene-trapper Kit Tegelk at ear002.uni-muenster.de
Pellet paint ? john brennand
Virtual Reality in SYBR Space Wayne Coco
DNA base composition Joseph C. Bagshaw
p53's apoptotic function CSJGH at leeds.ac.uk
Peptide Nucleic Acids (PNA) Mick Jones
Anyone heard of this company? Marc Visconti
how to get rid of ethidium bromide??? Bob Horton
EcoRI adaptors john brennand
Cutting Qiagen miniprebs?? Bjoern Voldborg
A Budded Yeast: One or Two? Namjin Chung
Linear acrylamide (was --Re: glycogen as a carrier) john brennand
EcoRI adaptors James R. Miller
Help about blood storage please! mikem at s-and-s.com
Yeast Knockout? Namjin Chung
SYBR Reply STEVEN GERHARDT
Drying sequencing gels HIRANYA ROYCHOWDHURY
RNA Oligo supplier??? John Storring
TNT kit with T7 RNA Pol Hans Bernd Prisack
TNT kit with T7 RNA Pol Hans Bernd Prisack
Drying sequencing gels Anthony Tomlinson
how to get rid of ethidium bromide??? Anthony Tomlinson
SOS Call to all experinced "Sequencers" Anthony Tomlinson
Linear acrylamide (was --Re: glycogen as a carrier) David Grant
cDNA molecular wieghts GILLIAN NI BHARLO BOTANY PG
Announcement: 1996 Cold Spring Harbor Lab Manual Source Book nw available Joan Boyce
Pellet paint ? Paul N Hengen
Help with Transcription Factors Database? username at bay.cc.kcl.ac.uk
TNT kit with T7 RNA Pol Margon Vandongen
Commercial nonradioactive riboprobes Paul C. Grimm MD
PCR hood Bob Kodrzycki
What is "Cleavase" Dr G R Taylor
Opinions on CapFinder? John Finnerty
Use of d(AT)9 probe for detection of microsatellites Marcel Fernandez
Wanted: Peptide quantitation method W.H. Colledge
Any mass spec for protein sequencing under $30,000? asdas
Do you know? Tess
Pellet paint ? Walter Hill
(none) Administrador de iihema.fmed.uba.ar
gal4 strains for yeast two-hybrid Seth Corey
Getting cells off plates L. Bastian
Culture of E.Coli (SURE) / Large Plasmids / Foreign Sequences Dr Alexander J Annala
What's behind the AmpliTaq Gold trick? Zophonias O. Jonsson
cDNA library..help CRH
TNT kit with T7 RNA Pol Kojo Mensa-Wilmot
Screening for DNA binding protein Rich Kernan
TNT kit with T7 RNA Pol Jen Logan
Getting cells off plates Mike Gruidl
Getting cells off plates Jen Logan
How to gel PEG solutions? Terry Conley
Luciferase in Vero problems David N. Levy
Cutting Qiagen miniprebs?? Richard Daines
streptolysin experts wanted Darren D. Browning
Getting cells off plates Microplate at eworld.com
Alternative/autoclaving for denucleasing plasticware STOP Microplate at eworld.com
Transfection of HL60 L. Bastian
plasmid cDNA library amplification CartonKJ
PFGE references? Anonymous
How to gel PEG solutions? Ted M.
Pellet paint ? Ted M.
good sequencing unit Ted M.
plasmid cDNA library amplification TJ Murphy
free sequence homology search on WWW Juergen Brosius
RNase Protection Assay hint.. Osama
Assay of vitamins Krzysztof Treder
cDNA library..help Andre Renard
plasmid cDNA library amplification Frances Hannan Zoology
Need to remove nucleic acids from nuclear extracts joestudent at mit.edu
How to get a free copy of the 1996 CSHL Source Book JoanBoyce at aol.com
Recombinant phage antibody system Chao-Liang Wu, PhD
PFGE references? WSchick at aol.com
TNT kit with T7 RNA Pol Jennifer Lyon

Last message date: Sun Mar 31 23:35:03 EST 1996
Archived on: Sun Mar 6 20:59:58 EST 2005

Messages sorted by: [ thread ] [ subject ] [ author ] Index

This archive was generated by Pipermail 0.09 (Mailman edition).