Methods March 1996 Archives by thread

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Starting: Fri Mar 1 01:28:18 EST 1996
Ending: Sun Mar 31 23:35:03 EST 1996
Messages: 1286

Alkaline gels-Better method? Amie Franklin
nicking DNA plasmids on purpose Amie Franklin
- nicking DNA plasmids on purpose Zophonias O. Jonsson
  - nicking DNA plasmids on purpose Mark Walberg
    - nicking DNA plasmids on purpose Ned Mantei
Density gradient cell separation and streptavidin-agarose? G.P.
FIAU-Where can I buy it? Georg Weitzer
Non-detergent containing lysis buffer for primary cell culture? G.P.
- Non-detergent containing lysis buffer for primary cell culture? John K. Troyer, PhD.
G418 Mike
- G418 Madhu Singh
- G418 Henry Hoppe
HELP! GST column with Tris-Saline? Cornelius Krasel
LexA antibodies? Ian Graham
Deuterium oxide in density gradient? Dr E. Buxbaum
HELP! looking for N-ras intronic sequence. Erwin Pauws & Carrie Ris
freeze-squeeze Dr. K.Hoare
Staining DNA in Agarose with Methylene Blue Joseph C. Bagshaw
Alkaline Phosphatase substrates Gabriele Kerber
pHOOK (CaptureTec system) Graham Atherton
DNA sequence for rat lcwilliams at medusa.unm.edu
- DNA sequence for rat jms93
Amersham Hybond N problem BRITTA SCHULZ
- Amersham Hybond N problem Alexander Kraev
- Amersham Hybond N problem Paul N Hengen
  - Amersham Hybond N problem Tori
- Amersham Hybond N problem vilimf01 at mcrcr6.med.nyu.edu
  - Amersham Hybond N problem Cathy Sprankle
  - Amersham Hybond N problem C.L.L.
pHOOK (CaptureTec system) Nigel Kenward
mouse Raf genomic clones and sequence wanted Gregory Buzard
Abbreviated miniprep protocol... Incredible! Alexander Kraev
HELP! looking for N-ras intronic sequence. Gregory Buzard
Aedes graciela panzetta
***Multiplex PCR Hell****** negar moshiri
- ***Multiplex PCR Hell****** Dr G R Taylor
5' RACE Peta C. Bonham-Smith
- 5' RACE Steve Scherer
- 5' RACE vilimf01 at mcrcr6.med.nyu.edu
PCR probes by incorporation of 32P ? Geoffrey Neale
- PCR probes by incorporation of 32P ? Robert Horton
- PCR probes by incorporation of 32P ? Joseph C. Bagshaw
Anyone successfully doing RNAse protection Assays? Helmut
Magnetic RNA isolation M.W. SMITH
- Magnetic RNA isolation Reese Bolinger
  - Magnetic RNA isolation CPGYIELD
    - Magnetic RNA isolation: do-it-yourself beads Anton Scott Goustin
    - Magnetic RNA isolation: do-it-yourself beads vilimf01 at mcrcr6.med.nyu.edu
    - Magnetic RNA isolation: do-it-yourself beads Mark McCormick
inducible eukaryotic expression Robert Slany
Request for RELIABLE ssDNA rescue protocol for sequencin liz_caetta at stratagene.com
Adenovirus expression systems Valeri Krougliak
Alkaline Phosphatase substrates martin LEACH
Can one gene be used to select for OR against.... Paul N Hengen
5' RACE Mayumi Yagi
freeze-squeeze Paul N Hengen
Microplate technology Dr._Jose_Romero_at_WRAIR3__WASHDC at WRSMTP-CCMAIL.ARMY.MIL
Ribonuclease Protection Assay (RPA) Osama
- Ribonuclease Protection Assay (RPA) Steve Scherer
Sfi 1 enzyme tera
- Sfi 1 enzyme Jeff 'Newt' Sekelsky
Problems with Qiagen Maxipreps? helix.nih.gov
Alkaline Phosphatase substrates Pierce Chemical Co
used equipment Michael Bausher
- used equipment Jon Stewart
Sequencing after G run Michael Gorry
- Sequencing after G run Paul N Hengen
ELISA reagent supplier help needed! jchristn at umich.edu
- ELISA reagent supplier help needed! Keld Sorensen
- ELISA reagent supplier help needed! Tehcnical Assistance / Pierce Chemical Co
  - ELISA reagent supplier help needed! Dr.G.R.Rajasekariah
  - ELISA reagent supplier help needed! Dr.G.R.Rajasekariah
Help! Contract protein sequencing and phage display jchristn at umich.edu
Does anyone have an antibody to HSVtk? Phil Potter
purification of 140 Kbp from agarose Riekeltje Koedood
freezer purchase Riekeltje Koedood
rat cardiomyocyte cell line, who has one? Daniel
Making His-tag affinity resin Rita Lim
resending CBN info "Thro, Ann Marie ", 3701, by way of Prakash at acd.tusk.edu C. S. Prakash
alpha-S dNTPs Michael Lichten
inhibition of NF-kappa B at rzmail.uni-erlangen.de
DH5 alpha chip peele
- DH5 alpha David S. Lee
Abbreviated miniprep protocol... Incredible! pgegen at rnaworld.bio.ukans.edu
E. coli sec mutants Elliott Dawson
Ps fluorescens biovars? Don Chen
Background in Southern Blots Jerry Litt
- Background in Southern Blots Keld Sorensen
  - Background in Southern Blots Harry J. Witchel
BioRad GeneLite Kit Amie Franklin
Mitochondrial DNA extraction? Anita Gould
In situ hybridization Gabriel Cheung
SfiI enzyme Andrei Popov
test scnomura at gan2.ncc.go.jp
purification of 140 kbp from agarose Andrei Popov
Dye and terminator chemistry Microplate at eworld.com
Lignin Enzymes wanted tigney at compusmart.ab.ca
- Lignin Enzymes wanted Amie Franklin
  - Lignin Enzymes wanted Mick Partis
- Lignin Enzymes wanted Michael Bausher
- Lignin Enzymes wanted Paul E. Ulanch
hydrogenation Equipment Needed tigney at compusmart.ab.ca
recombinant thrombin jmorrey
SYBR Green staining of PAGE/urea gels bmay
Northern controls John Morlan
New life for old PCR templates? bmay
Anyone successfully doing RNAse protection Assays? vilimf01 at mcrcr6.med.nyu.edu
- Anyone successfully doing RNAse protection Assays? Tracy Aquilla
Biological Data Transport info R. Scott Jokerst
Anyone have indepth info on annealing temps and unzipping temp or equation for PCR components? JCM
- Anyone have indepth info on annealing temps and unzipping temp or equation for PCR components? Eitan Rubin
- Anyone have indepth info on annealing temps and unzipping temp or equation for PCR components? Eitan Rubin
Biotinylated Primer for S1 probe? phillip buckhaults
RNA isolation DANIEL Y KIM
purification of a 140 kb fragment Andrei Popov
EtOH + PBS = precipitation? Jim Hutchins
Superbroth for EVERYONE! (was NOT, but now IS) Robert Randolph
- Superbroth for EVERYONE! (was NOT, but now IS) Alex Merz
C127I cells & 6-TG Ashraf R. Dallol
Thymidine kinase antibody Ashraf R. Dallol
- Thymidine kinase antibody Hemayet Ullah
Anyone successfully doing RNAse protection Assays? Jim Voeller
- Anyone successfully doing RNAse protection Assays? CAShug
freeze-squeeze Michael Gillings
- freeze-squeeze Nigel Kenward
mitochondrial DNA database Jim Cummins
PCR Primers Database - UPDATE. Jim Cummins
Daudi-Permanent cell line kim myung jong
RNA isolation jms93
Plasmid Drawing Programme Tom Dougherty
Microplate technology Microplate at eworld.com
Aminopropanol mutant Jean-Francois Mayaux
Microinjection: where to find info about? Stefan Kahlert
Transfection of proteins into cells: HOW? Stefan Kahlert
- Transfection of proteins into cells: HOW? Brian T. Greuel
- Transfection of proteins into cells: HOW? elizabeth Ferguson
  - Transfection of proteins into cells: HOW? Stefan Kahlert
    - Transfection of proteins into cells: HOW? Dima Klenchin
    - Transfection of proteins into cells: HOW? Nigel Kenward
Polymerases, anyone!?!?!? Brett Beitzel
- Polymerases, anyone!?!?!? Martin_K_Burnham at SBPHRD.COM
Peptides Darren Boehning
Unltracompentent cells Lee Vogel
Plasmid Drawing Programs - where to find... R. Scott Jokerst
Cutting PCR-created restriction sites futers at biovax.leeds.ac.uk
Sequence of Lambda DASH II polylinker Anton Scott Goustin
Alpha Satellite Probes for Mice??? Where can you get them? FOR FISH Graham Dellaire
Protein gel Michael J. Tuvim
fluorescently labelled protein Keld Sorensen
fluorescently labelled protein davesmith at bioch.tamu.edu
- fluorescently labelled protein Computer
- fluorescently labelled protein Richard R. Hardy
Anyone have pcDNA II? mjb1 at Lehigh.EDU
Plasmid Drawing Programme Soren K. Rasmussen
Unspecific hybridization to plasmid-vector Manuel G. CLAROS
- Unspecific hybridization to plasmid-vector Ian A. Boussy
COS-cells David L. Haviland, Ph.D.
- COS-cells Mike
- COS-cells Allison Haggarty
  - COS-cells Christian Moews
- COS-cells Riekeltje Koedood
large-scale prep of ssDNA Yuandan Lee
- large-scale prep of ssDNA Duke Groebe
  - large-scale prep of ssDNA Michael Benedik
    - large-scale prep of ssDNA Kiley R. Prilliman
Transcription factors and transfections Karen Kroeger
Transfection of proteins into cells: HOW? brett
electroporation of Streptomyces sp. Kelly J. Thibault
- electroporation of Streptomyces sp. Dima Klenchin
Can dessicants be dried in microwave? Donald Chun Kit Wong
- Can dessicants be dried in microwave? David Joyce
- Can dessicants be dried in microwave? Bernard Murray
- Can dessicants be dried in microwave? Tommy the Terrorist
Can dessicants be dried in microwave? Michelle.Gleeson at SMTPGWY.AGRIC.NSW.GOV.AU
- Can dessicants be dried in microwave? Harry J. Witchel
- Can dessicants be dried in microwave? Mark Dowton
  - Can dessicants be dried in microwave? Nathan Siemers
Can dessicants be dried in microwave? Michelle.Gleeson at SMTPGWY.AGRIC.NSW.GOV.AU
- Can dessicants be dried in microwave? Mark Hill
Chemiluminescence, western blotting, problems... phillip buckhaults
anti-myristoylated glycine Ab Atsushi Ishikawa
freeze-squeeze Eitan Rubin
Why use 3´to5´exo-negative DNA-Polymerases? Horst Ibelgaufts
inducible eukaryotic expression Mike
rat cardiomyocyte cell line Frank Schaap
Native PAGE @ pH < 6.5 Christopher Walentas Bioc
Superbroth for EVERYONE! (NOT) elizabeth Ferguson
Hydropathy plot program Wayne Coco
Sources of baculovirus expression systems Tracy Aquilla
- Sources of baculovirus expression systems RMMMS at CUNYVM.CUNY.EDU
Differential centrifugation of cells and streptavidin-agarose? G.P.
- Differential centrifugation of cells and streptavidin-agarose? Mary Meza
PMSF timing Marc Visconti
(no subject) T. James
GAPDH mRNA half-life Peter G Bradford
TRI-REAGENT chip peele
Peptide SLIGRL schille at uni-muenster.de
- Peptide SLIGRL Ferland Louis H.
Membrane properties Michail P. Sahsamanoglou
- Membrane properties kramerse at wmvx.lvs.dupont.com
- Membrane properties Achim Recktenwald
Chemiluminescence, western blotting, problems... Giampaolo Minetti
- Chemiluminescence, western blotting, problems... Bernard Murray
- Chemiluminescence, western blotting, problems... John K. Troyer, PhD.
- Chemiluminescence, western blotting, problems... Hedrick Lab
- Chemiluminescence, western blotting, problems... Giampaolo Minetti
- Chemiluminescence, western blotting, problems... Surgical Residence
Northern blot and NybondN+membrane Joël Baguet
- Northern blot and NybondN+membrane Gerald Downes
  - Northern blot and NybondN+membrane ruth e.w. walker
inducible eukaryotic expression Michail Belyavskyi
Can one gene be used to select for OR against.... Sanjay Krishnaswamy
PCR-SSCP ON ABI 373 WITH GENESCAN 672 Carol Stugard
Biotech NewsReport C. S. Prakash
Want: Simple sequencing gel reader Bob Litt
- Want: Simple sequencing gel reader Phil Davey
- Want: Simple sequencing gel reader Frances Hannan Zoology
- Want: Simple sequencing gel reader Andy Phillips
TRI-REAGENT chip peele
RNA isolation Susan Patrick
- RNA isolation Mayumi Yagi
Quick Protein Concentration Assays Lee Weber
Touchdown PCR protocoll? Olivier Gandrillon
Inoue Competent Cell procedure Richard Friedman
- Inoue Competent Cell procedure Christian Moews
- Inoue Competent Cell procedure Ned Mantei
TA cloning nightmare Amna Saeed
- TA cloning nightmare Catherine I. DiOrio
  - TA cloning nightmare Dr. Duncan Clark
  - TA cloning nightmare Bob Horton
  - TA cloning nightmare Margon Vandongen
- TA cloning nightmare Steve Scherer
- TA cloning nightmare Martin Suhr
juks Mart Speek
TA cloning nightmare Amie Franklin
Superbroth for EVERYONE! (NOT) Dima Klenchin
Help-no plasmid!!! Mark Griffiths
- Help-no plasmid!!! burklab at aecom.yu.edu
- Help-no plasmid!!! Tim Barnett
Terminal transferase buffer tjames
HELP!---Immunoaffinity column doesn't work Jan Schroeder
GFP fusion problems fred wouters
Inoue protocol with reducing agents. And speedprep David Martin
Qiaex problems Lynne M Donegan
- Qiaex problems Catherine I. DiOrio
Primer Design j.r.stephen
- Primer Design Ed Castro
  - Primer Design jms93
    - Primer Design sites Ed Castro
    - Primer Design Xiu Yu
  - Primer design (Oligo) Bill Burnett
    - Primer design (Oligo) Benny Shomer
Re- Touchdown PCR Protocol Brett Beitzel
Reagent Availabilty Michael Kane
activated T-cell library for 2-hyb?? Heidi Moss
rare cutters 10-12 bp exist? Nancy Terryn
- rare cutters 10-12 bp exist? Gary K.
  - rare cutters 10-12 bp exist? Dr. Duncan Clark
Homemade shaking incubator Bob Horton
Representationel difference analysis ? bjorn eriksson
Prok. expression vectors; anything that is not based on Lac operon and Amp R? Hedrick Lab
- Prok. expression vectors; anything that is not based on Lac operon and Amp R? Mikhail Alexeyev
  - Prok. expression vectors; anything that is not based on Lac operon and Amp R? Hedrick Lab
    - Prok. expression vectors; anything that is not based on Lac operon and Amp R? Dr. Duncan Clark
    - Prok. expression vectors; anything that is not based on Lac operon and Amp R? Mikhail Alexeyev
    - Prok. expression vectors; anything that is not based on Lac operon and Amp R? Peter Wang
Northern controls John Morlan
(no subject) Rick Carroll
- removing RNA ruth e.w. walker
cDNA library kits-your choice Don Zuhn
- cDNA library kits-your choice RMMMS at CUNYVM.CUNY.EDU
  - cDNA library kits-your choice Andy Phillips
Hirt extracts Ian A. York
single-strand DNA break detection Scott Selby Med-Hem
RNA removal/quartz plate Rick Carroll
anti-HA cross reaction? Allison Haggarty
IMMUNOAFFINITY COLUMN DOESN´T WORK Tanja
Nicotiana spp. logan
ANNOUNCEMENT ingen at ingenlabs.com
PCR-SSCP ON ABI 373 WITH GENESCAN 672 Gregory Buzard
NEEDED: codon bias table for Pseudomonas Robert Johnston
- NEEDED: codon bias table for Pseudomonas Peter Wang
- NEEDED: codon bias table for Pseudomonas Hemayet Ullah
- NEEDED: codon bias table for Pseudomonas Shamima Akhter
ISO Plasmid Not1 & Mlu1 Sites DNAGuy
- ISO Plasmid Not1 & Mlu1 Sites Mikhail Alexeyev
anti-HA cross reaction? Ian A. York
Primer Extension Sequencing Steven Bressler
PCR Protocol for Mycoplasma Detection??? Carolyn Calnan
Prok. expression vectors; anything that is not based on Lac operon and Amp R? Edgar Valencia
Amersham Hybond N problems Michelle.Gleeson at SMTPGWY.AGRIC.NSW.GOV.AU
Robot Cycler Pascal_Bochet
- Robot Cycler Troels Wind
- Robot Cycler Marc Visconti
- Robot Cycler Kiley R. Prilliman
- Robot Cycler Ted Michelini
resolution on SDS-PAGE From Jaume Canaves
Is B-Gal phosphorylated in mammalian cells? Steven Enkemann
Anti-microbials Paul
Heat lability of DEPC Adrian.Philbey at smtpgwy.agric.nsw.gov.au
- Heat lability of DEPC P. Mertens
  - Heat lability of DEPC Alexander Kraev
  - Heat lability of DEPC Tracy Aquilla
    - Heat lability of DEPC simon
Simple sequencing gel readerS WSchick at aol.com
Very pure total RNA free of DNA? Agnes S. Chan
- Very pure total RNA free of DNA? Amie Franklin
- Very pure total RNA free of DNA? Simon Dawson
- Very pure total RNA free of DNA? ruth e.w. walker
- Very pure total RNA free of DNA? Michael Gillings
Announce WWW: Analytical Ultracentrifugation/XL-A Borries Demeler
Alamar inquiry bolivs at ene.engg.upd.edu.ph
Expression of GST fusion Gary T. Chou
- Expression of GST fusion Bernard Heymann
- Expression of GST fusion John Elliott
- Expression of GST fusion L. Fliegel
  - Expression of GST fusion Petri Kursula
electroporation Marcel Hoffmann
- electroporation yxu
low recovery with PromegaPolyATtract kit ? Toshiharu Ishizuka
- low recovery with PromegaPolyATtract kit ? Marc Lamphier
Opinion poll: Clonetech Matchmaker libraries Lee Vogel
- Opinion poll: Clonetech Matchmaker libraries Simon Dawson
  - Opinion poll: Clonetech Matchmaker libraries Jil Tardiff
Zonal gradients Juergen Brosius
- Zonal gradients Dr E. Buxbaum
Heat lability of DEPC Paul
rodent DNA and/or brains Juergen Brosius
Casein antibodies Gustavo Nolasco
- Casein antibodies Simon Dawson
Meeting - H.pylori Ronan O'Kennedy
low recovery with PromegaPolyATtract kit ? Catherine I. DiOrio
- low recovery with PromegaPolyATtract kit ? CPGYIELD
Taq stability? Susan Jane Hogarth
- Taq stability? Dr. Duncan Clark
  - Taq stability? Dubear Kroening
- Taq stability? Bob Horton
- Taq stability? Christoph Ahlers
  - Taq stability? Nicola Collie
Cycle Sequencing Robmit
- Cycle Sequencing Joseph C. Bagshaw
- Cycle Sequencing Alexander Kraev
Northern controls John Morlan
RT-PCR and cDNA synthesis from bacterial total RNA Oliver Politz
influenza hemagglutinin abs Mauro
- influenza hemagglutinin abs Thomas R. Anderson
  - influenza hemagglutinin abs Chris Boyd
Wizard maxipreps Surgical Residence
- Wizard maxipreps o0c9869 at tam2000.tamu.edu
DNA Isolation Kit Surgical Residence
Isolation of RNA chip peele
Leaky Mini-ProteanII unit. John J Weiland
- Leaky Mini-ProteanII unit. Dima Klenchin
- Leaky Mini-ProteanII unit. mdpjr at cc.newcastle.edu.au
  - Leaky Mini-ProteanII unit. Giampaolo Minetti
    - Leaky Mini-ProteanII unit. mdpjr at cc.newcastle.edu.au
  - Leaky Mini-ProteanII unit. Riekeltje Koedood
    - Leaky Mini-ProteanII unit. George Rutherford
    - Leaky Mini-ProteanII unit. Margon Vandongen
Spectrophotometric estimation of RNA SRINIVAS MUMMIDI
- Spectrophotometric estimation of RNA Anton Scott Goustin
ANNOUNCE: tacg - Restriction Enzyme analysis tool for unix Harry Mangalam
vectors poly (A) Jo=?iso-8859-1?Q?=EBl BAGUET
inducible eukaryotic expression ege
Leaky Mini-ProteanII unit. Paul N Hengen
Leaky Mini-ProteanII unit. HIRANYA ROYCHOWDHURY
Self competition in EMSA Menglin Luo
receptor cloning in mammalial cells Uwe
NH4Ac/EtOH ppt of DNA from PCR ? Royal Botanic Gardens
- any suggestion for electron autoradiography system b726711 at mailserv.cuhk.hk
- NH4Ac/EtOH ppt of DNA from PCR ? Dr. Duncan Clark
- NH4Ac/EtOH ppt of DNA from PCR ? DSeegert
optimize mammalial cloning Uwe
Store DNA concentrated or dilute? Susan Jane Hogarth
- Store DNA concentrated or dilute? Duke Groebe
  - Store DNA concentrated or dilute? Duke Groebe
  - Store DNA concentrated or dilute? Margon Vandongen
  - Store DNA concentrated or dilute? Bernard Murray
    - Store DNA concentrated or dilute? Margon Vandongen
    - Store DNA concentrated or dilute? Bernard Murray
    - Store DNA concentrated or dilute? Paul N Hengen
    - Store DNA concentrated or dilute? Stephen R. Lasky
- Store DNA concentrated or dilute? Richard Daines
Trace Acetylation Protocol From Jaume Canaves
Linking an oligonucleotide to an antibody Anton Scott Goustin
baculovirus co-infection BIOTEC54
Immuno(histo)fluorescence: what's the best method? Tommy the Terrorist
- Immuno(histo)fluorescence: what's the best method? Dr E. Buxbaum
Alamar inquiry WSchick at aol.com
Protein/carbohydrate chemist wanted simon
Polyacrylamide gel fixation for DNA Danny Leung
- Polyacrylamide gel fixation for DNA David R. Johnson
A fastidious contaminant Giorgio Spagnol
what is a cytofectin? Horst Ibelgaufts
Sequencing Gel Rigs for SSCP Dr G R Taylor
Looking for supplier pubh021
- Looking for supplier Andy Phillips
Clone fingerprinting Mr. A.R.J. Curtis
superbroth protocol finally finished Guivarc_h Dominic
(none) WSchick at aol.com
Clone fingerprinting Mr. A.R.J. Curtis
removal of starch (fwd) Baozhu Guo
Tungsten particles for biolistic techniques? klas at kvl.dk
CIBA-GEIGY phone#? bio_hannan at emuvax.emich.edu
RT-PCR Burkhard Hassel
Thymidine Kinase Hemayet Ullah
RT efficiency Michael HERMAN
(no subject) Bertrand COLLET
Protocol for Re-using of Qiagen tips Trevor Bezdek
- Protocol for Re-using of Qiagen tips Iain Fenton
Clone Fingerprinting --SSCP Gregory Buzard
Polyacrylamide gel fixation for DNA Gregory Buzard
ISO Plasmid with Not1 & Mlu1 Site DNAGuy
- ISO Plasmid with Not1 & Mlu1 Site Gary K.
Nuclease P1, Penicillium citrinum ProZyme
need Serva contact info Gary K.
Streptavidin in purification by affinity Dr._Jose_Romero_at_WRAIR3__WASHDC at WRSMTP-CCMAIL.ARMY.MIL
PCR through GC-rich MAN!!!
- PCR through GC-rich Dr. Duncan Clark
- PCR through GC-rich Koen De Smet
  - PCR through GC-rich Dale Levitzke
  - PCR through GC-rich Dale Levitzke
- PCR through GC-rich sreesh
- PCR through GC-rich Holger Steinlechner
Polyacrylamide gel fixation for DNA DNAGuy
need Serva contact info Dima Klenchin
Wizard preps ruth e.w. walker
REPOST Homemade Shaking Incubator Bob Horton
Transfection of proteins: HOW? Nikolai Chitaev
<a href="http://www.bio.net/hypermail/sponsor.html"><img src="http://www.bio.net/hypermail/sponsor.gif"></a><br>bionet.molbio.methds-reagnts archives: HELP!---Immunoaffinity column doesn't work Nikolai Chitaev
Wizard PCR purification from agarose gel ruth e.w. walker
- Wizard PCR purification from agarose gel Markku.M Jeltsch
- Wizard PCR purification from agarose gel A. John Watson
  - Wizard PCR purification from agarose gel Don Rollins
- Wizard PCR purification from agarose gel Tae Gwon Oh
  - Wizard PCR purification from agarose gel Colin Rasmussen
- Wizard PCR purification from agarose gel CLAREN321
Help: Rapid determination of copy No. of a gene in bacterial chromosome. Xiang Li
- Help: Rapid determination of copy No. of a gene in bacterial chromosome. gwade at physiology.uwo.ca
(Q) Problem w/silver enhancement of gold particles Kenneth J McNeil
Best chemiluminescence for W.blotting MALINI at JASON.UTHCT.EDU
- Best chemiluminescence for W.blotting bbraun at ucla.edu
  - Best chemiluminescence for W.blotting tera
    - Best chemiluminescence for W.blotting Jen Logan
    - Best chemiluminescence for W.blotting Keld Sorensen
    - Best chemiluminescence for W.blotting Keld Sorensen
    - Best chemiluminescence for W.blotting Bernard Murray
FPLC from Perseptive Biosystems? XZHENG at GAMMA.IS.TCU.EDU
Western blot mystery: update Pamela Norton
elisa plates??? LMordo
Clone fingerprinting Andy Phillips
Allelic Ladder-source needed? Garry P. Larson
Catalytically active purified Ras? Andrew Lange
Light Sensitivity of Tris Ferland Louis H.
Sequencing Gel Rigs for SSCP WSchick at aol.com
- Sequencing Gel Rigs for SSCP Bernhard
  - Addendum: Sequencing Gel Rigs for SSCP Bernhard Mayr
  - Addendum: Sequencing Gel Rigs for SSCP Bernhard Mayr
NdeI: cutting problems?? anyone else?? seems silly! Heidi Moss
- NdeI: cutting problems?? anyone else?? seems silly! Dr. Duncan Clark
  - NdeI: cutting problems?? anyone else?? seems silly! jovermeyer at shrsys.hslc.org
ATTO supplier JoanBoyce at aol.com
A fastidious contaminant John Ladasky
Distinct bands in restriction dig. of genomic DNA Markku.M Jeltsch
- Distinct bands in restriction dig. of genomic DNA Ned Mantei
- Distinct bands in restriction dig. of genomic DNA ae at dna.bio.warwick.ac.uk
  - Distinct bands in restriction dig. of genomic DNA Eitan Rubin
- Distinct bands in restriction dig. of genomic DNA Graham Dellaire
Gel Analysis - DNA ProScan David Ley
PCR method Andreas Ebrecht
RXR antibodies Eddie Diehl
- RXR antibodies mike Watson
RNAzolB...Still Available? Eric C. Anderson
How stable biotin-avidin? Marc Lamphier
- How stable biotin-avidin? Zophonias O. Jonsson
  - How stable biotin-avidin? Tehcnical Assistance / Pierce Chemical Co
  - How stable biotin-avidin? Paul N Hengen
  - How stable biotin-avidin? Michael Giffin
    - How stable biotin-avidin? CPGYIELD
  - How stable biotin-avidin? Dima Klenchin
    - How stable biotin-avidin? Mikhail Alexeyev
    - How stable biotin-avidin? Paul N Hengen
Protocol for RT-PCR in shrimp Sittiruk Roytrakul - SCBC - 3536459
- Protocol for RT-PCR in shrimp jms93
  - Protocol for RT-PCR in shrimp Tim F. Carl
Help? Immunoprecipitation Marc Lamphier
Degenerate primers for PCR HelenCS
- Degenerate primers for PCR Ted Michelini
- Degenerate primers for PCR Mr. Chris Hoyle
Polyacrylamide gel fixation for DNA Gregory Buzard
HRPO linking to oligos? Chris Kafer
Boomerang DNA Amplification Web Page Kevin Ahern
- Boomerang DNA Amplification Web Page Paul N Hengen
PALA only from NCI? Andreas Schaffer
Polyacrylamide gel fixation for DNA Gregory Buzard
The KlenTaq mystery Amna Saeed
- The KlenTaq mystery Dr. Duncan Clark
- The KlenTaq mystery Ted Michelini
Rhodobacter disruption Craig Turner
suitable loading controls for RPA's in diabetic vs. normal rats Nick Kallincos
- suitable loading controls for RPA's in diabetic vs. normal rats Steve Scherer
How to set up ELISAs Yasuhito Abe
Purifying clean plasmid vectors for ligation Harry J. Witchel
- Purifying clean plasmid vectors for ligation Mikhail Alexeyev
  - Purifying clean plasmid vectors for ligation Harry J. Witchel
- Purifying clean plasmid vectors for ligation Steven Goldberg
  - Purifying clean plasmid vectors for ligation Harry J. Witchel
    - Purifying clean plasmid vectors for ligation Dr. Duncan Clark
    - Purifying clean plasmid vectors for ligation Steven Goldberg
- Purifying clean plasmid vectors for ligation Amie Franklin
Hefe-Klonierung Georg Kroeger
Northern hybridizations PRETORIUS
- Northern hybridizations Stephen R. Lasky
- Northern hybridizations bbraun at ucla.edu
ANNOUNCE: NAOMI Upgrade Simon M. Brocklehurst
SDS PAGE buffers cost saving WSchick at aol.com
- SDS PAGE buffers cost saving mdpjr at cc.newcastle.edu.au
NdeI: cutting problems?? anyone else?? seems silly! (fwd) Neil Harris
- NdeI: cutting problems?? anyone else?? seems silly! (fwd) Gary Kucera
protoplast isolation JWU at LAN.TJHSST.EDU
Salmon sperm DNA preparation Rachelle Leger
- Salmon sperm DNA preparation CAShug
- Salmon sperm DNA preparation Karl Fischer
Large scale low melt extraction Mike Holbrook
- Large scale low melt extraction Paul N Hengen
- Large scale low melt extraction Karl Fischer
- Large scale low melt extraction K.James
  - Large scale low melt extraction J. Pat Martinez
- Large scale low melt extraction Alexander Kraev
cell line vdilbeck at dbm.ulb.ac.be
Inverse PCR Helen J. Hathaway, Ph.D.
- Inverse PCR vilimf01 at mcrcr6.med.nyu.edu
- Inverse PCR jovermeyer at shrsys.hslc.org
SEAP Unregistered User
transformation problems GILLIAN NI BHARLO BOTANY PG
- transformation problems CAShug
- transformation problems P. Mertens
- transformation problems Dr. Paul N. Brothers
Salmon sperm DNA preparation Alexander Kraev
- Salmon sperm DNA preparation Riekeltje Koedood
Carb vs Amp - whats the story? John Dixon
thrombin source for fusion prot cleav. GLUXLAB
Carb vs Amp - whats the story? Frances Hannan Zoology
SEAP ole morten Seternes
actinomycin D William Sun
western problem kim myung jong
tips for blunt ended ligation! Kenneth Y. Ilio
1500+ scientists obtain free BCMD subscriptions - bcmd.msg [1/1] Ernest Beutler, M.D.
Help needed with GST fusion protein. (pGEX vector) CBE Graduate Student
fax of SCHOTT in Germany or in France Pierre Commercon
1500+ scientists obtain free BCMD subscriptions - bcmd.msg [1/1] Ernest Beutler, M.D.
SEAP Bernard Murray
Carb vs Amp - whats the story? Paul N Hengen
tips for blunt ended ligation! Dilip Dias
tips for blunt ended ligation! Dilip Dias
tips for blunt ended ligation! Mikhail Alexeyev
- tips for blunt ended ligation! Eddie Diehl
  - tips for blunt ended ligation! Chris Boyd
PtdIns(3,4,5)P3 Where do I get it? George W Chacko
GeneClean II NaI conc??? Walter Hill
- GeneClean II NaI conc??? Gary K.
  - GeneClean II NaI conc??? Chip Peele
SDS PAGE buffers cost saving Phillip Robinson
elisa plates??? Microplate at eworld.com
GeneClean II NaI conc??? Shelley Cole
Help! My RNA has solidified!! wapling at staff.monash.edu.au
REAP Bertrand COLLET
Tn5-GM Neil Crickmore Bioc
Internalization of the protein into cultured cells Seong-Hun Kim
Fragment prep. using QiaTip? bjorn eriksson
(none) Hajime Sawada
35S-RFLPs Javier Quinteiro
Artifact in plasmid preps Dr. Duncan Clark
- Artifact in plasmid preps Mark R. Miglarese
- Artifact in plasmid preps Paul N Hengen
TA cloning GILLIAN NI BHARLO BOTANY PG
- TA cloning Jose M. Bautista
Protocol for Re-using of Qiagen tips Mads Noerregaard-Madsen
- Protocol for Re-using of Qiagen tips Harry J. Witchel
  - Protocol for Re-using of Qiagen tips Ferland Louis H.
    - Protocol for Re-using of Qiagen tips QIAGEN
    - Protocol for Re-using of Qiagen tips Ferland Louis H.
    - Protocol for Re-using of Qiagen tips Harry J. Witchel
- Protocol for Re-using of Qiagen tips Trevor Bezdek
VP16 antibodies Palle Serup
mRNA preps Rachelle Leger
Cloned DNA will not be cut hilde.nilsen at unigen.unit.no
test, please ignore Gary Kucera
Response to "Quick Protein Concentration Assays" BOYDSTON at MVAXGR.HUNTER.CUNY.EDU
human genomic library Markus Stefan Mueller
Testis library for two-Hybrid?? Michele Sallese
- Testis library for two-Hybrid?? Jon Nakamoto
NdeI: cutting problems?? anyone else?? seems silly! (fwd) logan
- NdeI: cutting problems?? anyone else?? seems silly! (fwd) Rich Kernan
- NdeI: cutting problems?? anyone else?? seems silly! (fwd) daniel morris
  - NdeI: cutting problems?? anyone else?? seems silly! (fwd) Bernard Murray
    - NdeI: cutting problems?? anyone else?? seems silly! (fwd) Arioch
Storage of serum in frost-free freezers? Eddie Diehl
- Storage of serum in frost-free freezers? Keld Sorensen
  - Storage of serum in frost-free freezers? Eddie Diehl
- Storage of serum in frost-free freezers? R. John Lye
RNA extraction-whole blood Neil Burns
- RNA extraction-whole blood Ian Graham
Tn5-GM Mikhail Alexeyev
Mercaptoethanol Shadows Frank Taddeo
- Mercaptoethanol Shadows Keld Sorensen
- Mercaptoethanol Shadows xzheng at GAMMA.IS.TCU.EDU
Glycoprotein Detection Neil Burns
- Glycoprotein Detection Keld Sorensen
- Glycoprotein Detection Iain Wilson
req. opinions of Ericomp thermocycler Brad Fabbri
- req. opinions of Ericomp thermocycler Don Rollins
  - req. opinions of Ericomp thermocycler lonnie
ANNOUNCE: Primers! for the Mac v1.0 Richard J. Resnick
Sfi1 enzyme tera
Sfi1 enzyme tera
Mercaptoethanol Shadows logan
elimination of cross reaction in polyclonal antiserum David P Alt
Agarose and preparative gels... HIRANYA ROYCHOWDHURY
- Agarose and preparative gels... Anthony Tomlinson
  - Agarose and preparative gels... HIRANYA ROYCHOWDHURY
Ribozymes Bradley A. Arrick
SDS-PAGE protein probs paul mc bride
Opinions on vaccuum centrifuge Andrea Crampton
RNA extraction from S. cerevisiae Tan Nguan Soon
Genescan and Diff Display Mona
POSITION AVAILABLE AT BIO-RAD LABORATORIES MacBoyee
Why serum starve before fos-jun induction ? Avirup Bose
- Why serum starve before fos-jun induction ? Mark R. Miglarese
Thymidine Kinase (Repost) Polash
PC12 cells Philippe Lefebvre
Why serum starve before fos-jun induction ? Adrian.Philbey at SMTPGWY.AGRIC.NSW.GOV.AU
mRNA preps Adrian.Philbey at SMTPGWY.AGRIC.NSW.GOV.AU
***ANYONE KNOW OF A GOOD FREEBIE PLASMID DRAWING PROGRAM?**** Dr Mark Watson
Guinea Pig B-actin seq. Help! Mr N.C. Eastmond
Pink fluorescein?? simon
SDS PAGE buffers cost saving logan
Peptide synthesis David Martin
- Peptide synthesis Iain Crowder
Methylen blue DNA staining joaroyo at samba.cnb.uam.es
- Methylen blue DNA staining Paul N Hengen
single band from rRNA from brachionus sp. paco
- single band from rRNA from brachionus sp. Joseph C. Bagshaw
Gibco's WWW site? G.P.
- Gibco's WWW site? Horst Ibelgaufts
  - Gibco's WWW site? Roger Baird
    - Gibco's WWW site? john brennand
    - Gibco's WWW site? martin LEACH
    - Gibco's WWW site? john brennand
    - Gibco's WWW site? Roger Baird
    - Gibco's WWW site? john brennand
- Gibco's WWW site? R. John Lye
- Gibco's WWW site? Annette
- Gibco's WWW site? john brennand
- Gibco's WWW site? Peter Hunziker
Pea genomic library paco
inosine primers Tm?? Mr D.P. O'Hare
- inosine primers Tm?? eric anderson
SpinBind Oliver S. DePeyer
SpinBind Oliver S. DePeyer
- SpinBind MAN!!!
Home-made reverse transcriptase. Richard Near
Re-post: PCR through GC-rich MAN!!!
- Re-post: PCR through GC-rich sreesh
- Re-post: PCR through GC-rich Dale Levitzke
Antisense gene knockout Richard Near
differential display Harm Deckers
Spectrophotometer Cuvettes Mr N.C. Eastmond
Spectrophotometer Cuvettes Mr N.C. Eastmond
A question about in situ hybridization Martin Downes
- A question about in situ hybridization Paul N Hengen
MICROSEQUENCING N-BLOCKED PROTEINS Andy Brauer
HELP : Stripping membranes with biotinylated proteins Patrice Boissy
SpinBind Oliver S. DePeyer
Bmh71-18 strain lperal
DNA precipitation? steve johnson
- DNA precipitation? daniel morris
Pyrimidine Dimers Brett Beitzel
Q: pRK5 vector sequence? AB
DNA precipitation? Gregory S. Buzard 'Buz'
Methods and Reagents FAQ list Paul N Hengen
direct blotting system LMordo
- direct blotting system Keld Sorensen
R.E.D. problems Andrew Hniat
Clontech Laboratories is a BIOSCI sponsor!! BIOSCI Administrator
Need help with Qiagen PB Buffer? Charles A Miller
Gibco WWW site Chuck Sohaskey
- Gibco WWW site Horst Ibelgaufts
RT of G-cap into C? vilimf01 at mcrcr6.med.nyu.edu
single band from rRNA from brachionus sp. (Dave Johnston)
UDP preparation shokhen
Shading Functional Domains Neil Harris
Internalization of the protein into cultured cells Yoo Hun Suh
cracked gels? Helen Korsmo
- cracked gels? Eddie Diehl
  - cracked gels? Bart Sefton
  - cracked gels? Riekeltje Koedood
- cracked gels? Darren Boehning
- cracked gels? Pascal_Bochet
- cracked gels? Dima Klenchin
- cracked gels? albano renee
  - cracked gels? ProZyme
Hsp70 Expression Vectors samueh01 at mcrcr6.med.nyu.edu
Does TK work in Neuro-2a cells? Matthew P. Frosch, M.D., Ph.D.
What size DNA stays in soln when NH4AC ppt? John Dixon
Isolation of nulcei Kristina Warton
- Isolation of nulcei Dr E. Buxbaum
  - Isolation of nulcei s1050589 at iplabs.ins.gu.edu.au
  - Isolation of nulcei s1050589 at iplabs.ins.gu.edu.au
sequencing murine p53 (fwd) Ashraf R. Dallol
cDNA-Library of monocytes melkony at wwupop.uni-muenster.de
Genetic Meeting Jena Johannes Woestemeyer
mRNA Differential Display Harm Deckers
Glutathione redox buffer? Csaba Jeney
anti 5-lipoxygenase antibodies fred wouters
- anti 5-lipoxygenase antibodies Dr. Stephen Hoare
pAX Fusion Protein Vectors Krish Sakhamuru
- pAX Fusion Protein Vectors Joao Vasconcelos Costa
- pAX Fusion Protein Vectors Torsten Boerchers
Protein inhibitor(s) during immunoprecipitation ? Dr. P.A. de Zoysa
- Protein inhibitor(s) during immunoprecipitation ? Mark R. Miglarese
  - Protein inhibitor(s) during immunoprecipitation ? Dr. Stephen Hoare
    - Protein inhibitor(s) during immunoprecipitation ? Bernard Murray
Storage of serum in frost-free freezers? Jfloring at aol.com
Lambda exonuclease Robin Beech
cracked gels? Curt Ashendel
- cracked gels? Dr E. Buxbaum
What size DNA stays in soln when NH4AC ppt? Riekeltje Koedood
Yolky DNA s060965 at AIX1.UOTTAWA.CA
- Yolky DNA vilimf01 at mcrcr6.med.nyu.edu
disrupting filimentous fungi Andrew Hopkins
- disrupting filimentous fungi Dima Klenchin
- disrupting filimentous fungi William Moens
Mutagenesis kits Rick Neubig
- Mutagenesis kits Elizabeth Rogers
Gibco Site Patrick HJ Falckh
RT of G-cap into C? Anton Scott Goustin
Transcription Factors Sergey V. Orlov
anti 5-lipoxygenase antibodies Sailesh Surapureddi
what is a Far South-Western blot? Horst Ibelgaufts
SpinBind Oliver S. DePeyer
- SpinBind A.S.
  - SpinBind and Supelco Oliver S. DePeyer
    - SpinBind and Supelco Jasper Saris
some information about hormones needed... Sergey V. Orlov
Ab to inositol monophosphatase Michael Kane
Bmh71-18 strain Mark R. Miglarese
Free Sulphydryl detection Michael Daws
- Free Sulphydryl detection Keld Sorensen
- Free Sulphydryl detection Achim Recktenwald
  - Free Sulphydryl detection "Marianne Leverone ", BIO
    - Free Sulphydryl detection David Martin
Flow Cytometer Surasakdi Wongratanacheewin
mouse lymphotoxin David Knight
FloriGen newsgroup keyword search Bill McKendree
PCR: How to remove DNA contamination from the starting mRNA? Simon Golding
20% gel drying Laigle
- 20% gel drying Kaj Stenberg
Plasmid Drawing Program MARK PEGGIE
PCR from saliva? Igor R Sagdeev
Light Sensitivity of Tris Per Bergman
Bmh71-18 strain Michael Benedik
PCR contamination-HELP! Warren D. Kruger
- PCR contamination-HELP! Harry J. Witchel
- PCR contamination-HELP! vilimf01 at mcrcr6.med.nyu.edu
- PCR contamination-HELP! David Hosfield
- PCR contamination-HELP! Riekeltje Koedood
  - PCR contamination-HELP! Morrison Lab
fidelity of reverse transcriptase Regina Shaw
in-situ hyb in lung: method of fixation? Gil
Help: Lambda purification kits Ulrich Harttig
- Help: Lambda purification kits john brennand
FBS users PLEASE READ - IMPORTANT martin LEACH
- FBS users PLEASE READ - IMPORTANT Keld Sorensen
- FBS users PLEASE READ - IMPORTANT martin LEACH
PCR: How to remove DNA contamination from the starting mRNA? Mark R. Miglarese
PCR amplification of mouse kappa chains H. David Fischer
Bmh71-18 strain Karl Fischer
RNA isolation/Chomozynski Susan Patrick
- RNA isolation/Chomozynski Ferland Louis H.
RNA isolation/Chomozynski Susan Patrick
- RNA isolation/Chomozynski Daniel Pereira
RNA isolation/Chomozynski Susan Patrick
- RNA isolation/Chomozynski Patrick HJ Falckh
methylation of DNA in mice mrtour at stud.med.cornell.edu
longggg mRNA transcript benan dincturk
- longggg mRNA transcript Konstantin M. Chumakov
  - longggg mRNA transcript Bob Horton
Spinach library benan dincturk
Reuse of Qiagen Ni-NTA Spin columns?? Bradley Turner
PCR HELP! DXS52, Xq28 (St14 VNTR) anybody with experience? John Wolff
- PCR HELP! DXS52, Xq28 (St14 VNTR) anybody with experience? Dr G R Taylor
Lipase assay ZOOMmmm
- Lipase assay "Marianne Leverone ", BIO
2D-Software Harry Reis
- 2D-Software eqpcmv at univ-lille1.fr
- 2D-Software eqpcmv at univ-lille1.fr
Northern hybridizations logan
PCR AMPLIFICATION OF MOUSE KAPPA CHAINS Andrei Popov
phage display using Stratagene's Surfzap Kit Alex Chang
- phage display using Stratagene's Surfzap Kit tera
- phage display using Stratagene's Surfzap Kit Alex Chang
  - phage display using Stratagene's Surfzap Kit tera
Circular Dichroism r.jackson at ic.ac.uk
SOS!--PCR restriction cut Wenjin Yu
- SOS!--PCR restriction cut Ashok Aiyar
  - SOS!--PCR restriction cut Kiley R. Prilliman
- SOS!--PCR restriction cut C.L.L.
- SOS!--PCR restriction cut ProZyme
  - SOS!--PCR restriction cut Bob Horton
seeking protocal for RNase assay by gel electrophoresis Peter
2D-Software WSchick at aol.com
Looking for UV-DNA endonuclease Michael Adamson
PCR from saliva? Adrian.Philbey at SMTPGWY.AGRIC.NSW.GOV.AU
enantiomeric amino acids Michael Moormann
Exogenous ATP supply - help requested Neil Winegarden
- Exogenous ATP supply - help requested john brennand
What is Touchdown PCR? spyong at pc.jaring.my
long mRNAs benan dincturk
RE- PCR Contamination Brett Beitzel
Pumps for Zonal Rotors Juergen Brosius
Antibodies against inclusion body proteins? Simon Dawson
PCR ampl. of a variable locus Juergen Brosius
SOS!--PCR restriction cut Edgar Valencia
- SOS!--PCR restriction cut Dr. Duncan Clark
What is Touchdown PCR? Craig Echt
Mycoplasma detection kits, and mycoplasma effects Ian A. York
PCR mutagenesis and proofreading polymerase prscholl
P.aeruginosa PAO-1 "Marianne Leverone ", BIO
PCR mutagenesis and proofreading polymerase Margon Vandongen
What is Touchdown PCR? Paul N Hengen
PCR stutters through oligo-A regions Juergen Brosius
- PCR stutters through oligo-A regions vilimf01 at mcrcr6.med.nyu.edu
  - PCR stutters through oligo-A regions Juergen Brosius
    - PCR stutters through oligo-A regions Pamela Norton
    - PCR stutters through oligo-A regions Juergen Brosius
Sequence-primer for lambda Pl? Troels Wind
Rainin edp plus Lesley Weston
- Rainin edp plus Michael Bausher
- Rainin edp plus batteries davesmith at bioch.tamu.edu
Source of Proteoglycans? Bradley Turner
Green Fluorescent Protein Kent Hill
- Green Fluorescent Protein Dr. Duncan Clark
- Green Fluorescent Protein L. Fliegel
how to incubate Ab/membrane? J Quyen Wickham
PCR stutters through oligo-A regions logan
Shrimp virus assay Joseph C. Bagshaw
Wizard PCR purification from agarose gel Song-Kun Shyue
PCR mutagenesis and proofreading polymerase upyers
PCR stutters through oligo-A regions Jeffrey D. Bray
Slow dye for sequencing gels? Stephen Morris
Gene clean reagent list. Ron Sayler
- Gene clean reagent list. Simon Dawson
A260 or Fluoromete ??? Which is correct Shaun Tyler
- A260 or Fluoromete ??? Which is correct john brennand
TNT kit with T7 RNA Pol HONG
- TNT kit with T7 RNA Pol Hans Bernd Prisack
  - TNT kit with T7 RNA Pol Margon Vandongen
  - TNT kit with T7 RNA Pol Kojo Mensa-Wilmot
- TNT kit with T7 RNA Pol Hans Bernd Prisack
  - TNT kit with T7 RNA Pol Jen Logan
    - TNT kit with T7 RNA Pol Jennifer Lyon
silver stain LAU Tze Chin
- silver stain Michael West
Green Fluorescent Protein Purification Short Courses Daniel Gonzalez
What is Touchdown PCR? NOT IDAHO WSchick at aol.com
Green Fluorescent Protein WSchick at aol.com
- Green Fluorescent Protein A.Doherty
- Green Fluorescent Protein A.Doherty
Pumps for Zonal Rotors WSchick at aol.com
Site directed mutagenesis kits -recommend? Sonya Clark
- Site directed mutagenesis kits -recommend? john brennand
- Site directed mutagenesis kits -recommend? Mark R. Miglarese
- Site directed mutagenesis kits -recommend? tera
- Site directed mutagenesis kits -recommend? at
  - Site directed mutagenesis kits -recommend? Margon Vandongen
- Site directed mutagenesis kits -recommend? Dr. Paul N. Brothers
- Site directed mutagenesis kits -recommend? Eckhard Boles
isolation of small DNA fragments CDMENU at ccs1.cc.monash.edu.au
- isolation of small DNA fragments Duke Groebe
  - isolation of small DNA fragments Jeff Smerage
- isolation of small DNA fragments Harry J. Witchel
PCR contamination Neil Burns
Qiagen RNeasy: Early precipitate?? Jim Hill
Any commercially available plasmids with HA, myc or T antigen tags. Obaid Y. Khan
Solaris (Unix) on IntelPC Don Gilbert
Blocked Xba I sites? Todd Miller - Pharmacology
transfection standard Soeren Jensby Nielsen
Transfection of lymphoid leukemia cell lines Richard Moldwin
SolarisX86 Bradley Turner
Acetylcholinesterase (AChE) regeneration after DFP? Alan
Anyone heard of pCMV7? KPH
- Anyone heard of pCMV7? Michael Daws
T4 ligase clone Scott Vande Pol
chickens vs rabbits for antibodies Scott Vande Pol
- buy GP120 peptide? Russ Boggs
- chickens vs rabbits for antibodies David Martin
- chickens vs rabbits for antibodies L. Fliegel
  - chickens vs rabbits for antibodies Richard Van Frank
- chickens vs rabbits for antibodies Robert Randolph
oligo annealing/cloning problem Robert F. Leclerc
- oligo annealing/cloning problem Daniel Pereira
Hsp70 Expression Vectors RMMMS at CUNYVM.CUNY.EDU
- Hsp70 Expression Vectors Seng-Lai Tan
messy agarose bands J.C.
- messy agarose bands John Elliott
  - messy agarose bands john brennand
? Re: Representational Difference Analysis Teresa Shippy
RNase protections assays. Too much salt? Angela Hofstra
- RNase protections assays. Too much salt? Pamela Norton
  - RNase protections assays. Too much salt? CAShug
- RNase protections assays. Too much salt? Daniel Pereira
  - RNase protections assays. Too much salt? Brian M. Shewchuk
PCR: How to remove DNA contamination from the starting mRNA? QIAGEN
Acetylcholinesterase (AChE) regeneration after DFP? Achim Treumann
- Acetylcholinesterase (AChE) regeneration after DFP? T. Chyau Liang
Expression vector tags Jon Hobman
- Expression vector tags RMMMS at CUNYVM.CUNY.EDU
- Expression vector tags John Elliott
- Expression vector tags John Elliott
  - Expression vector tags Kiley R. Prilliman
IFN-treated cell cDNA library Seng-Lai Tan
Touchdown PCR Joao Vasconcelos Costa
- Touchdown PCR Paul N Hengen
transcription factor binding sites software? Eckhard Boles
- transcription factor binding sites software? Bob Horton
A260 or Fluoromete ??? Which is correct WSchick at aol.com
PCR-cloning P. Mertens
A260 or Fluoromete ??? Which is correct john brennand
Media Question: What is Standard-1 from E. Merck? Paul N. Brothers
POSITION AVAILABLE: Research Asst. Grtful ned
Req: Electroporation of myloid and megakaryocyte cell lines, HELP?? Carmelo Volpe
RE- Touchdown PCR Brett Beitzel
ALF-express Toni Whistler
? differntial PCR jms5 at Lehigh.EDU
Teicoplanin antibiotic (where can I get it) Al
expression vector tags Tony Sanchez
A260 or Fluoromete ??? Which is correct Bradley Turner
Qiagen RNeasy: Early precipitate?? QIAGEN
Need help with Qiagen PB Buffer? QIAGEN
In vivo expression vectors Job Tharappel
Primer extention analysis by PCR Toshio
- Primer extention analysis by PCR Ken Howe
  - Primer extention analysis by PCR Paul N Hengen
- Primer extention analysis by PCR Russell L Wrobel
PCR on cells in suspension Tony Sanchez
Wanted: tk promoter - b gal construct Changcun Jia
- Wanted: tk promoter - b gal construct helix.nih.gov
ß-Amyloid peptide solubility Joaquin Arribas
Anybody working with VEGF? tick
Reply: DN Ligation Andrea Krempler
- Reply: DN Ligation Paul N Hengen
  - Reply: DN Ligation David Micklem
  - Reply: DN Ligation Pamela Norton
Colourimetric CAT assay? Oksana K. Yarosh
5' flourescein labelling and ABI, anyone tried it
How does pH effect Terrific Broth? Charles A Miller
Ethidium bromide stained acrylamide gels for microsatellites Rick Johns
- Ethidium bromide stained acrylamide gels for microsatellites Ned Mantei
small PCR product-->ligation luis
- small PCR product-->ligation Gary Kucera
How does pH effect Terrific Broth? Ted Michelini
- How does pH effect Terrific Broth? asdas
A260 or Fluoromete ??? References WSchick at aol.com
PCR in Nepal Dr. J.P. Clewley
- PCR in Nepal Bob Horton
Protein purification Inmaculada Garcia
Info about Auto Sequencer...! pramod at nii.ernet.in
- Info about Auto Sequencer...! Steven Goldberg
HIV p24 Antigen Administrador del nodo
HEX-labelled primer doesn't work in PCR ? Shirley Horn-Saban
Protein purification Jose M. Bautista
Inoue competent cell procedure Arfaan Rampersaud
- Inoue competent cell procedure Rico Laage
- Inoue competent cell procedure john brennand
  - Inoue competent cell procedure Paul N Hengen
Need to find source of teicoplanin Al
Need to find source of teicoplanin Al
Anyone heard of pCMV7? (fwd) Jan Ulrik Thorup
anti dsDNA latex agglutination Rafel Ferragut
TROPIX Dual-LIght P. Vijay Sarthy
Non-rad. Northerns Scott Selby Med-Hem
- Non-rad. Northerns Rico Laage
Kan resistance and pH Rafael Maldonado
Anyone using the ABI Catalyst 800 for genotyping? Don Bowden
ALPHA ANNOUNCES NEW PROCEDURES TO ENHANCE PLASMA PRODUCT SAFETY John Gross
Extra Long PCR reamplification Konstantin M. Chumakov
- Extra Long PCR reamplification upyers
Strep fusion protein vector? William_P_Prichett
HELP! GST column with Tris-Saline? sujoy ghosh
- HELP! GST column with Tris-Saline? Michael DiDonato
  - HELP! GST column with Tris-Saline? Cornelius Krasel
Taq Extender--degrading A overhangs! Jeffrey Shane Temple
- Taq Extender--degrading A overhangs! Margon Vandongen
- Taq Extender--degrading A overhangs! vilimf01 at mcrcr6.med.nyu.edu
- Taq Extender--degrading A overhangs! Holger Steinlechner
PCR contamination Shaun Tyler
- PCR contamination Osama
Modified pGEM? Angel Yanagihara
- Modified pGEM? S John
Any mass spec for protein sequencing under $30,000? XZHENG at GAMMA.IS.TCU.EDU
- Any mass spec for protein sequencing under $30,000? Eddie Diehl
  - Any mass spec for protein sequencing under $30,000? asdas
LI-COR, Inc. sponsors BIOSCI! BIOSCI Administrator
Where can obtain AMVN .... Depto. Biologia Celular
e-mail Piatak Administrador del nodo
RNA mass ladder greater than 10kb Martin Collinson
GST Fusion stuck on sepharose John
- GST Fusion stuck on sepharose Simon Dawson
- GST Fusion stuck on sepharose David Roof
Fog in In Vitro Translations John
- Fog in In Vitro Translations Lee Weber
- Fog in In Vitro Translations Arthur Wohlwill
- Fog in In Vitro Translations Michael Morales
pcDV1 sequence mpoulin at s-and-s.com
RNA mass ladder greater than 10kb Steven Tait
5'-> 3' Yegor Vassetzky
PCR can not get intense bands KHUANG at CHEMVX.TAMU.EDU
Fragment prep. using QiaTip? QIAGEN
yeast transformation efficiency Ray Lu
- yeast transformation efficiency S.L. Rennie BSc, Lab Manager
  - yeast transformation efficiency ZYMO RESEARCH
Inoue competent cell procedure Bradley Turner
Inoue competent cell procedure Bradley Turner
PCR can not get intense bands HIRANYA ROYCHOWDHURY
- PCR can not get intense bands Bernard Murray
Structural Biochemistry Jose M. Bautista
calyculin A Annick Breton
Custom Antibodies Gustavo Nolasco
Techniques to study genetic drift (query) Ravi Menon
USB t4 DNA ligase ----PROBLEMS? teb3 at Lehigh.EDU
Primer extension by rTth Reverse transcriptase ? Toshio
- Primer extension by rTth Reverse transcriptase ? phillip buckhaults
storage of E.coli Administrador del nodo
Transfection of HL60 Robert Slany
- Transfection of HL60 L. Bastian
magnetophoretic systems lonnie
PCR can not get intense bands Zahra Fayazi
PCR can not get intense bands Zahra Fayazi
- PCR can not get intense bands P. Mertens
- PCR can not get intense bands Dr G R Taylor
- PCR can not get intense bands Dr G R Taylor
Looking for a histochemical ATPase protocol... Jil Tardiff
- Looking for a histochemical ATPase protocol... Matthew P. Frosch, M.D., Ph.D.
- Looking for a histochemical ATPase protocol... H.Hoppeler
- Looking for a histochemical ATPase protocol... H.Hoppeler
Inoue Competent Cells Amna Saeed
The Nest Group, Inc. sponsors BIOSCI! BIOSCI Administrator
Homemade Shaking Incubator - decode pictures Robert Horton
(none) Administrador de iihema.fmed.uba.ar
(none) Administrador de iihema.fmed.uba.ar
Help: looking for a source for phytochelatin. Jin-Hong Kim
substractive libraries: suggestions ?? Jean-Yves Masson
Heterologous probe a problem in Southern spyong at pc.jaring.my
- Heterologous probe a problem in Southern Steven Goldberg
Seeking Proteoglycan Source Bradley Turner
Reply: DN Ligation UV Filter WSchick at aol.com
Antibody(ies) to Rat Aldolase(s)..........anybody? David Woodcock
What size DNA stays in soln when NH4AC ppt? Xiaohui Ji
IMPORTANT - BIOSCI Fundraising Update! BIOSCI Administrator
Boomerang, Vector-Splinker ette: Any success? Juergen Brosius
southern blotting of small fragments Liam E Good
Tyramide Signal Amplification mark throsby
- Tyramide Signal Amplification Jerry Litt
semi-blotting Liam E Good
Tyramide Signal Amplification Keld Sorensen
glycogen as a carrier Ari Kahn
- glycogen as a carrier Colin Rasmussen
  - glycogen as a carrier John Dixon
- Linear acrylamide (was --Re: glycogen as a carrier) Glen Shearer
  - Linear acrylamide (was --Re: glycogen as a carrier) john brennand
  - Linear acrylamide (was --Re: glycogen as a carrier) David Grant
- glycogen as a carrier Simon Dawson
Detection of molecular weight marker bands on +tively charged membranes labimap
- Detection of molecular weight marker bands on +tively charged membranes ProZyme
- Detection of molecular weight marker bands on +tively charged membranes Ed Stephenson
subscribe Don Faller
PCR Lyme Disease (DNA extraction protocol from serum) Paulo Santos
porin production in E. coli - advice needed Jean-Yves Paquet
- porin production in E. coli - advice needed Dilip Dias
- porin production in E. coli - advice needed Dilip Dias
- porin production in E. coli - advice needed Dilip Dias
Trial sending Osama
HELP : Dosage of biotinylated proteins Patrice Boissy
- concentration of biotinylated proteins Keld Sorensen
how to get rid of ethidium bromide??? Arioch
- how to get rid of ethidium bromide??? Brian M. Shewchuk
- how to get rid of ethidium bromide??? Bob Horton
- how to get rid of ethidium bromide??? Anthony Tomlinson
CNBr and phosphate groups Randall Willis
- CNBr and phosphate groups Bart Sefton
antibodies to tetM Laura Conway
Luminimetry with luciferase: wavelength please! I.R. Franklin
- Luminimetry with luciferase: wavelength please! Glenn A. Warr
Re- Isolation of small DNA Brett Beitzel
Re- PCR contamination Brett Beitzel
Alternative to autoclaving for denucleasing plasticware zeus
- Alternative to autoclaving for denucleasing plasticware Ned Mantei
DMSO and bacteriophage Helen Logan
- DMSO and bacteriophage john brennand
ANTI-RAT ANTIBODIES Chris Regan
Post-doc needed Jay L. Rothstein
- Post-doc needed Klaus Salger
POST DOC AVAILABLE gabre
storing gels... Arioch
- storing gels... Monica L. Parker
semi-dry elecrophoretic xfer Vs wet e.phoretic xfer ... Arioch
DNA binding Protein Inhibition Tina Hallis
- DNA binding Protein Inhibition Duke Groebe
- DNA binding Protein Inhibition Pamela Norton
Radiolabelled phosphoinositols Jon Church
German drug screening, assay, metabolism vendors? Philip W. Stephenson
Northern blots without formaldehyde Doug Johnson
- Northern blots without formaldehyde Amie Franklin
- Northern blots without formaldehyde Klaus Salger
- Northern blots without formaldehyde Glen Shearer
are introns neccessary for transgene expression Liz Mann
- are introns neccessary for transgene expression Michael Morales
Siliconized coverslips for In Situ Hybridization wgarrett at GGPL.ARSUSDA.GOV
- Siliconized coverslips for In Situ Hybridization Gabriele Kerber
- Siliconized coverslips for In Situ Hybridization ingen at ingenlabs.com
are introns neccessary for transgene expression milstone
Antibodies against dermatophytes Steffen Roth
Excision of LambdaZap Products Robert A. Schoenfeld
- Excision of LambdaZap Products Alexander Kraev
- Excision of LambdaZap Products Jon Stewart
Scintillation Counting Without Scintillation Fluid Philip P Calvert
- Scintillation Counting Without Scintillation Fluid john brennand
Help about blood storage please! Jimmy Rail
- Help about blood storage please! P. Mertens
- Help about blood storage please! Dave Hostler
- Help about blood storage please! Dave Hostler
- Help about blood storage please! mikem at s-and-s.com
Help: Ribonuclease protection assays. Married with Children
- Help: Ribonuclease protection assays. CAShug
Gibco's WWW site restriction on server? Microplate at eworld.com
Help : Hygromycin dose for Daudi kim myung jong
Alternative to autoclaving for denucleasing plasticware Microplate at eworld.com
SDS in western blot transfert Lionel Berthoux
- SDS in western blot transfert Dima Klenchin
- SDS in western blot transfert Michael Bausher
PCR-primer with T7 Promotor for TK Friedemann Weber
commercial adenovirus vectors bezstarosti at bc1.fgg.eur.nl
Gel documentation system K. Wernars
info on HPLC and related techniques Marc Visconti
- info on HPLC and related techniques John Elliott
p53's apoptotic function mrtour at stud.med.cornell.edu
- p53's apoptotic function Jean-Philip Truman
- p53's apoptotic function Bob Scibienski
- p53's apoptotic function CSJGH at leeds.ac.uk
Genetically Directed RDA? Teresa Shippy
Stripping Streptavidin-HRP Michael Daws
Stripping Streptavidin-HRP Michael Daws
Immunohistochemistry: Technique Question Auditorium
pZErO plasmid George Clark
- pZErO plasmid Richard Daines
- pZErO plasmid Arnoud Kal
Positive control for PTK probe Tracy E. Anderson
Help needed for DNase I Footprinting Dominic Voon
Automated sequencing reactions mikel at vaxc.cc.monash.edu.au
- Automated sequencing reactions phillip buckhaults
Immunohistochemistry: Technique Question Jerry Clayton
**Anyone have a murine Interferon gamma expressing plasmid or cassette??*** Dr Mark Watson
Help needed for DNase I Footprinting Ferland Louis H.
PCR in Nepal WSchick at aol.com
Hsp70 Expression Vectors Bradley Turner
info about NTCB Patrick Van
- info about NTCB Krishnaswamy
PC version of FASTA vipond at BSA.BRIS.AC.UK
List of Genome projects david johnston
Seeking "Bioconsumer Review" Bradley Turner
Vascular Endothelial and Smooth Muscle Cells John Fox
Antibodies required..badly! Ashraf R. Dallol
Transforming bacteria for protein production Patrick Grealish
- Transforming bacteria for protein production Vanhauwe Jurgen
Is there a FAQ for this group? Patrick Grealish
Has anyone done NF1 (The transcription factor) Northerns? EILEEN
Seeking "Bioconsumer Review" FREE WSchick at aol.com
Seeking "Bioconsumer Review" Keld Sorensen
Re- pZErO Brett Beitzel
- Re- pZErO Steven Goldberg
In situ hybridisation GILLIAN NI BHARLO BOTANY PG
Hoeffer Progenetor II electroporation unit Robert Coelen
Is there a FAQ for this group? Paul N Hengen
Thanks for tips for GC-rich PCR! MAN!!!
Differential Display PCRs - help needed! Miss J.M. Cameron
- Differential Display PCRs - help needed! Louis Alarcon
chylomicron retention Karl G. Kohlgraf
Repetitive Northern Hybridization Gabriel Romero
Mycoplasma detection kits, and mycoplasma effects Bradley Turner
- Mycoplasma detection kits, and mycoplasma effects A.Doherty
Getting cells off plates Pearse Ward
- Getting cells off plates Dima Klenchin
- Getting cells off plates simon
- Getting cells off plates L. Bastian
  - Getting cells off plates Mike Gruidl
Need to remove nucleic acids from nuclear extracts ##Sophie## ##Candon##
- Need to remove nucleic acids from nuclear extracts joestudent at mit.edu
free sequence homology search on WWW Jane O'Brien
- free sequence homology search on WWW Juergen Brosius
Getting cells off plates Edward Wang
- Getting cells off plates John Fox
- Getting cells off plates Tom Vink
  - Getting cells off plates Jen Logan
Phage DNA Isolation KHUANG at CHEMVX.TAMU.EDU
- Phage DNA Isolation john brennand
EtBr optimal emission spectra Torsten Karlsson
RNA isolation- the long and the short of it... przemko
Site-directed PCR mutagenesis Hans Kiefer
- Site-directed PCR mutagenesis Philippe Lefebvre
tuning of expression levels Marcel Hoffmann
source for ganciclovir Dima Klenchin
source for ganciclovir Lyn A. Thet
EcoRI adaptors Jane Q. Employee
Help primer extention analysis Toshio
SOS Call to all experinced "Sequencers" spyong at pc.jaring.my
- SOS Call to all experinced "Sequencers" Anthony Tomlinson
LCR (Ligase Chain Reaction) kit Abraham Rosenberg
Expand PCR and TA cloning kits? John Dixon
- Expand PCR and TA cloning kits? Margon Vandongen
RNA isolation- the long and the short of it... Shelley Cole
How to put a phosphate group on 3'OH of ssDNA? Robert SCHROCK
Zonal Rotor vibrations craig gatto
TOL Plasmid Prep - protocol K.G. Korth
SOS Call to all experinced "Sequencers" Michael DiDonato
Help with Transcription Factors Database? Jimmy Rail
- Help with Transcription Factors Database? username at bay.cc.kcl.ac.uk
Plant Expression Vectors Dr. Randal W. Giroux
Expand PCR and TA cloning kits? tera
rxn of protein a with denatured ab ProZyme
good sequencing unit christine-elsik at ansc.tamu.edu
- good sequencing unit Ted M.
Plant Expression Vectors Masashi Ugaki
Drying sequencing gels John Rebers
- Drying sequencing gels HIRANYA ROYCHOWDHURY
- Drying sequencing gels Anthony Tomlinson
Polymerase fidelity and KlenTaq suppliers Brian Shipley
What is "Cleavase" Raymond Dalgleish
GIBCO:Gene-trapper Kit Tegelk at ear002.uni-muenster.de
Pellet paint ? john brennand
Virtual Reality in SYBR Space Wayne Coco
DNA base composition Joseph C. Bagshaw
Peptide Nucleic Acids (PNA) Mick Jones
Anyone heard of this company? Marc Visconti
EcoRI adaptors john brennand
Cutting Qiagen miniprebs?? Bjoern Voldborg
- Cutting Qiagen miniprebs?? Richard Daines
A Budded Yeast: One or Two? Namjin Chung
EcoRI adaptors James R. Miller
Yeast Knockout? Namjin Chung
SYBR Reply STEVEN GERHARDT
RNA Oligo supplier??? John Storring
cDNA molecular wieghts GILLIAN NI BHARLO BOTANY PG
Announcement: 1996 Cold Spring Harbor Lab Manual Source Book nw available Joan Boyce
Pellet paint ? Paul N Hengen
- Pellet paint ? Walter Hill
- Pellet paint ? Ted M.
Commercial nonradioactive riboprobes Paul C. Grimm MD
PCR hood Bob Kodrzycki
What is "Cleavase" Dr G R Taylor
Opinions on CapFinder? John Finnerty
Use of d(AT)9 probe for detection of microsatellites Marcel Fernandez
Wanted: Peptide quantitation method W.H. Colledge
Do you know? Tess
(none) Administrador de iihema.fmed.uba.ar
gal4 strains for yeast two-hybrid Seth Corey
Culture of E.Coli (SURE) / Large Plasmids / Foreign Sequences Dr Alexander J Annala
What's behind the AmpliTaq Gold trick? Zophonias O. Jonsson
cDNA library..help CRH
- cDNA library..help Andre Renard
Screening for DNA binding protein Rich Kernan
How to gel PEG solutions? Terry Conley
- How to gel PEG solutions? Ted M.
Luciferase in Vero problems David N. Levy
streptolysin experts wanted Darren D. Browning
Getting cells off plates Microplate at eworld.com
Alternative/autoclaving for denucleasing plasticware STOP Microplate at eworld.com
plasmid cDNA library amplification CartonKJ
PFGE references? Anonymous
plasmid cDNA library amplification TJ Murphy
- plasmid cDNA library amplification Frances Hannan Zoology
RNase Protection Assay hint.. Osama
Assay of vitamins Krzysztof Treder
How to get a free copy of the 1996 CSHL Source Book JoanBoyce at aol.com
Recombinant phage antibody system Chao-Liang Wu, PhD
PFGE references? WSchick at aol.com

Last message date: Sun Mar 31 23:35:03 EST 1996
Archived on: Sun Mar 6 20:59:58 EST 2005

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