DNasing RNA samples
graham at biodec.wustl.edu
Wed May 1 10:36:56 EST 1996
If you want to heat your RNA you had best chelate all the divalent
cations, or you will get significant hydrolysis above 70C, or so I'm
I have been using a protienase K digest and organic extraction to
inactivate DNAse. I remeber having residual DNAse problem frequently
after adding it to Lambda preps in the past, hence the protease
J. Graham PhD
Washington University of St. Louis
<pkursula at cc.oulu.fi> writes:
>After you treat your RNA samples with DNase before reverse transcription,
>can you kill the DNase by heating, or will you destroy your RNA as well?
>Other suggestions on how to get rid of DNA contaminations and unspecific
>bands in RT-PCR are also appreciated.
More information about the Methods