DNasing RNA samples

James Graham graham at biodec.wustl.edu
Wed May 1 10:36:56 EST 1996

If you want to heat your RNA you had best chelate all the divalent 
cations, or you will get significant hydrolysis above 70C, or so I'm 

I have been using a protienase K digest and organic extraction to 
inactivate DNAse. I remeber having residual DNAse problem frequently 
after adding it to Lambda preps in the past, hence the protease

J. Graham PhD 
Biology Department 
Washington University of St. Louis 

<pkursula at cc.oulu.fi> writes:

>After you treat your RNA samples with DNase before reverse transcription, 
>can you kill the DNase by heating, or will you destroy your RNA as well?

>Other suggestions on how to get rid of DNA contaminations and unspecific 
>bands in RT-PCR are also appreciated.

>Petri Kursula

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