Electroblot question

Dr E. Buxbaum EB15 at le.ac.uk
Thu May 9 11:42:38 EST 1996

When the original gel has been run without SDS, you have to do the blotting either at a pH were 
the protein has lots of negative charges, or to include 0.1% SDS in the blotting buffer. The 
SDS can also help to blott very large proteins, but it increases the risk of small proteins 
sliping through the membrane (if that occurs, add some methanol to the buffer).

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