Lysing of CHO cells for western blot??

Pramathesh S. Patel Pramathesh_S._Patel at ccmail.bms.com
Tue May 21 15:39:54 EST 1996


Hi:

   What is the best method to lyse CHO cells for SDS-PAGE western blot analysis?.  I am
used to running western blot's using bacterial cells by mixing equal volumes of cells 
suspension and 2X SDS-loading buffer and boiling the samples.  Will I need to either go through 
i) several freeze-thaw cycles or ii) sonication of the CHO cell suspension to get efficent 
lysis.  
   Thank you in advance

Pramathesh

e-mail:  Patel_P at BMS.com 




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