"Gently Resuspend"

Sailesh Surapureddi Sailesh.Surapureddi at mcb.liu.se
Thu May 23 20:11:41 EST 1996


Hello there!

 I have used a 10mL pipet  for 'Gentle resupension'. All I did was rinse
the pipet 2 to 3 times in cold buffer and then  for the cells, they were
initially swirlled and then pipetted up and down 10-12 times using
powerpette on ice. All the cells were suspended properly and I got
reasonably good yeilds. So I guess this much of harsh treatment, the cells
would be able to take without crying out loud.

Cheers
Sailesh.





More information about the Methods mailing list