Methods May 1996 Archives by date

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Starting: Wed May 1 00:16:59 EST 1996
Ending: Fri May 31 22:14:56 EST 1996
Messages: 1303

Commercial source for HA-probe antibodies???? Thomas R. Anderson
What is Histidine tagging like? Tony Hodge
(none) Peter Wolstenholme
(none) NUALA MARTIN
direct PCR from E. coli Olaf Lange
extraction from sections reckon
license for TAQ john brennand
direct PCR from E. coli john brennand
slide printer Mark Blight
[solubilization of inclusion bodies with SDS?] Mark Blight
CTAB.... Mark Blight
"Motivation" for grad students Randall Willis
Amazing PCR results with TMAC John Dixon
please ignore Albrecht W. Frauendorf
Crystal structure programmes Mr. G. Morley
Do A's really fall off over time? Paul N Hengen
Edward Ampoule constrictor wanted Dr. Duncan Clark
Help! RNA isolation from Guinea Pig Liver Aslam Hassan
DNasing RNA samples James Graham
Sending bacteria via mail Jorge L. Galazzo
About ligation Chris Boyd
Help! RNA isolation from Guinea Pig Liver john brennand
Quick-hyb solutions? Mr N C J Binnie
Crystal structure programmes Ashok Aiyar
Pouring sequencing gels the old fashioned way S John
"Motivation" & Attitude Alexander Kraev
Getting rid of Proteinase K????? DANIEL Y KIM
Help--Cloning Nightmare Mucke Lab
peptide gel Achim Recktenwald
peptide gel Achim Recktenwald
license for TAQ Rafael Maldonado
TA cloning 8 kb KHUANG at CHEMVX.TAMU.EDU
Biorad Screens Elizabeth Rosenberg
Information about DNA methylase zhangxia at post.queensu.ca
Protein Induction Problem Trish Brafford
Getting rid of Proteinase K????? Dima Klenchin
Michael J. Tuvim
asymmetric pcr Clare Morland
Western Blotting Current Problem Aaron Polesky
peptide gel Stephen P. Driska PhD
Oligo binding stabilizer Brett Beitzel
PCR Hell Warren D. Kruger
Qiagen Oligotex? David Hieber
20kb plasmids Martin Schenker
Taking a protein from native PAGE to a SDS Page? Michael R. King
radioactive probe synthesis via pcr Nancy Duncan
Help! RNA isolation from Guinea Pig Liver Nancy Duncan
Protein in inclusion bodies Rita Lim
Problem in PCR xiaoming yi
Agrobacterium tDNA vectors Dr Leonard Bloksberg
{Q} Quantitative RT pcr Han Bok Kim
Protein Induction Problem Edgar Valencia
test Han Bok Kim
peptide gel Seth Corey
Unsubscribe Luiz Carvalho
unsubscribe carvalho at WUSTLB.WUSTL.EDU
PCR Hell KHUANG at CHEMVX.TAMU.EDU
radicactive probe synthesis via pcr KHUANG at CHEMVX.TAMU.EDU
Negative siver stain! Belinda Ahlers
Subcloning Suggestions ? Amna Saeed
cutting plasmids for PCR Andrew Schrader
PCR of large plasmids fjgen at okstate.edu
(none) Steve Schramm
PCR Hell Tom Dougherty
Pouring sequencing gels the old fashioned way Alexander Kraev
Qiagen Oligotex? john brennand
Problem in PCR john brennand
20kb plasmids john brennand
Getting rid of Proteinase K????? Nigel Kenward
Basic Info Chris Boyd
Qiagen Oligotex? Stefan Kappeler
cutting plasmids for PCR Michael Szardenings
His-tag protein purification Mike Rogers
pcr tubes Herta Steinkellner
Anti-LDL/alpha2-macroglobulin receptor needed Cheredeev Anatoly
Western Blotting Current Problem David Ward Rusnak
Subcloning Suggestions ? Ian Goodfellow
Western Blotting Current Problem Sean Sweeney
Quick-hyb solutions? Duke Groebe
TREE FORENSICS Steve Griffiths
Western Blotting Current Problem Aaron Polesky
good antibodies against yeast proteins? Eckhard Boles
Affinity constants Eric S. Bean, PhD
message try CKY at mail.nerc-oxford.ac.uk
message test CKY at mail.nerc-oxford.ac.uk
anyone know how large (in kb) the canine genome is? Vid Mohan-Ram
PCR of large plasmids Richard J. Dudley
PC12 cell line IAN MCFARLANE
hemopexin antibodies??? Mr. D.M. Shaw
NON-RADIOACTIVE NORTHERNS Lisa Smith
Commercial source for HA-probe antibodies???? Jen Logan
immunopptn R. Woodward
microsomes in IVT R. Woodward
PCR Hell Shelley Cole
POINTER TO METHODS FAQ LIST Paul N Hengen
His-tag protein purification Paul N Hengen
v-abl and phosphatase assay sam simons
microsomes in IVT HIRANYA ROYCHOWDHURY
help with semiQ RT-PCR Ed Castro
"Motivation" for grad students Paul N Hengen
Subcloning Suggestions ? namy
Thermo RT's & cDNA library (hi G+C) James Graham
tissue fixatives for in situ hybridization at cencom.net
Protein Elution from Nylon auster
PCR-Mimics- construct kit-Internal Controls for Quantitative PCR Todd Miller - Pharmacology
Screening library: host has homologue of target radomski
(no subject) manda rice
"Affinity constants" Andrei Popov
Western Blotting Current Problem Ron Tate
Amazing PCR results with TMAC vilimf01 at mcrcr6.med.nyu.edu
Taking a protein from native PAGE to a SDS Page? Ron Tate
neutralizing TGF beta Danny Altshuler
radioactive probe synthesis via pcr Michael W. Thompson
Wanted-pET vector host with B-gal/X-gal assay Richard Waring
PCR Hell Warren D. Kruger
Western Blotting Current Problem Michael W. Thompson
HELP: Shrews neaded Gordan Lauc
PCR Hell vilimf01 at mcrcr6.med.nyu.edu
Inoue Comp Cells Brett Beitzel
Affinity constants Dima Klenchin
Affinity constants Keld Sorensen
Negative siver stain! Boris Lenhard
Biometrical Question Chi-Kuang Wen
Western Blotting Current Problem Keld Sorensen
Cycle Sequencing Problem Carolyn Calnan
Nonisotopic Labels, Suggestions? Michael Oda
"Motivation" for grad students Kiley R. Prilliman
His Tag constructs ??? Eric Prossnitz
pZErO vectors David Groth
Sending bacteria via mail Ferland Louis H.
Signal peptide sequence Slavica Masina
FLAG-tag from Kodak Ted M.
Intron prediction web site? Marc Rosoff
mRNA isolation Himadri K. Samanta
phosphatase Shur lab
Plate Readers Paul Herron
Rep (2) : PCR-Mimics- construct kit-Internal Controls for Quantitative PCR Mr SHIRE David
is there a good website for multiple-aligning of sequences in combination with own? Ruud Ross
Xma I problems, please help!!! Jose F Gutierrez Marcos
Inoue Comp Cells Alexander Kraev
high GC PCR PRETORIUS
Cycle Sequencing Problem Alexander Kraev
Affinity constant Andrei Popov
(no subject) john brennand
Problems with Qiagen-Giga-prep Andrea Bernhaupt
direct PCR from E. coli Dr. Duncan Clark
pZErO vectors Michal Svoboda
HELP: Shrews neaded Keld Sorensen
pZErO vectors Steven Goldberg
macrophages depletion Myriam Onno
Ni-NTA Spin kit ....HELP Thorarinn Blondal
Re-use of electroporator cuvettes GILLIAN NI BHARLO BOTANY PG
RE- pZERO vectors Brett Beitzel
direct PCR from E. coli Kevin J. Laddison
license for TAQ Tracy Aquilla
Differential Display kit ?? Glen Shearer
Rat Ab protocol ? reichhart at astorg.u-strasbg.fr
shooting yourself in the foot.... Tracy Aquilla
Getting rid of Proteinase K ????? Your name
Cloning into pET15b James Smith
poly-His antibodies from Qiagen: is it good ? Eric Campeau
radioactive probe synthesis via pcr Daniel Pereira
"Motivation" for grad students Paul N Hengen
Large scale transfections Eric B. Carstens
Source target_random-hex primer? James Graham
Baculovirus Question - Pluronic Tim Angelotti
RNAse Protection Assay William Coetzee
MICROSATTELITES IN PLANTS Steve Griffiths
Software for strain collections Ash, JosŽe
(none) Lorraine M. Rellick
(none) Lorraine M. Rellick
Western Blotting Current Problem Ralf Breuker
20kb plasmids Graham Dellaire
Re-use of electroporator cuvettes Kiley R. Prilliman
Re-use of electroporator cuvettes corboy at utdallas.edu
**HELP!** Plant growth medium contamination c635499 at black.missouri.edu
immunopptn Bernard Murray
A Yeast Housekeeping Gene for Northern? Namjin Chung
help with semiQ RT-PCR Bernard Murray
Screening library: host has homologue of target Shaun Tyler
carbenicillin Catherine DeLong
Western Blotting Current Problem Thomas Thekkumkara
direct PCR from E. coli Dr. Peter Gegenheimer
concatamers...help John Korte
TEST feng yang
Subcloning suggestions Amna Saeed
Capillary transfer from polyacrylamide-gels Jerry Kropp
T4 polynucleotide kinase and ligation efficiency Jeremy Chen
T4 polynucleotide kinase and ligation efficiency Ned Mantei
Getting rid of Proteinase K????? Arthur Wohlwill
Troubles purifing DNA with QIAEX II Yaron Lidor
Do A's really fall off over time? Simon Dawson
Sequencing quotes 2 gerry oneill
Ni-NTA Spin kit ....HELP Tara McCready
Signal peptide sequence Gregg B Wells
Sending bacteria via mail Ferland Louis H.
radioactive probe synthesis via pcr Ferland Louis H.
Cyanobacterial RNA Youich Kondou
Nickel agarose for His tag (fwd) Hung-yiu Ho
EtOH + PBS = precipitation? (fwd) Hung-yiu Ho
Quick Protein Concentration Assays (fwd) Hung-yiu Ho
What is Histidine tagging like? Zophonias O. Jonsson
Quick Protein Concentration Assays (fwd) Dima Klenchin
is there a good website for multiple-aligning of sequences in combination with own? ege
microsomes in IVT Michael Coady
All plaques are positive KHUANG at CHEMVX.TAMU.EDU
Yeast mutant list KHUANG at CHEMVX.TAMU.EDU
Info re Cadgene? Peter Wang
Info re Cadgene? milstone
Q: Properties of Phospho-cellulose (P11, Whatman) Dima Klenchin
Cyanobacterial RNA Edgar Valencia
Nonisotopic Labels, Suggestions? Joan Boyce
T7 in vitro transcription: problem template Bernard Murray
Electroblot question rof
Ni-NTA Spin kit ....HELP Mike Rogers
Universal mouse oligo? Eivind Hovig
New Company Formed - Looking for People Glenn Diamond
DNasing RNA samples jms93
Qiagen PN Buffer???? Mary P. Remington
direct PCR from E. coli jms93
Help! RNA isolation from Guinea Pig Liver jms93
Wanted: Articles about your Career Experiences in Science DrHeasley
Membrane-impermeable Reducing Agents Michael Daws
Dichloromethane vs. Chloroform Glen Gaughan
Q: Properties of Phospho-cellulose (P11, Whatman) Randall Willis
immunopptn Keld Sorensen
bcl-2 cDNA probe Jeffrey Upperman
license for TAQ Robert G. Hamilton
Ovalalbumin 45 kD Marker Problems Greg Davis
Good mutatgenesis kit--opinions Steven Goldberg
Source for recombinant murine Interleukin-12 ? Dirk Seegert
Subcloning suggestions Hayssam Zakaria
cutting plasmids for PCR Jody K. Hirsh
microsomes in IVT Jody K. Hirsh
Software for strain collections Sigurður E. Vilhelmsson
Amazing PCR results with TMAC James Graham
No. of bases to cut terminal RE site? Jody K. Hirsh
"Motivation" for grad students James Graham
No. of bases to cut terminal RE site? Josee Ash
Protein in inclusion bodies Song Tan
shooting yourself in the foot.... Bill Alexander
Re-use of electroporator cuvettes Paul N Hengen
No. of bases to cut terminal RE site? Richard J. Dudley
Differential Display kit ?? Tonja Burshek
Source of Murine IL-12 Joan Boyce
Direct PCR of yeast clones picked from agar plate? Namjin Chung
Subject: carbenicillin Joan Boyce
Sending bacteria via mail Paul N Hengen
2-D gels Anonymous
Getting rid of Proteinase K ????? James Graham
Immunofluorescence markers Yun C. Chang
Yeast mutant list Maria C. Costanzo
Q: Properties of Phospho-cellulose (P11, Whatman) matt thomas
A Yeast Housekeeping Gene for Northern? Brett Burkholder
help with semiQ RT-PCR Heinz-Juergen Schaefers
help with semiQ RT-PCR Heinz-Juergen Schaefers
pls read both of my replies (RE:help with semiQ RT-PCR)... Heinz-Juergen Schaefers
bcl-2 cDNA probe Richard Moldwin
Thermal Cyclers Terry Conley
Xma I problems, please help!!! ed_marsh at stratagene.com
license for Taq Jill Kellogg
Cloning into pET15b Brian Carey
license for TAQ Rafael Maldonado
Help--Cloning Nightmare Rafael Maldonado
Direct PCR of yeast clones picked from agar plate? Darren Schneider, MD
Help--Cloning Nightmare Rafael Maldonado
primer design Shi-Jye Chu
AP2 location Karen Kroeger
Ets expression vector Karen Kroeger
Inoue Comp Cells Jeremy Marcus
Direct PCR of yeast clones picked from agar plate? Jeremy Marcus
Wanted:" Commercial Nuclear Extract Stefan Einar Stefansson
primer design LAU Tze Chin
Source of Murine IL-12 Dirk Seegert
sequence of pmcNeo/pmcTK Cheah Kathryn Song Eng
carbenicillin john brennand
is there a good website for multiple-aligning of sequences in combination with own? Dirk Seegert
Inoue Comp Cells Song Tan
Human DHFR clones? Fourie Joubert
Meeting Announcement: Gene Amplification and Detection Cambridge Healthtech Institute
help! how much % ethanol inhibits RNase A? Zhengyang Wu
Re-use of electroporator cuvettes John Dixon
Plate Readers Keld Sorensen
Direct PCR of yeast clones picked from agar plate? Steven Goldberg
My insert is kicked out of my vector Lars Vahlkamp
license for Taq Robert G. Hamilton
Wanted: Commercial Nuclear Extract Stefan Einar Stefansson
Q: Properties of Phospho-cellulose (P11, Whatman) Giovanni Maga
Can one quantify with In situ PCR? Paul Brian Scruggs
Q: Properties of Phospho-cellulose (P11, Whatman) Randall Willis
BCL primers Agnieszka Szczepek
Review Antibody Production Lena Brydon
Plate Readers Joan Boyce
Help me to find an author Gabriela Martinez Trevi o
sequence of pmcNeo/pmcTK Shi-Jye Chu
Help me to find an author please Gabriela Martinez Trevi o
Help me to find an author please Gabriela Martinez Trevi o
Affinity constants David Moss
Affinity constants Dima Klenchin
Intron prediction web site? Angel Zarain
Problems with pCDNA3 Angel Zarain
Small molecules & nitrocellulose Louis Hom
Re-use of electroporator cuvettes Paul N Hengen
E. coli nitroreductase antibody S.D. Gorman
Inoue Comp Cells Alexander Kraev
Dioxin or "aryl hydrocarbon" source Dennis Templeton
Software for strain collections SL Forsburg
Problems with Qiagen-Giga-prep Lina Bueno
Properties of Phospho-cellulose (P11, Whatman) Karen Browning
Direct PCR of yeast clones picked from agar plate? Dean H. Saxe
My insert is kicked out of my vector ijiwaru at wheel.dcn.davis.ca.us
primer design Namjin Chung
Who makes pML? Anton Tutter
Who makes pML??? Anton Tutter
PCR Hell Sara Carlisle
uncut vector DNA Kalyanaraman
Hoefer TKO 100 Mini-fluorometer H. Woo
Texas red vs FITC H. Woo
license for TAQ Tracy Aquilla
Can one quantify with In situ PCR Agnieszka Szczepek
Antibody purification/GST-protein Michael J. Tuvim
Direct PCR of yeast clones picked from agar plate? Kevin Morano
Small molecules & nitrocellulose Dom
Dioxin or "aryl hydrocarbon" source Arthur Wohlwill
Differential display information Wei-Young Wang
How to clean a tray of BCIP/NBT precipitate? Pam Snyder
Bio Rad any good? Pam Snyder
plasmid PTIS1DT hassane mchaourab
radioactive probe synthesis via pcr Ferenc Marincs
soybeans - promoters and transformation methods Bill Langridge
Sequencing large DNA by transposon Kah Tong, Seow
antisense Dirk Van Oekelen
liposomes/membrane fusion Jurgen Vanhauwe
Dioxin or "aryl hydrocarbon" source Bernard Murray
CAT GENE SUBSTRATE fernanda at uctvms.uct.ac.za
dephosphorylation Xiaohai Gong
liposomes/membrane fusion Jurgen Vanhauwe
Inoue Comp Cells Rico Laage
Vertical Agarose gels, help Inmaculada Garcia
Vertical Agarose gels, help Alexander Kraev
Q: How to extract DNA from spleen tissue? Jimmy Rail
Production of ss phagemids AJ. Murray
DNA from herbarium material J. G. Gibbings
Qiagen PN Buffer???? jms93
STUTTERING MICROSATELLITES! Timothy R Jackson
Ni-NTA Spin kit ....HELP sam simons
Direct PCR of yeast clones picked from agar plate? francois GENDRE
non-radioactive immunoporeicitation Howard T. Petrie
Supercoiling assay with topoisomerase Jeffrey Atkinson
is there a good website for multiple-aligning of sequences in combination with own? Travis
Sequencing large DNA by transposon Robert G. Hamilton
HELP!!! Shrews desperately needed! Gordan Lauc
A method to mark plasmids? Iacob Mathiesen
No. of bases to cut terminal RE site? Mark R. Miglarese
pLM1? KHUANG at CHEMVX.TAMU.EDU
Soybean KHUANG at CHEMVX.TAMU.EDU
(no subject) Benjamin Charvat
threonine kinase substrates Mr. M Uden
Vertical Agarose gels, help Dennis Templeton
Western Blotting Current Problem Michael Daws
My insert is kicked out of my vector Georg Kroeger
XbaI Methylase source? John Dixon
3' end-labelling help needed Anton Tutter
CAT GENE SUBSTRATE fernanda at UCTVMS.UCT.AC.ZA
(no subject) Farid jalali
Request for PC-12 cell line Nobu Kikyo
Antibiotic resistance cassettes or cartridges? Farid jalali
Uncut vector DNA-again David A. Bailey
HL60 Transfection L. Fliegel
Getting rid of Proteinase K????? Hector Cruz-Lopez
HELP: 5' RACE sa95078
Carbenicillin Debra Schuster
radioactive probe synthesis via pcr Rob Jordan
Sequencing large DNA by transposon Rob Jordan
HELP!...Peptide synthesis Chanan Angsuthanasombat - ST
RU 486 geoff doerre
Direct PCR of yeast clones picked from agar plate? gerry oneill
STUTTERING MICROSATELLITES! juleen.cavanaugh at anu.edu.au
DNasing RNA samples James Graham
help! how much % ethanol inhibits RNase A? James Graham
Inoue Comp Cells James Graham
Having problems with Ambion Hybspeed RNase protection kit. Scott Hodgson
QuickChange mutagenesis kit paul ealing
Small molecules & nitrocellulose Dan Brose
DNasing RNA samples K J C 944
rat actin-b mRNA Yasuhito Abe
Problems with Qiagen-Giga-prep Yasuhito Abe
anti-jun antibodies Thomas R. Anderson
Australian agent for Hoefer H. Woo
Supercoiling assay with topoisomerase Richard J. Reece
Help--Cloning Nightmare Ferland Louis H.
enterokinase cleavage CDMENU at ccs1.cc.monash.edu.au
HELP. DNA isolation for PCR from fish shell ausl
ECL kit MV. Hayes
3' end-labelling help needed john brennand
How to clean a tray of BCIP/NBT precipitate? G.P.
CRYSTAL STRUCTURES Mr. G. Morley
Signal peptide sequence Jose Casal 'Lince'
Nucleospin buffer composition Andrew J. Roger
EZ-PCR help Mick N. Mulders
(none) maeno at NCNAXP.NCNP.GO.JP
DNA staining (non toxic) William Sofer
PCR sequencing of chromosomal DNA ezra yagil
Anti GAPDH antibody anyone? Dr R.V. Giles
restriction sites Brian A. Flannigan
How to clean a tray of BCIP/NBT precipitate? Matthew J. F. Waterman
Differential display information Tom Dougherty
Bio Rad any good? Marc Visconti
My insert is kicked out of my vector Tom Dougherty
Intron prediction web site? Carlos Polanco
TA cloning 8 kb Carlos Polanco
Taking a protein from native PAGE to a SDS Page? Dr E. Buxbaum
Electroblot question Dr E. Buxbaum
DNA staining (non toxic) Paul N Hengen
ECL kit Paul N Hengen
ultracentrifugation - cs/cl purification HELP! Erythrochaetophile
Vertical Agarose gels, help Paul N Hengen
Cloning into pET15b Maria C. Costanzo
Chicken antibody purification M. Aitchison
RU 486 Keld Sorensen
don't read, this is just a test Lina Bueno
Antibiotic resistance cassettes or cartridges? Lina Bueno
Question about a DNA Binding assay ccc6 at Lehigh.EDU
Cell lines for transfecting Dima Klenchin
DNA staining (non toxic) Bill Alexander
How to clean a tray of BCIP/NBT precipitate? Michael J. Prigge
Bio Rad any good? HIRANYA ROYCHOWDHURY
library construction? Mike Curtin
Dioxin or "aryl hydrocarbon" source Geoffrey D. Wheelock
DNA staining (non toxic) Barclay Morrison III
Western Blotting Current Problem julian catmull
Plasmid PTIS1Dt hassane mchaourab
Antibody purification/GST-protein julian catmull
Cell lines for transfecting John Ladasky
(none) Keld Sorensen
(none) Ken Howe
DNA staining (non toxic) Bernard Murray
Cell lines for transfecting Dima Klenchin
voltage to temperature? Eurie Lee
Dioxin or "aryl hydrocarbon" source Jimbo
How to clean a tray of BCIP/NBT precipitate? Rico Laage
ECL kit RORY O'BRIEN (ZOOLOGY) PG
Vertical Agarose gels, help Zophonias O. Jonsson
carbenicillin Dr I.S. Viney
Question about a DNA Binding assay Dirk Seegert
Production of ss phagemids P. Mertens
Neomycin phophotransferase Antibody mkennedy at chmeds.ac.nz
Colloidal Gold Protein Stain Mark Blight
Degenerate primers for protein domains Gene Huh
RNA-labeling PC16 at
Salary of a senior scientist Muhammad A. Lodhi
western Francois Galgani DEL-EX
help! how much % ethanol inhibits RNase A? Enrique Graziano-Areste
Vertical Agarose gels, help Dennis Templeton
Hoefer TKO 100 Mini-fluorometer Alexander Kraev
HELP with Versican Seq. Brian S. Thoma
Use gp32 SSB in G+C rich templates? James Graham
Haviland_Hamel_Leach_Roe_Nash_Coady_Clark_Hengen? James Graham
staining protocols for cancer samples Jaron Collis
Question about a DNA Binding assay Paul N Hengen
HELP: 5' RACE M.W. SMITH
Question about a DNA Binding assay / fragment size Heinz-J. Schaefers
HELP: 5' RACE Enrique Graziano-Areste
DNA staining (non toxic) Heinz-J. Schaefers
rat actin-b mRNA Heinz-J. Schaefers
fluorescence polarisation/anisotropy springer
Membrane-impermeable Reducing Agents corboy at utdallas.edu
Lambda EMBL3A pauling at DARWIN.UCSC.EDU
Hyb solutions chriso at dfrc.wisc.edu
RT PCR / Kruger,Cole Wayne M. Barnes, Ph.D.
Use gp32 SSB in G+C rich templates? Paul N Hengen
promoter analysis martin LEACH
Chemiluminescent Detection Kits martin LEACH
cloning of YAC DNA martin LEACH
LacSwitch Dong Yin
ECL kit Paul N Hengen
ECL kit Paul N Hengen
ECL kit Anthony M. Dubin
Sequencing large DNA by transposon prscholl
peptide display/E. coli First M. Lastname
Subject: Colloidal Gold Protein Stain Joan Boyce
Source Ribonuclease T2 PNorton
RT PCR / Kruger, Cole Wayne M. Barnes, Ph.D.
denaturing dna on southern blot Lely Chow
Do A's really fall off over time? Lely Chow
Bio-Rad Seq units Jerry Kropp
Molecular Embryology and Transgenic Technology Cheah Kathryn Song Eng
ss vs ds DNA Bryan L. Ford
Salary of a senior scientist DrHeasley
Ni-NTA Spin kit ....HELP Michael Szardenings
Vector pUHD.10-3 Ian A. York
restriction sites Klaus Salger
problems with pET11 Tanel Pungas
Texas red vs FITC ming at msvax.mssm.edu
promotor analysis: info please!!! Jose F Gutierrez Marcos
Western blots KRTurbyfil
restriction sites Daniela Sciaky
PFGE (Pulsed-Field Gel Electrophoresis) Josh Goldstein
RNA without DNA Robert Phelps
mouse tail DNA extraction kits Paul Kalitsis
Molecular genetics of pepper ? mhlee
PFGE Andrei Popov
XbaI Methylase source? Michael Szardenings
PFGE Andrei Popov
FISH and CGH need protocols Isabelle Camby
PFGE Darren Schneider, MD
Assembly software for many sequencings Hiroshi Inoue
salmon sperm DNA Szabadkai Gyorgy
Direct PCR of yeast clones picked from agar plate? AShaw5
Affinity constant AShaw5
Electroblot question AShaw5
PC12 cell line AShaw5
Odd PCR artifact AShaw5
Molecular Embryology and Transgenic Technology Cheah Kathryn Song Eng
mouse tail DNA extraction kits Lo Wing Ip Anthony
Asking receptors expressed on COS cell membranes YONG-SUNG JUHNN
Degenerate primers for protein domains Peter Wang
PFGE (Pulsed-Field Gel Electrophoresis) WSchick at aol.com
Recovery of proteins from blotted membrane yongsungkim
Recovey of protein from blotted membrane yongsungkim
Recovery of protein from blotted membrane Kernee H. Chang
DNA staining (non toxic) Bryan L. Ford
Nucleospin buffer composition Edward Wang
salmon sperm DNA Edward Wang
DNA staining (non toxic) Jurgen Vanhauwe
dialysis Jurgen Vanhauwe
chinese mother x african father----> mtDNA profile of child (chinese / african)????? Vid Mohan-Ram
mouse tail DNA extraction kits Gary Kucera
Vector pUHD.10-3 Richard Moldwin
RT PCR / Kruger, Cole Warren D. Kruger
Where do I find Hydrolink-MDE? Robbert Slebos
Nucleospin buffer composition martin LEACH
Assembly software for many sequencings martin LEACH
Assembly software for many sequencings Michael Lassner
salmon sperm DNA martin LEACH
dialysis martin LEACH
Source for 1 cm gap electroporation cuvettes? Ashok Aiyar
Can one quantify with In situ PCR Paul Brian Scruggs
Primer-dimer formation Chris Dambrowitz
Qiagen columns T. Moore
PFGE (Pulsed-Field Gel Electrophoresis) RZINKEL at promega.com
ExonucleaseI/Alk Phos Rx of PCR products Scott Vande Pol
Australian agent for Hoefer Richard J. Dudley
RDA researchers Edward Wang
Qiagen columns Ferland Louis H.
Does 6-His inhibit protein's activity? Travis
ExonucleaseI/Alk Phos Rx of PCR products jane staunton
Tm for mutagenic primers! FirstName LastName
Ovalalbumin 45 kD Marker Problems Iain Wilson
6-His sequence lost during PCR Travis
Ni-NTA Spin kit ....HELP Dima Klenchin
mouse tail DNA extraction kits Bernard Murray
Primer-dimer formation martin LEACH
Small molecules & nitrocellulose James Sigafoos
dialysis Mayumi Yagi
Quantitating RNA Mayumi Yagi
salmon sperm DNA jfh
dialysis martin LEACH
HELP HELP HELP needed with RNA extraction Eric Sarnighausen
Ni-NTA Spin kit ....HELP Michael Szardenings
Aminotiazole Plates John Stebbins
New Reference Web Site Christian Carollo
Membrane-impermeable Reducing Agents Kelly Ambler
Help on sample size calculation H. Woo
help Paula Padula
(no subject) Paula Padula
HELP HELP HELP needed with RNA extraction Salia Gherras
EtBr in partial RE digests Marcia MacDonald
Western Blotting Current Problem Salia Gherras
Anybody ever tried PCR with a "bubble" primer? Feng Yang
(none) hawkins
DNA from herbarium material Michelle Gleeson
Australian agent for Hoefer Michelle Gleeson
How to remove Commossie Brilliant Blue Leo
Electroporation and laryngeal epithelial cells? Kari Asikainen
EtBr in partial RE digests Zophonias O. Jonsson
salmon sperm DNA and Northern again Szabadkai Gyorgy
EtBr in partial RE digests Ned Mantei
Mung Bean or S1 nuclease ??? john brennand
hybond-N namy
EtBr in partial RE digests milstone
Assembly software for many sequencings Christoph Grunau
Problems with Qiagen-Giga-prep Zhengyang Wu
Where do I find Hydrolink-MDE? Mark Freeman
Problem: GST-Fusion protein is Insoluble... Purification???? K. Cole
Quantum Yield of Ethidium Bromide? Tehcnical Assistance
RNA quantification human skeletal muscle Edouard Lauzier
library construction? Steven Goldberg
Direct PCR of yeast clones picked from agar plate? Francois Galgani DEL-EX
Source for Hepatic Nuclear Factor 3 <alpha> Antibody? Alice Simmons
Plasmid drawing program Steven Theg
Quantitative rt-PCR HELP!!! Gordon Allison
SacI in MCS--Euk. Vector? Jody K. Hirsh
Brassica protoplast transfection Z. Jeffrey Chen
Where do I find Hydrolink-MDE? (fwd) Neil Harris
***Desparate for cDNA Library ***** Hirdey Bhathal
Australian agent for Hoefer Anthony J. Murray
dialysis Richard Van Frank
Affinity constants Achim Recktenwald
PCR problems using BMB Expand DKane
Problem: GST-Fusion protein is Insoluble... Purification???? ijiwaru at wheel.dcn.davis.ca.us
Australian agent for Hoefer Keld Sorensen
Single Strand PCR....Help... Conditions??? Ivan Brukner
denaturing dna on southern blot Gregide
replication in vitro Ivan Brukner
Quantitating RNA Tonja Burshek
formaldehyde-agarose gels nsaunders at molbiol.ox.ac.uk
Plasmid drawing program Michael Wong
RT-PCR, BCL2 PROBLEM Anonymous
(no subject) ppadula at IMPSAT1.COM.AR
(none) martin LEACH
(no subject) martin LEACH
Hae I?? Jillian Johnston
quantitation of dilute DNA Shelley Cole
FACS-friendly antibodies for placental alk phos Glenn Cantor
*****Desperately need a mouse cDNA library***** Hirdeypal S. Bhathal
Primer-dimer formation Ed Castro
test Sanja Samac
PFGE (Pulsed-Field Gel Electrophoresis) Mike Finney
Human fetal DNA Sanja Samac
bcl-2 cDNA probe kevin gilbride
fluorescent anti-fade reagents hawkins
Recovey of protein from blotted membrane kevin gilbride
Anybody ever tried PCR with a "bubble" primer? Rafael Maldonado
Thermal Cycler for PCR ***
home made RACE? Michael H. Bracey
Where do I find Hydrolink-MDE? (fwd) Neil Harris
Re-use of electroporator cuvettes Yves HATZFELD
What is NTCB that cleaves proteins Juha Saharinen
Help! Bacterial recombination in recA- cells Narendra Yadav
Amino Acid Analysis Rajesh Krishnamurthy
[Q]: best restrictase for PCR cloning Ivo Wiesner
Plasmid drawing program Jim Woodgett
Primer-dimer formation Douglas Prasher
Anybody ever tried PCR with a "bubble" primer? Sultan Kalifa
PM2 DNA source? Brett Beitzel
EtBr in partial RE digests Paul N Hengen
Mung Bean or S1 nuclease ??? Bernard Murray
How to clean a tray of BCIP/NBT precipitate? Paul N Hengen
Where do I find Hydrolink-MDE? Robbert Slebos
home made RACE? David Micklem
double-stranded RNAse Takahiko Ito
Help! Bacterial recombination in recA- cells (fwd) Edgar Valencia
Protocol for making GSH affinity matrix ? atown at MEDCENTER.WPMAIL.WFU.EDU
Hae I?? Mikhail Alexeyev
salmon sperm DNA J. Pat Martinez
How to remove Commossie Brilliant Blue Arthur Wohlwill
clontech one-hybrid system..any users??? John Brunstein
PCR problems using BMB Expand Wayne M. Barnes, Ph.D.
Qiagen columns Andrea Crampton
home made RACE? orlando dominguez
PCR Jump Station Hugh Griffin
Insulin cDNA mark throsby
Is this called Southern hybridization or Northern hybridization? Zhongguo Xiong
Delta fingerprinting David Tak Yan Yu
double-stranded RNAse Edwin ten Dam
Heat DNA Degradation gdavis at uoknor.edu
Anybody ever tried PCR with a "bubble" primer? Sandhya Nerurkar
HELP: CHLAMYDIA DNA WANTED!! Julie Logan
HELP HELP HELP needed with RNA extraction 209_user_curtis
Yeast GST expression vector system PereraS
microwaving DNA??? RMCMAH93 at IRLEARN.UCD.IE
Transfection of primary fibroblasts Charles Murry
(no subject) ppadula at IMPSAT1.COM.AR
Help! Bacterial recombination in recA- cells (fwd) Edgar Valencia
Info on Paclobutrazol, an inhibitor of GA3- requested veera
Help needed on TAQMAN by Perkin Elmer (Question about filters) Maxim V. Myakishev
formaldehyde-agarose gels nsaunders at molbiol.ox.ac.uk
P-glycoprotein MDR antibody c608660 at mizzou1.missouri.edu
Qiagen columns julian catmull
microwaving DNA??? (fwd) Edgar Valencia
salmon sperm DNA your name
SDS denaturing before immunolocalization? Alan H. Schulman
RNaseH or RandomPrime 2nd cDNA? James Graham
Microwaving DNA??? Edgar Valencia
library construction? Chi-Kuang Wen
DNA rearrangements Chris Paszty
Plasmid drawing program Richard Daines
Collaborative Research contact no. Watts Robin May
Human fetal DNA Jim Cummins
What is NTCB that cleaves proteins?? Juha Saharinen
Mol Biol Faculty Positions Canada Dr. John J. Heikkila
Quantitative rt-PCR HELP!!! Olivier Gandrillon
Is this called Southern hybridization or Northern hybridization? Olivier Gandrillon
anti-IL5R alpha monoclonals Peter Czabotar
Qiagen columns Ferland Louis H.
Primer-dimer formation Ferland Louis H.
Mung Bean or S1 nuclease ??? john brennand
Is this called Southern hybridization or Northern hybridization? Ferland Louis H.
Desperate for a probe: human IgK locus intron Andrei Popov
Can I salvage my library? John Dixon
Human fetal DNA Raymond Dalgleish
Comparative genomic hybridization Isabelle Camby
Thermostable DNA ligase in DNA subcloning FABIAN MOEBIUS
Differential Display on Agarose Gels dudy bar-zvi
formaldehyde-agarose gels James Graham
test a at b.c.d
mutation rate of +type MoMuLV RT John Dixon
DNA rearrangements Chris Paszty
Differential Display on Agarose Gels przemko
immunohistochemistry technique Alan H. Schulman
Is a 50 base probe enough for Southern blotting? bjh8 at Lehigh.EDU
Help with PCR library screening Dr. Miguel A. Gomez Lim
Hoefer TKO 100 Mini-fluorometer James Graham
Nucleospin or Qiaprep spin buffers Andrew J. Roger
Qiagen columns Robot Boy
Tissue storage requirements lcwilliams at medusa.unm.edu
Antibody purification/GST-protein Anthony B. Russell
Help) Higromycine dose in establishing permanent cell line Dima Klenchin
cleaning up my probes Jennifer Wiedenman
cleaning up my probes Narasimham Jammi
rat actin-b mRNA Ed Castro
Antibody purification/GST-protein Anthony B. Russell
denaturing dna on southern blot Ed Castro
6-His sequence lost during PCR Ed Castro
Thermal Cycler for PCR Ed Castro
Is a 50 base probe enough for Southern blotting? Edgar Valencia
Agarose John Korte
pre-immune sera Jennifer Vande Weghe
Mung Bean or S1 nuclease ??? Bernard Murray
TEST-IGNORE IT Sanja Samac
cleaning up my probes Matthew J. F. Waterman
Rejected messages Edgar Valencia
Nucleospin or Qiaprep spin buffers Keld Sorensen
DNA rearrangement Chi-Kuang Wen
Help RE DEAE/Dextran COS transfections! runting at wehi.edu.au
Help: T-tailing of vectors Gabre Kemp
Hoefer TKO 100 Mini-fluorometer James Graham
Is a 50 base probe enough for Southern blotting? martin LEACH
Rejected messages martin LEACH
library construction? James Graham
blast.client under linux BOFH
Help) Higromycine dose in establishing permanent cell line Kim Myung Jong
pUC 13 lgbrieba
pUC 13 Ashok Aiyar
How to Freeze/Unfreeze/Revive blood lymphocytes? Maxim V. Myakishev
Hae I?? Ferland Louis H.
Comparative genomic hybridization Gizbab
Methoxyflurane S.D. Mottram
Help: Need to get single stranded DNA.. Friends are good medicine
Differential Display on Agarose Gels Olivier Gandrillon
Rejected messages Olivier Gandrillon
Recovery of protein from blotted membrane Dr E. Buxbaum
Plasmid drawing program Bob Horton
PCR problems using BMB Expand Bob Horton
XL1-Red cells Paul Doesburg
Colloidal Gold workshop at dnatech.com
Ligations that are inhibited by adding insert Harry J. Witchel
Marathon cDNA PCR NOLLIE
Amino Acid Analysis John E. Fox
Help Swissprot to SURVIVE Olivier Gandrillon
Delta fingerprinting Paul C. Grimm MD
"Strep Tag" Vector? Tehcnical Assistance
Ligations that are inhibited by adding insert Richard P. Grant
Hae I?? Mikhail Alexeyev
Does 6-His inhibit protein's activity? Steven Goldberg
Rejected messages Keld Sorensen
Uni-directional deletions Vinod Asundi
Ligations that are inhibited by adding insert Matthew J. F. Waterman
Source of macaloid David C. Logan
vector source Kathleen Hopp
Does 6-His inhibit protein's activity? Achim Recktenwald
Ni-NTA Spin kit ....HELP Achim Recktenwald
pcr templates--linear or supercoiled? Christopher Schultz
Rejected messages Edgar Valencia
READ CAREFULLY. SWISSPROT DATABASE and other EXPASY SERVICES will be DISCONTINUED!! Edgar Valencia
Rejected messages Richard P. Grant
Protocol for GSH affinity matrix Alan Townsend
Protocol for GSH affinity matrix Alan Townsend
COS Electroporation Questions Zach DelProposto
Do you have a lab web page? Bob Horton
Ligations that are inhibited by adding insert Harry J. Witchel
salmon sperm DNA Rafael Maldonado
May EtBr affect sequencing? Rafael Maldonado
LA PCR band - unclonable ? M.E. Hudson
pUC 13 Michael Benedik
AT cell transfection liyan SUN
Rejected messages Kiley R. Prilliman
RACE advice request Geoffrey Neale
HELP!...Peptide synthesis Chanan Angsuthanasombat - ST
Help RE DEAE/Dextran COS transfections! Bernard Murray
COS Electroporation Questions brett
Protocol for GSH affinity matrix Randall Willis
Rejected messages David Kristofferson
cutting plasmids for PCR Bret Sellman
Good mutatgenesis kit--opinions Bret Sellman
enterokinase cleavage Bret Sellman
dialysis Bret Sellman
cleaning up my probes Klaus Salger
PCR templates--linear or supercoiled Jill Kellogg
[Q]: best restrictase for PCR cloning Killerhippie Volker
In Search of GAL4-Luciferase Jeffrey Paichin Lin
Quantifying mRNA on Northerns CAShug
Do you have a lab web page? Dima Klenchin
Tissue storage requirements Mayumi Yagi
Identification of Bacillus species? Han Bok Kim
PCR templates--linear or supercoiled Karl Voss
[Q] Labeled oligo gel purification alternatives? (Qiagen?) Mike Chin
(none) J. Ibave
Source of macaloid Ned Mantei
[Q] Labeled oligo gel purification alternatives? (Qiagen?) Ashok Aiyar
(no subject) Joerg Jacoby
Looking for Sulfometuron Methyl Joerg Jacoby
Westerns, biotin-avidin and spurious bands crbaker
quantitative PCR? Hanbok Kim
**Heat shocked maize cDNA expression lib: wanted** dinakar bhattramakki
5' RACE of Long RNA's stvkoury at acsu.buffalo.edu
RNA without DNA Li-Lan Li
Tissue storage requirements vilimf01 at mcrcr6.med.nyu.edu
mutation rate of +type MoMuLV RT Karl Fischer
"Strep Tag" Vector? Tom Vink
cleaning up my probes DANIEL Y KIM
Preparative electrophoresis Adriano Savoini
RNA without DNA James Graham
Free medline searching!? martin LEACH
READ CAREFULLY. SWISSPROT DATABASE and other EXPASY SERVICES will be DISCONTINUED!! martin LEACH
Help me to find an author or the article please Gabriela Martinez Trevi o
Help me to find an author or his article please Gabriela Martinez Trevi o
shrews DESPERATELY needed... rhubner at molbiol.ox.ac.uk
RNA without DNA... rhubner at molbiol.ox.ac.uk
mutation rate of MMLV-RT... rhubner at molbiol.ox.ac.uk
Heat DNA Degradation Dr A.H.M. Van Vliet
Heat DNA Degradation Dr A.H.M. Van Vliet
Western blot w/subsequent sequencing Robert Phelps
Green-Fluorescent Protein Purification short course. Daniel Gonzalez
Green-Fluorescent Protein Recombinant DNA short course Daniel Gonzalez
Hoefer TKO 100 Mini-fluorometer Michael Cooley
EtBr toxicity Administrador de iihema.fmed.uba.ar
DNA quantitation. Administrador de iihema.fmed.uba.ar
6-His sequence lost during PCR Travis
RNA preparation Yoshiyuki Shibata
Hoefer TKO 100 Mini-fluorometer Biometreag
Preparative electrophoresis Uwe Kaerst
How to remove Commossie Brilliant Blue Uwe Kaerst
Tri-color FISH, suggestion needed H. Woo
Please help with transfection Peter Hagglov
6-His sequence lost during PCR Duncan Clark
Mac HPLC manager Nigel Kenward
A method to mark plasmids? Thomas
[Q] Labeled oligo gel purification alternatives? (Qiagen?) john brennand
Does 6-His inhibit protein's activity? Thomas
help - imaging Douglas Prasher
Very accurate determination of DNA-concentration Bo Servenius
Source for murine T-cell lines Dirk Seegert
construction of c-fos expression vector atdt31996
help - imaging john brennand
Texas red vs FITC Christopher Schultz
What is NTCB that cleaves proteins simon
enzyme for cloning? Mara Michelle Casar
RNA preparation yewks at teleview.com.sg
Marathon cDNA PCR Newswatcher
Do you have a lab web page? Christopher Schultz
help - imaging Richard P. Grant
Help me to find an author or his article please Gabriela Martinez Trevi o
How to remove Commossie Brilliant Blue Ron Tate
Chromosomal Gene deletions Linda Lambourne
Re- Tri-color FISH Brett Beitzel
Do you have a lab web page? Rafael Maldonado
bacterial two hybrid system ae at dna.bio.warwick.ac.uk
Protocol for GSH affinity matrix Curt Ashendel
DNA quantitation. david hanzel
Looking for Method to Purify Culture Cell Nuclei and Nuclear Membranes Samuel C. Blackman
IP3 prouction David Tak Yan Yu
Visit The First Fungal Genome Workshop HP Rolf A. Prade
Chromosomal Gene deletions Mikhail Alexeyev
Do you have a lab web page? Iain Wilson
Hoefer TKO 100 Mini-fluorometer James Graham
Mannose 6 phosphate receptor antibody Thillai Koothab
Ligations that are inhibited by adding insert Rafael Maldonado
Protein extraction from tissue Ed Castro
Quantifying mRNA on Northerns Ed Castro
HELP HELP HELP needed with RNA extraction Shehrebanoo Malik
RNA extractionmy email address Shehrebanoo Malik
Recombinant DNA methods workshops in California Mustafa Varoglu
prokaryotic "Chromosome"? Chi-Kuang Wen
phosphorylation software Joseph M. Lee
pCANTAB5E Csaba Jeney
Western blot w/subsequent sequencing Mick Partis
-20oC storage of bacterial stocks? Richard P. Grant
[Q]: nonradioactive RNase protection assay Dana Pozarkova
glucorticoid detection reagents LLewelyn Roderick
-20oC storage of bacterial stocks? julian catmull
Westerns, biotin-avidin and spurious bands LLewelyn Roderick
Westerns, biotin-avidin and spurious bands LLewelyn Roderick
Western blot w/subsequent sequencing Mick Partis
Help! Please help to locate a "Dip Miser" Robert Cowherd
Phycoerythrin stabilisation for microscopy? Julian F Dye
EtBr toxicity Kiley R. Prilliman
Nucleospin or Qiaprep spin buffers Nucleobond AX
bacterial two hybrid system James Graham
coating of microtiter plates with cell membranes? Volker Blaschke
indoleacrylic acid 74041.235
HTLV PCR Kits Ian A. York
bFGF receptor plasmid Barbara GILLON
bFGF receptor gene Barbara GILLON
Wilms' Tumour(tumor) cell lines Mr. D.M. Shaw
denaturing dna on southern blot j.r.stephen
mammalian expression vector User Name
bFGF receptor plasmid Mikhail Alexeyev
RT activity of Taq Bernard Murray
bacterial two hybrid system Mark Walberg
primer design software Walter L. Trudeau
4kb PCR product resists cloning M.E. Hudson
mammalian expression vector FirstName LastName
Q: Who invented PAAG holder with a cutout? Alexander Kraev
cosmid instability -Please help! Deborah Wilkinson
nORTHERN BLOT QUANTITATION Steve Griffiths
FISH and CGH need protocols Ingen at ingenlabs.com
Phycoerythrin stabilisation for microscopy? Chris Barry
Seeking plasmid vector pGB2 James Graham
Westerns, biotin-avidin and spurious bands Paul N Hengen
May EtBr affect sequencing? Paul N Hengen
Directionality of HIV Clones upyers
Do you have a lab web page? Paul N Hengen
Lysing of CHO cells for western blot?? Pramathesh S. Patel
cleaning up my probes Paul N Hengen
cleaning up my probes Paul N Hengen
library characterization? L. Bastian
pulse-field electrophoresis Leung Kwan Chi
PCR machine SRINIVAS MUMMIDI
HELP HELP HELP needed with RNA extraction Dimple Win
Overexpressed protein in inclusion bodies KHUANG at CHEMVX.TAMU.EDU
-20oC storage of bacterial stocks? Adrian.Philbey at SMTPGWY.AGRIC.NSW.GOV.AU
pulse-field electrophoresis WSchick at aol.com
Q: Who invented PAAG holder with a cutout? WSchick at aol.com
looking for antibodies Frank van Breukelen
coating of microtiter plates with cell membranes? Bernard Murray
Tri-color FISH, suggestion needed gabriele kerber
Nucleospin or Qiaprep spin buffers QIAGEN
random priming BERTHE thierry
Directionality of HIV Clones Olav Hungnes
RNA-labeling PC16 at
looking for antibodies Julian F Dye
Biotin-Riboprobes Joachim Kremerskothen
Primer design software Jim Hill
inhibition or inactivation of lysozyme ROBERT SAUNDERS
calcium measurement Sultan Kalifa
Source of macaloid Sultan Kalifa
-20oC storage of bacterial stocks? Michael Benedik
Elisa jms5 at Lehigh.EDU
commerical microsatellite prep? bio_hannan at emuvax.emich.edu
random priming KHUANG at CHEMVX.TAMU.EDU
Glycogen precipitation of DNA ? yewks at teleview.com.sg
Overexpressed protein in inclusion bodies john brennand
random priming Ziping Yang
Protein refolding help Shiverer
-20oC storage of bacterial stocks? Ned Mantei
Is an NLS essential for a co-IP of a nuclear protein? Mitarbeiterin Mmh
Pointer to methods FAQ list Paul N Hengen
Help locating a company J. Ibave
EtBr in partial RE digests Paul Digard
Glycogen precipitation of DNA ? Paul N Hengen
TKO miniflourometer (long) Philip L. Carl
Human Myb protein Shobha A. Seetharam
sequencing PCR-Products HIRANYA ROYCHOWDHURY
Can "Metaphor" agarose be re-used ? MSJSAR at leeds.ac.uk
Is an NLS essential for a co-IP of a nuclear protein? milstone
sequencing PCR-Products KHUANG at CHEMVX.TAMU.EDU
desperately looking for NGF receptor, PDGF receptor, and PKC-delta cDNA Myung Jong Kim
Position open Judit Monis
random priming Chi-kuang Wen
reg: radioimmuno assays xxx
HELP HELP HELP needed with RNA extraction neville
How to remove Commossie Brilliant Blue Achim Recktenwald
cell lines Martha Ogilvie
cell lines Martha Ogilvie
Help: Antibodies to DP-2 joestudent at mit.edu
Need anti-dopamine (anything but rabbit) Christopher Schultz
Have you heard of ImmunoTech or Immunonuclear Corp? Christopher Schultz
contaminating fibroblasts KRIS B
RIA service for biomedical research Pak Brian
Overexpressed protein in inclusion bodies KHUANG at CHEMVX.TAMU.EDU
Help! Please help to locate a "Dip Miser" vilimf01 at mcrcr6.med.nyu.edu
Spots on northern with oligo probes Hugues Lecomte
Anyone heard of Moravek Biochemicals roseng at colorado.edu
Directionality of HIV Clones Greg Tobin
Nucleospin or Qiaprep spin buffers David Micklem
HyperPAPER source wanted Mike Deyholos
primer design software Ed Castro
What is NTCB that cleaves proteins?? Charles Cobb
sequencing PCR-Products Huang Ruguo
random priming Farid jalali
Seeking plasmid vector pGB2 Peter Wang
glucorticoid detection reagents L. Bastian
Stabile transfection of Cos-7 olle vidal
cresol red dye Raja kota
Have you heard of ImmunoTech or Immunonuclear Corp? john brennand
cell lines john brennand
HyperPAPER source wanted john brennand
Help! Bacterial recombination in recA- cells Chris Boyd
Intracellular metal concentrations Richard Beck
Have you heard of ImmunoTech or Immunonuclear Corp? CHARLES PIAZZA
RNA preparation Paul Digard
contaminating fibroblasts Anne Exton Stone
Gel drawing program? Peter Russell
Westerns, biotin-avidin and spurious bands George W Chacko
coating of microtiter plates with cell membranes? Bernard Murray
Can "Metaphor" agarose be re-used ? STEVEN GERHARDT
-20oC storage of bacterial stocks? Michael Szardenings
cell lines Bob Sheridan
glucorticoid detection reagents Bernard Murray
PCR on imprinted genes? Peter McClive
phage lysate preparation insermet at univ-rennes1.fr
Is GAPDH an intronless gene Charles Dauphin
Westerns, biotin-avidin and spurious bands Paul N Hengen
Nucleospin or Qiaprep spin buffers Paul N Hengen
HELP!--- DNA Etraction from mushroom. z.h.lu-zh
RT activity of Taq orlando dominguez
Nasty looking buffer Laurence Tiley
reverse transcriptase question Brian A. Flannigan
Antibodies towards phosphorylated proteins Stressed Group
how do I transform a 60kb plasmid? John Dixon
non-radioactive probes John P. McGrath
Phycoerythrin stabilisation for microscopy? William Tivol
random priming Bernard Murray
Rat/mouse embryonic cDNA libraries, where? Ed Castro
cosmid library Ms Edith Erika Grimm
Primer design software Ed Castro
Uni-directional deletions Pamela Norton
how do I transform a 60kb plasmid? Mikhail Alexeyev
random priming Klaus Salger
RNA and PCR reaction KHUANG at CHEMVX.TAMU.EDU
Hoefer TKO 100 Mini-fluorometer Josee Ash
"Gently Resuspend" Brett Beitzel
Image analysis software David Tak Yan Yu
truncated beta-gal reporter JAne
truncated lacZ reporter Rodger
HELP: ES cell injection into blastocysts using C57BL/6 or F1-Hybrid C57BL/6xCBA Dr. Alexander J. Annala
biotin RNA Jillian Johnston
Inverse PCR of 4-10 kb Jill Kellogg
Glycogen co-ppt of DNA Jill Kellogg
PCR Problems! Please help! Holly West
contaminating fibroblasts Andria Lee
HPLC NTP purification?? Tom Wilson
"Gently Resuspend" Sailesh Surapureddi
"Gently Resuspend" Richard Daines
how do I transform a 60kb plasmid? Denis-Larose, Claude
Uni-directional deletions Dr Clinton A L Monfries
DNA Primers Steven Goldstein
anti-digoxin antibodies for BMB Genius system work in the BMB Genius system. I am mostly doing genomic (Arabidopsis) Southerns work in the BMB Genius system. I am mostly doing genomic (Arabidopsis) Southerns Mike Prigge
reverse transcriptase question Ned Mantei
"Gently Resuspend" Ned Mantei
rare mRNA species Hisatoshi Shida
Gel drawing program? Matthew P. Frosch, M.D., Ph.D.
Commercial source for ADH antibodies??? Martin E. Feder
S&S membranes Szabadkai Gyorgy
IMPORTANT - BIOSCI Fundraising Update! BIOSCI Administrator
phage lysate preparation john brennand
Is GAPDH an intronless gene David Winterbourne
Expression Pattern of beta-amyloid precursor Dr. P.A. de Zoysa
"Gently Resuspend" Chris Boyd
sequencing PCR-Products Jasper Saris
Information about vector pDCR *
S&S membranes Brian A. Flannigan
how do I transform a 60kb plasmid? Dima Klenchin
tmac william d. walsh
Antibodies towards phosphorylated proteins Jim Woodgett
non-radioactive probes Thibaud Le Mouel
MMLV-RT ab's? Dan Burgess
Mungo, Northern again Szabadkai Gyorgy
cosmid labelling plikat
Gel elution methods Dubear Kroening
cDNA of and Antibodies against alpha V/beta III Integrins N.N
cDNA of and Antibodies against alpha V/beta III Integrins J.Langner
Westerns,biotin-avidin and spurious bands Michael Lydan
[Q]: nonradioactive RNase protection assay Tracy Aquilla
Using a hot glue gun for sealing glass plates Paul N. Hengen
Primer Design Software sites!!! Ed Castro
Image analysis software milstone
Image analysis software milstone
Image analysis software milstone
Image analysis software milstone
Image analysis software milstone
"Gently Resuspend" Marcel Hoffmann
"Gently Resuspend" Marcel Hoffmann
"Gently Resuspend" Marcel Hoffmann
truncated lacZ reporter Bernard Murray
Gel elution methods Paul N. Hengen
Gel elution methods J. David Spafford
DNA Primers Tonja Burshek
Damn the Specificity! Brett Beitzel
HPLC NTP purification?? Bernard Murray
Fecal DNA Extraction Ronald Alexander
Fecal DNA Extraction Ronald Alexander
cleaning up my probes Jil Tardiff
RNA extraction: very small tissue sample Ed Castro
BRCA! and diagnostics, patents... varyc.mmcri at mmc.org
HELP! PCR PRIMER LOOP Tm POLASH
Stabile transfection of Cos-7 Ian A. York
Bio-Rad's Macro-Prep supports - any comments? Dima Klenchin
Storage of Antibodies Sidney B. Cambridge
Gel elution methods Farid jalali
Plasmid pBR326 Petra Kaiser
Stabile transfection of Cos-7 Dima Klenchin
Stabile transfection of Cos-7 Dima Klenchin
Storage of Antibodies Dima Klenchin
Multiple promotors in one gene Hiroshi Inoue
Multiple promotors in one gene Hiroshi Inoue
Multiple promotors in one gene Hiroshi Inoue
Multiple promotors in one gene Hiroshi Inoue
HELP!--- DNA Extraction from Mushroom z.h.lu-zh
Anti-phosphoserine antibodies Chuck van Beveren
Plasmid pBR326 Stefan Wieland
Northerns - nitrocellulose or nylon ?? Patrick HJ Falckh
Gel drawing program? Bob Obar
looking for antibodies Bob Obar
Storage of Antibodies Bob Obar
MJ Woes Tay Hou Ngee Agnes
Green Fluorescent protein Steven Tait
cleaning up my probes Tori Brophy
TALON resin users question Jose F Gutierrez Marcos
AP1-luciferase reporter vector dzistrer
mRNA extraction from Gram positive bacteria x4pg at unb.ca
HELP HELP HELP needed with RNA extraction UUCP II
HELP HELP HELP needed with RNA extraction UUCP II
HELP HELP HELP needed with RNA extraction UUCP II
HELP HELP HELP needed with RNA extraction
help with circular dichroism Alvin Dobbin
transient and stable transfection kits??? Shanthi Adimoolam
Gel drawing program? J. David Spafford
plasmid wanted... Rejean_Lapointe
Source of Celite-235? Shiao Wang
intron/exon map figures Susan B. McLeskey
HELP HELP HELP needed with RNA extraction Chi-kuang Wen
PCR getting smear Zahra Fayazi
PCR getting smear Zahra Fayazi
PCR getting smear Zahra Fayazi
PCR getting smear Zahra Fayazi
TA Cloning in Aust. Brendan Scott (brendans at pathology.su.oz.au)
MJ Woes Andrew Schrader
Multiple promotors in one gene gc
ThioFusion expression system KHUANG at CHEMVX.TAMU.EDU
GroE co-expression system KHUANG at CHEMVX.TAMU.EDU
question on BioRad Laemmli buffer Don Chen
transient and stable transfection kits??? A.Doherty
Bad plating efficiency Oliver Merk
Pharmacology Data Base. David Fogarty
Site-directed mutagenesis Eivind Johnsen
Address needed: Prof F Lacroute Botma Visser
G or C at 3' end of primer? Chris Skory
coating of microtiter plates with cell membranes? Keith
Plant CuZn SOD antibodies JL Montillet
G or C at 3' end of primer? Peter Kuhnert
question on BioRad Laemmli buffer Rejean_Lapointe
Directionality of HIV Clones Robin Beech
ThioFusion expression system David Michael Eisenmann
Subcloing PCR Products Jeff
HELP HELP HELP needed with RNA extraction Cornelius Krasel
EGFR Abs for westerns Mike Holbrook
cleaning up my probes Antonio Izzo
Plant CuZn SOD antibodies Mick Partis
HELP!--- DNA Extraction from Mushroom Antonio Izzo
Northerns - nitrocellulose or nylon ?? HIRANYA ROYCHOWDHURY
Plasmid pBR326 HIRANYA ROYCHOWDHURY
pCDNA3 Cloning Difficulties Kathy L
beta-galactosidase assays using microtitre plates Kerry Barne
G or C at 3' end of primer? Ed Castro
G or C at 3' end of primer? Ken Howe
pCDNA3 Cloning Difficulties Mikhail Alexeyev
Subcloing PCR Products Chi-kuang Wen
TKO miniflourometer (long) John Brunstein
Plasmid E. coli library. Rafael Maldonado
dye migration on PAG Nicholas Plummer
the RT/PCR standard that isn't Brian A. Flannigan
Site-directed mutagenesis N Ward
Damn the Specificity! L. Bastian
unwanted plasmid deletions...subhuman Luti Erbeznik
Subclone PCR product KHUANG at CHEMVX.TAMU.EDU
Can "Metaphor" agarose be re-used ? Rob Fialho
contaminating fibroblasts Kelly Ambler
Help with Diefenbakia and Yucca Plant, Pls edk at nortel.ca
dye migration on PAG HIRANYA ROYCHOWDHURY
Damn the Specificity Jill Kellogg
coating of microtiter plates with cell membranes? Mike Chin
rare mRNA species Hisatoshi Shida
intron/exon map figures Garry Myers
SEAP assay Phillip R Thompson
Subcloing PCR Products John Brennand
PCR Problems! Please help! John Brennand
Plasmid E. coli library. John Brennand
Can METAPHOR agarose be reused? - Answers MSJSAR at leeds.ac.uk
double promoter cloning vectors (SP6 and T7) Miss. V.M. Hodges
PCR getting smear orlando dominguez
reverse transcriptase question orlando dominguez
Is GAPDH an intronless gene Carrie Ris / Erwin Pauws
Northerns - nitrocellulose or nylon ?? Thibaud Le Mouel
Subcloing PCR Products Harry J. Witchel
HELP: Retroviral packaging cell lines Thomas Mohr
Help with Diefenbakia and Yucca Plant, Pls Monique Reed
Ionic Strength? JG. Head
double promoter cloning vectors (SP6 and T7) I.S.Wood
prok disc group BADIDION at aol.com
email address BADIDION at aol.com
Anti-phosphoserine antibodies Uwe Hobohm
Anti-phosphoserine antibodies Uwe Hobohm
Anti-phosphoserine antibodies Uwe Hobohm
(none) Francisco G da Nobrega ib - bio 7588
Multiple promotors in one gene Tracy Aquilla
PCR getting smear Landry Pierre-Alexandre
pDCR Map ? *
how many bases does an RE cover? bajenkin at swansea.ac.uk
transient and stable transfection kits??? Simon Q.J. RICE
Site-directed mutagenesis Simon Q.J. RICE
PCR Problems! Please help! Christopher T. Cole
TKO Fluorometer Mayumi Yagi
biotin RNA William_P_Prichett
Looking for antibodies Chris Siow
Disposable NMR tubes Brad Riesenberger
Immunonuclear Corp JLCruise
2nd reminder Kalyanaraman
Can "Metaphor" agarose be re-used ? Pat Dunn
glucorticoid detection reagents Heinz-Juergen Schaefers
2nd reminder HIRANYA ROYCHOWDHURY
Primer specificity and olig Brett Beitzel
Recircularize blunt end plasmid Elizabeth Rosenberg
luminol reagents Brian McCauley
Subcloing PCR Products Minsoo Yoon
TALON resin users question Tom Vink
T4-RNA ligase Chr. Wagenknecht
DNA staining (non toxic) Dan
dissolving soybean lectin Otso Lindy
HELP: Retroviral packaging cell lines John Brennand
What is the target of staurosporine ? Stephane Ory
DNA Sequencers Nancy Stokes
Subcloing PCR Products JG. Head
Clontech epidermal cDNA libraries Nick Jacobsen
GCK (was "Re: Gel drawing program?") Peter Wang
[Q]: E.coli for high GC-content? malbrech at vulpius-eth.rz.uni-frankfurt.de
Agrobacterium plasmid preps Jill Kellogg
tets Els Van Geldre
Qiagen Web Site? Richard Daines
cDNA library size Els Van Geldre
Looking for MALDI Mass Spec service Randall Willis
Wretched dinucleotide repeats Steve Griffiths
mouse derived cells equivalent to COS cells Takahiko Ito
What is the target of staurosporine ? Curt Ashendel
commerical microsatellite prep? mullen at SMALL3.jsei.ucla.edu
? Counting Cells using a desktop scanner ? Wolfgang Schechinger
Wretched dinucleotide repeats mullen at SMALL3.jsei.ucla.edu
H E L P ! ! ppadula at IMPSAT1.COM.AR
Good Protein Expression System... Suggestions? Michael Oda
cDNA library size Chi-kuang Wen
Recircularize blunt end plasmid Elizabeth Rosenberg
cleaning up my probes Dima Klenchin
double promoter cloning vectors (SP6 and T7) ijiwaru at wheel.dcn.davis.ca.us
Have you seen a software program called GeneStat? Michael Arthur Wall
T4-RNA ligase ijiwaru at wheel.dcn.davis.ca.us
Good Protein Expression System... Suggestions? ijiwaru at wheel.dcn.davis.ca.us
cleaning up my probes HIRANYA ROYCHOWDHURY
anti-TGFbeta antibodies, etc. siow at unixg.ubc.ca
TKO Fluorometer mullen at SMALL3.jsei.ucla.edu
Genomic labelling in-situ of plant DNA Chromosome Terror
Damn the Specificity! Chromosome Terror
None Roberto Cappai
Phenol Stories Biometreag
Effect of acetone on DNA for PCR? Robert Clark
cleaning up my probes Lo Wing Ip Anthony
Effect of acetone on DNA for PCR? Chi-Kuang Wen
cleaning up my probes Thibaud Le Mouel
BAC - Fosmid Vectors Mick Jones
Ionic Strength? simon
mucosal immunization LPS BJ Appelmelk
red X gal Cheah Kathryn Song Eng
In vitro immunization of B-cells? Geir Johannessen
Looking for MALDI Mass Spec service John E. Fox
Northerns - nitrocellulose or nylon ?? Yves Poumay
luminol reagents Dr E. Buxbaum
Phenol Stories John Brennand
Anti-sense RNA Mozart de Azevedo Marins
G or C at 3' end of primer? Tom Dougherty
HELP HELP cDNA library Els Van Geldre
pCDNA3 Cloning Difficulties Helmut
cowpea nodule library pluan
Uni-directional deletions Blanca Lupiani
RNAse protection assays Dr. T.F. King
red X gal Raymond Dalgleish
HELP!-Sequencing GAL4 binding sites Verdin Lab
"gently resuspend" Denni Schnapp
Gedankenexperiment Sidney B. Cambridge
Looking for antibodies Claire Ruzicka
P. ovale genomic DNA library LI, TANG
StratagenQuikChange Tori
DNA Sequencers Steven Goldberg
Vancomycin resistance Johan Burger
Anti-sense RNA Martin G. Hulsey
cleaning up my probes Matthew J. F. Waterman
DNA Sequencers Phillip SanMiguel
Anyone using "the hook"? jovermeyer at shrsys.hslc.org
Phenol Stories HIRANYA ROYCHOWDHURY
Looking for Fau 1. mc97 at PO-Box.McGill.CA
What is the target of staurosporine ? Jim Woodgett
HELP!-Sequencing GAL4 binding sites Sailesh Surapureddi
QUESTIONS:LIVE/DEAD BACLIGHT BACTERIA VIABILITY KIT L-7007. Recently we have tried to use the reagent (Lot:0251-2). But, 1. There was no red dye detected after incubating the reagent with bacteria containing bacteria-killing compound. Our fluorescence microscope filter is FITC, Ex:480 +/-40; Em: 535 +/-50.These data are very close to the filter recommended by the Company which was said good for detect both green (stained alive bacteria) and red (dead bacteria) color. 2. The background of the reagent (green color) is very high. If you have used this reagent, or you have any explanation to the questions, please help me. Thanks. My E-mail: ytang@uci.edu. Nannette Yount
(no subject) Alexander Vinitsky
2hybrid in Bacteria? L. Bastian
Looking for Fau 1. David Micklem
[Q] Labeled oligo gel purification alternatives? (Qiagen?) martin LEACH
Help : detection of mononucleotide on TLC swine at plaza.snu.ac.kr
QUESTIONS:LIVE/DEAD BACLIGHT BACTERIA VIABILITY KIT L-7007.[snip] Dima Klenchin
Damn the Specificity! Enrique Jose Labadan Frio - SCBC - 3421101
Sequencing large DNA by transposon Bradley Turner
DNA Sequencers Robert G. Hamilton
(none) postmast at iihema.fmed.uba.ar

Last message date: Fri May 31 22:14:56 EST 1996
Archived on: Sun Mar 6 21:04:36 EST 2005

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