Can ssDNA transform competent cell ?
plw at mrc-lmb.cam.ac.uk
Fri Nov 1 14:24:21 EST 1996
wliu at receptor.pharm.uwa.edu.au wrote:
> Does anybody know that if the purified ssDNA (isolated from bacteria
> containing phagemid after infection of helper phage RO408) can
> transform competent cells and recover the dsDNA replication mode?
Yes it should. Transformation with phagemid ssDNA is analogous to
infection with phagemid that has been packaged with a helper phage; once
in the bacteria, the phage ori in single-stranded form directs priming
and synthesis of the complementary strand by host enzymes to give dsDNA.
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