Inclusion of restriction sites in PCR primers

Alex Merz merza at
Sun Nov 3 00:01:05 EST 1996

In article <E06wHC.G4q at>,
Harry Witchel  <Harry.Witchel at Bristol.Ac.Uk> wrote:
>2)  Put four bases 5' to the cutting site.  You have already done this.  
>I tend to use GAGA, but any combination which does not use 4 identical 
>bases should be OK.

The recommendations in the NEB catalog are fine, in my limited 
experience. If the catalog says you don't need an extra base, 
you probably don't.


More information about the Methods mailing list