Inclusion of restriction sites in PCR primers
merza at ohsu.edu
Sun Nov 3 00:01:05 EST 1996
In article <E06wHC.G4q at fsa.bris.ac.uk>,
Harry Witchel <Harry.Witchel at Bristol.Ac.Uk> wrote:
>2) Put four bases 5' to the cutting site. You have already done this.
>I tend to use GAGA, but any combination which does not use 4 identical
>bases should be OK.
The recommendations in the NEB catalog are fine, in my limited
experience. If the catalog says you don't need an extra base,
you probably don't.
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