Inclusion of restriction sites in PCR primers

Alex Merz merza at ohsu.edu
Sun Nov 3 00:01:05 EST 1996


In article <E06wHC.G4q at fsa.bris.ac.uk>,
Harry Witchel  <Harry.Witchel at Bristol.Ac.Uk> wrote:
>2)  Put four bases 5' to the cutting site.  You have already done this.  
>I tend to use GAGA, but any combination which does not use 4 identical 
>bases should be OK.

The recommendations in the NEB catalog are fine, in my limited 
experience. If the catalog says you don't need an extra base, 
you probably don't.

-Alexey






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