Inclusion of restriction sites ...ERRATUM

Ashok Aiyar aiyar at ebv.oncology.wisc.edu
Sun Nov 3 23:19:36 EST 1996


On Mon, 04 Nov 1996 02:15:21 +0000, Andrei Popov <andrei.popov at bbsrc.ac.uk> wrote:
>
>That must have been a REAL miracle!
>If you have:
>
>    aattcNNNNNNNNNgaatt   +  aattcNNNNNNNNNgaatt
>    ttaagnnnnnnnnncttaa   +  ttaagnnnnnnnnncttaa
>
>plus phosphatase plus T4 ligase and you got a digestable EcoRI site?!
>star activity or what?

Why is this so surprising?  Self-ligated products cloned perfectly
into the NdeI site of pCDNA3, which I believe is what I wrote.

You do realize that the *kinased* (not phosphatased) self-ligated 
product has AseI sites at the junctions, right?

Ashok




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