Inclusion of restriction sites ...ERRATUM

[David Micklem]

In article <slrn57qrmo.4p7.aiyar at ebv.oncology.wisc.edu>,
aiyar at ebv.oncology.wisc.edu wrote:

>On Mon, 04 Nov 1996 02:15:21 +0000, Andrei Popov
<andrei.popov at bbsrc.ac.uk> wrote:
>>
>>That must have been a REAL miracle!
>>If you have:
>>
>>    aattcNNNNNNNNNgaatt   +  aattcNNNNNNNNNgaatt
>>    ttaagnnnnnnnnncttaa   +  ttaagnnnnnnnnncttaa
>>
>>plus phosphatase plus T4 ligase and you got a digestable EcoRI site?!
>>star activity or what?
>
>Why is this so surprising?  Self-ligated products cloned perfectly
>into the NdeI site of pCDNA3, which I believe is what I wrote.


Funny! On _my_ newsreader it came out:

>P.S. The PCR product I was attempting to clone, did clone when
>     self-ligated (after 5' phosphorylation) followed by digestion
>     with EcoRI.

Maybe we're talking about different messages here?

David

_____________________________________________________________
D.R.Micklem,
Wellcome/CRC Institute,       Time flies like an arrow...
Tennis Court Road,              
Cambridge CB2 1QR             Fruit flies like a banana.
UK                             
Tel: [+44] (0)1223 334129     Email:drm21 at mole.bio.cam.ac.uk
Fax: [+44] (0)1223 334089               
_____________________________________________________________