Inclusion of restriction sites ...ERRATUM

David Micklem drm21 at mole.bio.cam.ac.uk
Mon Nov 4 06:26:51 EST 1996


In article <slrn57qrmo.4p7.aiyar at ebv.oncology.wisc.edu>,
aiyar at ebv.oncology.wisc.edu wrote:

>On Mon, 04 Nov 1996 02:15:21 +0000, Andrei Popov
<andrei.popov at bbsrc.ac.uk> wrote:
>>
>>That must have been a REAL miracle!
>>If you have:
>>
>>    aattcNNNNNNNNNgaatt   +  aattcNNNNNNNNNgaatt
>>    ttaagnnnnnnnnncttaa   +  ttaagnnnnnnnnncttaa
>>
>>plus phosphatase plus T4 ligase and you got a digestable EcoRI site?!
>>star activity or what?
>
>Why is this so surprising?  Self-ligated products cloned perfectly
>into the NdeI site of pCDNA3, which I believe is what I wrote.


Funny! On _my_ newsreader it came out:

>P.S. The PCR product I was attempting to clone, did clone when
>     self-ligated (after 5' phosphorylation) followed by digestion
>     with EcoRI.

Maybe we're talking about different messages here?

David

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D.R.Micklem,
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