Inclusion of restriction sites ...ERRATUM
drm21 at mole.bio.cam.ac.uk
Mon Nov 4 06:26:51 EST 1996
In article <slrn57qrmo.4p7.aiyar at ebv.oncology.wisc.edu>,
aiyar at ebv.oncology.wisc.edu wrote:
>On Mon, 04 Nov 1996 02:15:21 +0000, Andrei Popov
<andrei.popov at bbsrc.ac.uk> wrote:
>>That must have been a REAL miracle!
>>If you have:
>> aattcNNNNNNNNNgaatt + aattcNNNNNNNNNgaatt
>> ttaagnnnnnnnnncttaa + ttaagnnnnnnnnncttaa
>>plus phosphatase plus T4 ligase and you got a digestable EcoRI site?!
>>star activity or what?
>Why is this so surprising? Self-ligated products cloned perfectly
>into the NdeI site of pCDNA3, which I believe is what I wrote.
Funny! On _my_ newsreader it came out:
>P.S. The PCR product I was attempting to clone, did clone when
> self-ligated (after 5' phosphorylation) followed by digestion
> with EcoRI.
Maybe we're talking about different messages here?
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