ECL reagents

Dr E. Buxbaum EB15 at
Mon Nov 4 06:06:21 EST 1996

"A. Piccin" <AP12 at> wrote:

>Does anybody know the recipes for a reliable luminescent detection reagent?

I spend some time fooling around with this. The best results so far I 
obtained with the following mixture:
40 ul 100 mM (17.6 mg/ml) 4-Methylumbelliferone in DMSO
75 ul 10 mM (1.77 mg/ml) Luminol in DMSO
6.15 mg Sodium perborate (or 2.8 ul 30% Hydrogen peroxide)
Aq. bidest. ad 10 ml

Works reliably and reproducible, but is slightly less sensitive than 
comercial stuff. Quite cheap, though. 

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