protein fractionation ?

[Svend Kjaer]

Hi all !
I need to fractionate cytosolic proteins in a manner that will 
lower the complexity of a cytosolic protein preparation. I don't care much
about the criteria used for the fractionation as long as it can be applied
to small amounts of protein with a relatively high degree of reproducibility
between different cell types.
Any input appreciated

Thanx

Svend Kjaer
Ph.D. student
Institute of Molecular and Structural Biology
University of Aarhus, Denmark