mRNA Transcript Quantitation Method [Q]

Øivind Enger oivind.enger at im.uib.no
Mon Nov 4 11:00:10 EST 1996


Hi Michal
If your mRNA is pretty clean, you could try SybrGreenII (from Molecular
Probes, Eugene OR). It is very sensitive, but I guess you would need to
have all other nucleic acids removed prior to measurement.
good luck

-oivind


Michael Oda <mnoda at lipovx.lbl.gov> wrote in article
<mnoda-0111961246560001 at 207.126.101.77>...
> Hi,
>      We are looking for a means to quantitate a transcript from
> mouse liver. Under the conditions of the experiment we expect a
> percent change in levels of transcript per mass of tissue in
> the 5% to 20%  range. 



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