Cell Lysing Detergent
un691cs at genius.embnet.dkfz-heidelberg.de
Tue Nov 5 09:50:14 EST 1996
In article <327E2B67.65C9 at bms.com>, watson_j at bms.com says...
>Rick Bright wrote:
>> Also, is there a particular reason why one would use Nonidet P-40 over
>> SDS or Triton X-100 for lysing blood cells? Are there particular
>> applications where one agent is better than others, or are do they serve
>> the purpose equally well for all applications?
>We used to use NP-40 as the lysis agent when we wanted to dissolve the plasma
>membrane but leave nuclear membrane intact. A couple of examples were when
>making total cytoplasmic RNA and when preparing nuclear extracts for gel
>mobility shift assay.
I thought most NP-40 and Tween-20 and obscure substances like that have indeed
been replaced by Triton X-100. I remember wanting to order NP-40 a few years
back, as I added this stuff to my PCRs: a sales representative of the company that
actually has patents on these things (don't ask me the name, I forgot) told me NP-40
and Tween are now replaced by Triton X-100.
and it works just as well.
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