chloramphenicol resin

Uttam Rajbhandary bhandary at wccf.mit.edu
Wed Nov 6 14:39:00 EST 1996


I am trying to use a chloramphenicol resin from Sigma to retrieve CAT reporter 
protein made in mammalian cells during transfection.  I'm having a problem 
with elution; can't get it back. I'm using a 1M NaCl/5mM CAM elution; 0.3M 
NaCl as recommended by Sigma will not work for TN9 derived CAT (Type I, 
original reference notes this; Type I needs high salt to recover).
 Has anyone successfully used this resin to 
do this from mammalian cells?



More information about the Methods mailing list