bhandary at wccf.mit.edu
Wed Nov 6 14:39:00 EST 1996
I am trying to use a chloramphenicol resin from Sigma to retrieve CAT reporter
protein made in mammalian cells during transfection. I'm having a problem
with elution; can't get it back. I'm using a 1M NaCl/5mM CAM elution; 0.3M
NaCl as recommended by Sigma will not work for TN9 derived CAT (Type I,
original reference notes this; Type I needs high salt to recover).
Has anyone successfully used this resin to
do this from mammalian cells?
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