WANTED: Marking Pen for Autorads

Chromosome Terror abrdlher at reading.ac.uk
Wed Nov 6 14:40:32 EST 1996


> > hello All,
> > 
> > Can anyone suggest a marking pen to mark blots?  I'm looking for a 
> > luminescent-type pen which will 'burn' a mark into the autorad so it can 
> > be aligned with a blot or lift.  In the past I have used labeled ink 
> > (35S) but we no longer use this isotope regularly plus its not very safe 
> > (although it does work prety well).  I have also tried lumninesent nail 
> > polish but this sticks to the autorads which is pretty messy.  I have 
> > seen some rediculously expensive 'stickers' marketed by Stratagene and 
> > Sigma, haven't tried them because of price.
> > 
> > Any suggestions for 'home grown' markers or a commercailly available marker?
> > 
> > Thanks in advance,
> > 
> > Paul Bucciaglia

I use a regular wax pencil. I write on the membrane with it. I use the
luminiscent ECL kit from Amersham. Whatever I write on the membrane is
visible afterwards in the developed film.

Nacho de las Heras
Botany Dept.
University of Reading
Reading RG6 2AS
UK





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