Reasons for PCR failure....?
demed at vms.ucc.okstate.edu.
Wed Nov 6 13:26:21 EST 1996
We had some problems for a long time until one of my graduate students
started looking at the storeage buffer of Taq pol. There is a big
difference in the storeage buffers. Some storeage buffers have Triton
X-100 and others have nonidet-P40 and Tween 20. Our best results have
been with those buffers with nonidet and tween which by the way I
believe PE Taq is stored in this type of buffer. Check your buffers;
like you we tried everything-so we thought.
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