Immunoblot semi-quantitative at best?
jeffb at sccwrp.org
Wed Nov 6 11:54:33 EST 1996
I have read that Western blotting is best used to determine the presence
or lack of presence of a protein, and should not be used as a
quantitative tool. The reasoning is that the antibody-antigen
relationship and antibody-antibody interactions are not a one-to-one
ratio. Also, homologous antibodies are not always available.
Is this the consensus? Is ELISA a better quantitative tool?
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