Isolating RNA without using DNase??
coulier at infobiogen.fr
Fri Nov 8 02:46:05 EST 1996
On 8 Nov 1996, Qunfeng Dong wrote:
> Hi, there:
> When I read the "current protocols in molecular biology", I am curious why
> those RNA isolation procedures don't need to use DNase in any step, how is
> DNA removed during the isolation??
> Qunfeng Dong
> qfdong at iastate.edu
Some methods use density gradient (in CsCl, for example) to separate DNA
from RNA. RNA is denser, and can be pelleted throgh a cushion, DNA
remaining in the upper portion of the tube.
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