Isolating RNA without using DNase??

Francois Coulier coulier at infobiogen.fr
Fri Nov 8 02:46:05 EST 1996


On 8 Nov 1996, Qunfeng Dong wrote:

> 
> 
> 
> Hi, there:
> 
>   When I read the "current protocols in molecular biology", I am curious why
> those RNA isolation procedures don't need to use DNase in any step, how is
> DNA removed during the isolation??
> 
> Qunfeng
> -- 
> Qunfeng Dong
> qfdong at iastate.edu
> 
> 
Some methods use density gradient (in CsCl, for example) to separate DNA
from RNA. RNA is denser, and can be pelleted throgh a cushion, DNA
remaining in the upper portion of the tube.
 Francois Coulier
INSERM Unite 119
27 bd Lei Roure
13009 Marseille
France

tel 33 (0) 4 91 75 84 11
fax 33 (0) 4 91 26 03 64)
email: coulier at infobiogen.fr




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