rTth reverse transcriptase kit

Martin Offterdinger a8803349 at unet.univie.ac.at
Thu Nov 7 05:10:02 EST 1996


In article <55r8f6$gdr at bubba.NMSU.Edu>, zyu at nmsu.edu (Z. YU) says:
>
>Does anyone have any experience with the rTth reverse
>transcriptase kit made by Perkin Elmer? I tried it, but
>I could not get it work, I means with the positive control
>included in the kit. Any info will be appreciated.
 
Hi
I have used a similar kit("XL RNA PCR") which uses rTTh Polymerase to do 
RT-Pcr . I have used it successfully to clone a 2kB fragment, without any 
mistake. I have used specific priming according to the Perkin Elmer
instructions.But later on it did not work that well.

Martin



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