Suggestions for expressing two proteins in E. coli

Boris Steipe steipe at LMB.uni-muenchen.de
Fri Nov 8 08:43:36 EST 1996


Steven Goldberg wrote:
> 
> I am looking for ideas on how to simulataneously express two recombinant
> proteins in E. coli.

The classic case is the simultaneous expression of heavy- and
light-chain domains of immunoglobulins. These are best translated off a
bicistronic message, for a number of reasons. A litsearch for "Arne
Skerra" will give you a wealth of ideas.

Good luck,
Boris

-- 
     +---Dr. Boris Steipe------------<steipe at LMB.uni-muenchen.de>---+
     |   Genzentrum                                                 |
     |   Feodor-Lynen Str. 25            Tel +49 (0)89 74017-417    |
     |   81377 Muenchen, Germany         Fax               - 448    |
     +---<http://www.LMB.uni-muenchen.de/users/steipe/boris.html>---+



More information about the Methods mailing list