Suggestions for expressing two proteins in E. coli

Boris Steipe steipe at
Fri Nov 8 08:43:36 EST 1996

Steven Goldberg wrote:
> I am looking for ideas on how to simulataneously express two recombinant
> proteins in E. coli.

The classic case is the simultaneous expression of heavy- and
light-chain domains of immunoglobulins. These are best translated off a
bicistronic message, for a number of reasons. A litsearch for "Arne
Skerra" will give you a wealth of ideas.

Good luck,

     +---Dr. Boris Steipe------------<steipe at>---+
     |   Genzentrum                                                 |
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