Immunoblot semi-quantitative at best?
mick.partis at hri.ac.uk
Fri Nov 8 07:08:26 EST 1996
Jeff Brown <jeffb at sccwrp.org> wrote:
>I have read that Western blotting is best used to determine the presence
>or lack of presence of a protein, and should not be used as a
>quantitative tool. The reasoning is that the antibody-antigen
>relationship and antibody-antibody interactions are not a one-to-one
>ratio. Also, homologous antibodies are not always available.
>Is this the consensus? Is ELISA a better quantitative tool?
You might also add the problem of accurately quantitating the amount of
product in western blots or dot-blots when using chromogenic substrates,
although the hardware and software currently available goes some way
towards addressing this problem.
mick.partis at hri.ac.uk Horticulture Research International
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