attachment HEK-293 cells

jstrassw at OPAL.TUFTS.EDU jstrassw at OPAL.TUFTS.EDU
Sat Nov 9 15:08:02 EST 1996

I asked a similar question 6 months ago.  The most useful rtesponse wat 
that after transfection with CaP04, DONT wash with PBS/EDTA (which we 
normally do to remove the Ca++) but insetad rinse gently with just PBS.

That helped us a bit.

Good luck!

John Strasswimmer, MD,PhD Candidate    | Phone (617) 636 8396
Tufts - New England Medical Center     | Fax   (617) 636 6190
Box 166                                | Email:  jstrassw at
Boston, MA 02111 USA                   |                              
     *** Co-Author of "HyperBug" Microbiology Teaching Software ***
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