attachment HEK-293 cells
jstrassw at OPAL.TUFTS.EDU
jstrassw at OPAL.TUFTS.EDU
Sat Nov 9 15:08:02 EST 1996
Hello!
I asked a similar question 6 months ago. The most useful rtesponse wat
that after transfection with CaP04, DONT wash with PBS/EDTA (which we
normally do to remove the Ca++) but insetad rinse gently with just PBS.
That helped us a bit.
Good luck!
John
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John Strasswimmer, MD,PhD Candidate | Phone (617) 636 8396
Tufts - New England Medical Center | Fax (617) 636 6190
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Boston, MA 02111 USA |
HTTP://WWW.healthsci.tufts.edu/microbiology/strass/JSpage.HTML
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