source of wheat germ please

Guido Brink gub at worldonline.nl
Sun Nov 10 17:53:44 EST 1996


Hi,

You can get it at Promega (they are also on the Web). They also have complete IVTT
(In Vitro Transcription Translation)-kits. You can also buy these at Amersham and Boehringer Manheim.

I use these systems a lot for IVTT of a 2kb PCR-product with T7-promoter and kozak sequence 
incoperated. Using Reticulocyte lysate and 35S-Methionine we get super-protein. So questions are 
welcome.

Guido Brink
Family Cancer Clinic
The Netherlands Cancer Institute
Amsterdam



More information about the Methods mailing list