How to purify DNA from PAGE?

Louis Alarcon lha at med.pitt.edu
Mon Nov 11 16:23:40 EST 1996


In article <567nau$mos at info.service.rug.nl>, g.j.e.j.hooiveld at farm.rug.nl
(Guido Hooiveld) wrote:

> Does anybody know some literature references or protocols concerning the 
> purification of DNA from acrylamide gels? Is for instance a method like 
> freeze-sqeeze for agarose gels also usable for PAGE?
> Guido

We use the "crush and soak" method described in Maniatis 6.46 with
excellent results.

Louis Alarcon, MD
University of Pittsburgh
Department of Surgery



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