How to purify DNA from PAGE?
Rover at livenet.net
Mon Nov 11 20:58:33 EST 1996
g.j.e.j.hooiveld at farm.rug.nl (Guido Hooiveld) wrote:
>Does anybody know some literature references or protocols concerning the
>purification of DNA from acrylamide gels? Is for instance a method like
>freeze-sqeeze for agarose gels also usable for PAGE?
>Thanks for any help!
We have tried just about every method and still use electro-elution
into dialysis bags as our favorite method. Simple to set up once you
have made a batch of of prepared dialysis tubing (See Maniatis) and
yields are 60-70%.
SWB at Di1.evms.edu
Molecular Biology Laboratory
The Diabetes Research Institutes
Eastern Virginia Medical School
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