How to purify DNA from PAGE?

Rover Rover at
Mon Nov 11 20:58:33 EST 1996

g.j.e.j.hooiveld at (Guido Hooiveld) wrote:


>Does anybody know some literature references or protocols concerning the 
>purification of DNA from acrylamide gels? Is for instance a method like 
>freeze-sqeeze for agarose gels also usable for PAGE?

>Thanks for any help!

We have tried just about every method and still use electro-elution
into dialysis bags as our favorite method. Simple to set up once you
have made a batch of of prepared dialysis tubing (See Maniatis) and
yields are 60-70%. 


SWB at
Molecular Biology Laboratory
The Diabetes Research Institutes
Eastern Virginia Medical School

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