BL21(DE3) pLYSe/s competent cells

ashaw5 at ashaw5 at
Wed Nov 13 01:40:12 EST 1996

Who cares?  We freeze our BL21/lysS homemade competents and yes the
efficiency is poor.  (Now I think I know the reason).  But you are
generally transferring a plasmid into BL21 from another bug and so you
don't need very efficient bugs.  100ng should easily give you enough
colonies even if your efficiency is 10-3.

Andrey Shaw

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