DD-PCR

shou Jiang shouj at NIC.BMI.AC.CN
Tue Nov 12 09:51:56 EST 1996


Dear Dr. Peter,

Thank you very much, please send the publication, your company catelog,
and copy of proceeding ofrecent symposium on ddpcr in CSH, to 

SHOU JIANG, PHD

Dept of Biochemistry
Inst of Radiation Medicine
Beijing 100850
Chna
Shouj at nic.bmi.ac.cn\


Thanks a lot.


Shou Jiang

----------
> From: WSchick at aol.com
> To: methods at net.bio.net; wudc at nic.bmi.ac.cn
> Cc: displaysystemsbiotech <dissyst at primenet.com>
> Subject: Fwd: DD-PCR
> Date: Tuesday, November 12, 1996 9:31 PM
> 
> In a message dated 96-11-11 11:54:44 EST, dissyst at primenet.com (Display
> Systems Biotech) writes:
> 
>  Forwarded message:
>  From:	wudc at nic.bmi.ac.cn
>  
>  Dear everyone
>  
>  The ones who have experince doing differential display,I want your
help!
>  
>  I used 0.2 micrograms total RNA to reverse transcription,and one-tenth
>  of the cDNA was used in DDPCR,but I got very faint bands,I think the
>  reason may be too little cDNA was used in the reaction.so I want to
>  know,how much total RNA is used to do reverse-transciption ,and how
much
>  cDNA is used to do DDPCR in your reaction?
>  If you have some tips or skills ,let me know.Thank you very much!
>  
>  
>  Answer,
>  
>  The amount of RNA and following cDNA synthesis is extremly important in
>  DDRT-PCR analysis.
>  I have now been in the DD-PCR world since the beginning back in 92. 
>  If you want to read more about our protocols please read some of
>  the most important publications in this field, if you want we can sent
>  copies.  In our DD kits all the parameter are completely optimized. 
>  
>  Best Regards
>  
>  Peter Warthoe, 
>  Display Systems Biotech   http://www.displaysystems.com
>  
>  Some method papers:
>  (please start with the chapter in the new Cold Spring Harbor book page
>  421):
>  
>  Bauer, D., Warthoe, P., and Strauss, M. Identification of
differentially
>  expressed mRNA species by an improved display technique (DDRT-PCR)
>  (1993). NAR 21: 4272-4280
>  
>  Warthoe, P., et al. Detection and identification of expressed genes by
>  differential display (1995).  PCR Primers: A Laboratory Manual. Cold
>  Spring Harbor Laboratory Press.
>  
>  Bauer, D.,  Warthoe, P., Liang, P., Averboukh, L., Rohrwild, M.,
Muller,
>  H., Strauss, M., and Pardee, AB. Analysis of altered gene expression by
>  differential display (1995). Methods-Enzymol. 254: 304-321 
>  
>  Rohde, M., Warthoe, P., Gjetting, T., Lukas, J., Bartek, J., and
>  Strauss, M. The retinoblstoma protein modulates expression of genes
>  coding for diverse classes of proteins including components of the
>  extracellular matrix (1996). Oncogene 12: 2393 - 2401.
>   >>
> 
> 
> ---------------------
> Forwarded message:
> From:	dissyst at primenet.com (Display Systems Biotech)
> To:	WSchick at aol.com
> Date: 96-11-11 11:54:44 EST
> 
> Dear Walt,
> 
> The complete address - Answer to the one asking about DD-PCR 
> 
> Forwarded message:
> From:	wudc at nic.bmi.ac.cn
> Sender:	daemon at net.bio.net
> Reply-to:	wudc at nic.bmi.ac.cn
> To:	methods at net.bio.net
> Date: 96-11-06 10:55:58 EST
> 
> Dear everyone
> 
> The ones who have experince doing differential display,I want your help!
> 
> I used 0.2 micrograms total RNA to reverse transcription,and one-tenth
> of the cDNA was used in DDPCR,but I got very faint bands,I think the
> reason may be too little cDNA was used in the reaction.so I want to
> know,how much total RNA is used to do reverse-transciption ,and how much
> cDNA is used to do DDPCR in your reaction?
> If you have some tips or skills ,let me know.Thank you very much!
> 
> 
> Answer,
> 
> The amount of RNA and following cDNA synthesis is extremly important in
> DDRT-PCR analysis.
> I have now been in the DD-PCR world since the beginning back in 92. 
> If you want to read more about our protocols please read some of
> the most important publications in this field, if you want we can sent
> copies.  In our DD kits all the parameter are completely optimized. 
> 
> Best Regards
> 
> Peter Warthoe, 
> Display Systems Biotech   http://www.displaysystems.com
> 
> Some method papers:
> (please start with the chapter in the new Cold Spring Harbor book page
> 421):
> 
> Bauer, D., Warthoe, P., and Strauss, M. Identification of differentially
> expressed mRNA species by an improved display technique (DDRT-PCR)
> (1993). NAR 21: 4272-4280
> 
> Warthoe, P., et al. Detection and identification of expressed genes by
> differential display (1995).  PCR Primers: A Laboratory Manual. Cold
> Spring Harbor Laboratory Press.
> 
> Bauer, D.,  Warthoe, P., Liang, P., Averboukh, L., Rohrwild, M., Muller,
> H., Strauss, M., and Pardee, AB. Analysis of altered gene expression by
> differential display (1995). Methods-Enzymol. 254: 304-321 
> 
> Rohde, M., Warthoe, P., Gjetting, T., Lukas, J., Bartek, J., and
> Strauss, M. The retinoblstoma protein modulates expression of genes
> coding for diverse classes of proteins including components of the
> extracellular matrix (1996). Oncogene 12: 2393 - 2401.
> 
> 



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