Fusion protein ativity

KHUANG at CHEMVX.TAMU.EDU KHUANG at CHEMVX.TAMU.EDU
Thu Nov 14 18:04:08 EST 1996


Hi, there,
I am using GST and thioredoxin fusion to express a few plant protein, the
molecular weight are more than 60 KDa. I tried four, three are activity, one
did not show activity, but when the protein was released from fusion protein, 
it showed some activity. I want to know whether you also have these experience,
I saw some paper they reported the released protein show 18-20 higher activity
than the fusion protein. Is it usual?
Thanks!

Huang



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