Purifying DNA from gel slices

Stefan Wirtz wirts000 at mzdmza.zdv.uni-mainz.de
Fri Nov 8 11:08:40 EST 1996


In article <Pine.OSF.3.95.961108125523.9734B-100000 at sable.ox.ac.uk>,
Alison Reavley <uzdb0017 at sable.ox.ac.uk> wrote:

> Hi, I'm looking for a method of purifying plasmid DNA from gel slices
> which does not involve phenol or any increase in temperature.  At the
> moment I am trying electroelution but find that I am precipitating agarose


The best thing we've ever tried is the Geneclean-kit by Biomol. The
procedure needs heating the sample to 45 to 55°C.
C.B.



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