BL21(DE3) pLYSe/s competent cells
Paul A Bucciaglia
bucci001 at maroon.tc.umn.edu
Fri Nov 15 12:11:59 EST 1996
I remember a decrease in competancy. As the post below states, usually
your just moving gobs of plasmid DNA to a different host. However I have
found that decent comp cells (>10EE6/ug pUC), stored frozen, will work.
ashaw5 at aol.com writes:
>Who cares? We freeze our BL21/lysS homemade competents and yes the
>efficiency is poor. (Now I think I know the reason). But you are
>generally transferring a plasmid into BL21 from another bug and so you
>don't need very efficient bugs. 100ng should easily give you enough
>colonies even if your efficiency is 10-3.
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